：Totally 281 SSRs distributed in 246 ESTs were mined out, accounting for 17.68% of 1589 ESTs updated in tea. The average length of tea plant EST-SSRs searched out is 33.06βbp and the average distance of distribution is 1/2.16βkb. The dinucleotide repeat is the dominant type with repeat motif AG/CT being the most common. 19 primer pairs for EST-SSRs were designed. After testing on the annealing temperature and the concentration of primers, dNTP and MgCl2, a suitable PCR system was established. Under the condition of reaction system developed, the primers designed were screened against genomic DNA of Longjing 43 from which most EST-SSRs were derived, and 16 primer pairs showed the amplification,accounting for 82.4% of total primers. Then the primers showing amplification were subjected to PCR for DNAs from 10 tea plant cultivars and 10 primer sets showed polymorphisms, accounting for 62.5% of primers available. Results prove that it is an effective and feasible approach to develop SSR markers based on ESTs in tea.
JIN Ji-qiang
,
CUI Hai-rui
,
CHEN Wen-yue
,
LU Mei-zhen
,
YAO Yan-ling
,
XIN Ya
,
GONG Xiao-chun
. Data Mining for SSRs in ESTs and Development of EST-SSR Marker in Tea Plant (Camellia sinensis)[J]. Journal of Tea Science, 2006
, 26(1)
: 17
-23
.
DOI: 10.13305/j.cnki.jts.2006.01.003
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