To study the effect of theaflavin on rat mesangial cells (GMCs) were separately incubated with normal glucose, high glucose, advanced glycosylation end products (AGE) and H2O2. The protein expression of p-p38MAPK and TGF-β was examined by Western blot, and the levels of fibronectin (FN), collagen Ⅳ(ColⅣ), laminine (LN) in the supernatant of cultured GMCs were detected by ELISA. Compared to the control group, high glucose, AGE and H2O2 significantly activated p-p38MAPK and increased the protein expression of TGF-β and extracellar matrix production. The protein expression of p-p38MAPK and TGF-β was inhibited by theaflavin. Compared to GMCs incubated with corresponding stimulators, FN, ColⅣ and LN were expressed at a lower level after pre-treatment of theaflavin. It can be concluded that theaflavin may effectively attenuate diabetic nephropathy progression through down-regulating the expression of TGF-β and extracellar matrix production via p38MAPK.
LI Cai-rong
,
CAI Fei
,
ZHAO Xin-yuan
,
YANG Yin-qiao
. Effect of Theaflavin on the Activity of p38 Mitogen-activated Protein Kinase (p38MAPK) and Extracellar Matrix Production in Rat Mesangial Cells[J]. Journal of Tea Science, 2009
, 29(6)
: 470
-474
.
DOI: 10.13305/j.cnki.jts.2009.6.11
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