The changes of non-galloylated catechins and relative enzymes as well as the composition of catechins in different parts of tea shoots and in different seasons were investigated in this paper. The results showed that the contents of non-galloylated catechins except GC increased gradually from bud to the fourth leaf. Both of two synthetical enzymes related to non-galloylated cahechins, the activity of DFR/LAR declined gradually, but the activity of ANR was the highest in the third leaf. The result of correlation analysis showed that the contents of total catechins in different tea leaves presented a prominent positive correlation with the activity of DFR/LAR,but not with the activity of ANR.
ZHANG Xian-lin
,
GAO Li-ping
,
XIA Tao
,
LIU Ya-jun
,
GAO Ke-jun
. Study on the Changes of Non-galloylated Catechins and Relative Enzymes in Tea Shoots[J]. Journal of Tea Science, 2009
, 29(5)
: 365
-371
.
DOI: 10.13305/j.cnki.jts.2009.5.006
[1] 王泽农. 茶叶生物化学[M]. 北京: 农业出版社, 1980.
[2] Shirley BW.Flavonoid biosynthesis:A colorful model for genetics, biochemistry, cell biology, and biotechnology[J]. Plant Physiol, 2001, 126: 485~493.
[3] Stafford H A, Lester H H.The conversion of (L)- dihydromyricetin to its flavan-3,4-diol (leucodelphinidin) and to (L)-gallocatechin by reductase extracted from tissue cultures of Ginkgo biloba and Pseudotsuga menziesii[J]. Plant Physiol, 1985, 78: 791~794.
[4] Punyasiri PAN, Abeysinghe ISB, Kumar V.Flavonoid biosynthesis in the tea plant Camellia sinensis: properties of enzymes of the prominent epicatechin and catechin pathways[J]. Arch Biochem Biophy, 2004, 431: 22~30.
[5] Xie DY, Sharma SB, Paiva NL, et al. Role of anthocyanidin reductase, encoded by BANYULS in plant flavonoid biosynthesis[J]. Science, 2003, 299: 396~399.
[6] Xie DY, Jackson LA, Cooper JD, et al. Paiva NLMolecular and biochemical analysis of two cDNA clones encoding dihydroflavonol-4-reductase from Medicago truncatula. Plant physiol,2004, 134: 979~994
[7] Xie DY, Sharma SB, Dixon RA.Anthocyanidin reductases from Medicago truncatula and Arabidopsis thaliana[J]. Arch Biochem. Biophys, 2004, 422: 91~102.
[8] Hayashi M, Takahashi H, Tamura K, et al. Enhanced dihydroflavonol-4-reductase activity and NAD homeostasis leading to cell death tolerance in transgenic rice[J]. PNAS, 2005, 102: 7020~7025.
[9] Mamati G.E, Liang Y, Lu J. Expression of basic genes involved in tea polyphenol synthesis in relation to accumulation of catechins and total tea polyphenols[J]. J Sci Food Agric, 2006, 86: 459~464.
[10] 马春雷. 茶树查尔酮异构酶、黄酮醇合成酶和无色花色素还原酶等基因的克隆与表达分析[D]. 杭州: 中国农业科学院茶叶研究所, 2007.
[11] Li H H, Flachowsky H, Fischer TC, et al. Maize Lc transcription factor enhances biosynthesis of anthocyanins, distinct proanthocyanidins and phenylpropanoids in apple (Malus domestica Borkh.)[J]. Planta, 2007, 226: 1243~1254.
[12] Bogs J, Downey M O, Harvey J S, et al. Proanthocyanidin synthesis and expression of genes encoding leucoanthocyanidin reductase and anthocyanidin reductase in developing grape berries and grapevine leaves[J]. Plant Physiology, 2005, 139: 652~663.
浙公网安备 33019902000101号