The determination of Vitamin B6 (VB6) derivated in tea plant was described by using high performance liquid chromatography with fluorescence detector. The results indicated that, the contents of VB6 in tea leaves, tender stem and roots are 3.36 μg/g, 1.27 μg/g and 0.18 μg/g fresh weight, respectively. The constituent ratio of VB6 derivatives are as follows: pyridoxine 21%, pyridoxal 2.4%, pyridoxamine 19.3%, pyridoxal 5´-phosphate 40.8% and pyridoxamine 5´-phosphate 17.9%. The content of VB6 in tea leaves increased with maturity of leaf, and decreased with aging of leaf.
JIANG Shou-hua
,
ZHANG Jian-yun
,
HUANG Long-quan
. Analysis of Vitamin B6 Derivatives in Tea Plant with High Performance Liquid Chromatography[J]. Journal of Tea Science, 2010
, 30(2)
: 79
-82
.
DOI: 10.13305/j.cnki.jts.2010.02.001
[1] Shi H, Xiong L, Stevenson B, et al. The arabidopsis salt overly sensitive 4 mutants uncover a critical role for vitamin B6 in plant salt tolerance[J]. Plant Cell, 2002(14): 575-588.
[2] Chen H, Xiong L.Pyridoxine is required for postembryonic root development and tolerance to osmotic and oxidative stresses[J]. Plant J, 2005, 44(3): 396-408.
[3] 张剑韵, 黄龙全, 早川享志, 等. 采用高效液相色谱技术分析生物体内维生素B6化合物[J]. 高等学校化学学报, 2004, 25(4): 638-640.
[4] 八木年晴. 植物中のビタミンB6濃度の上昇は植物の成長とストレス耐性に対し悪影響をもたらすのか[J]. ビタミン, 2008, 82(10): 553-555.
[5] 谭和平, 叶善蓉, 邹燕, 等. 茶叶中维生素分析方法概述[J]. 中国测试技术, 2008, 34(3): 4-8.
[6] Tsuyoshi T, Yoshio T, Takashi G.Evolution of vitamin B6 (pyridoxine) metabolism by gain and loss of genes[J]. Mol Biol Evol, 2004, 22(2): 243-250.
[7] Studart M T, Raschle T, Forster G, et al. Vitmin B6 biosynthesis in higher plants[J]. Proc Natl Acad Sci USA, 2005. 102(38): 13687-13692.
[8] Lum H K, Kwok F, Lo S.Cloning and characterization of Arabidopsis thaliana pyridoxal kinase[J]. Planta 2002, 215(5): 870-879.
[9] Sang Y, Barbosa J M, Wu H, et al. Identification of a pyridoxine (pyridoxamine) 5'-phosphate oxidase from arabidopsis thaliana[J]. FEBS Letters, 2007, 581(3): 344-348.
[10] 孙远明, 余群力. 食品营养学[M]. 北京: 中国农业大学出版社, 2002.
[11] 贾林艳, 宋伟新, 庄志萍, 等. 高效液相色谱法测定茶叶中水溶性维生素的含量[J]. 中国林副特产, 2003(2): 36-37.
[12] 熊凤麒, 袁吕江, 吴光权, 等. 高效液相色谱法同时测定茶叶中维生素C、没食子酸和咖啡碱的含量[J]. 色谱, 1993, 11(6): 366-367.
[13] 杨志洁, 叶建农, 刘彤, 等. 茶叶中维生素C和嘌呤碱的高效液相色谱测定法的研究[J]. 色谱, 1996, 14(6): 492-493.