The genetic diversity between 31 green tea cultivars and 37 Oolong tea cultivars were compared by using 26 EST-SSR markers. Results showed that number of alleles among green tea cultivars was same as that of Oolong tea cultivars except three loci amplified by primers 1036, ccmp6 and 788. Higher level of Nei’s gene diversity (H), polymorphic information contents (PIC) and average genetic distance (GD) were revealed among Oolong tea cultivars comparing to those of green tea cultivars. Based on mathematic simulation model, 68 tea cultivars were grouped into two populations, A and B. In population A, Oolong tea cultivars accounted for 67.9% of clustering samples, while in population B there had 71% of tested green tea cultivars were clustered. Whereas all tested cultivars were clustered into three populations according to Nei’s genetic distance. Oolong tea cultivars occupied 69.6% and 66.7% of clustering samples in populationⅠand Ⅱ, respectively, while 61.3% of tested green tea cultivars were clustered into population Ⅲ. These results showed that most of cultivars were clustered into the same population according to theirs processing suitability. However it is exceptive for a few of cultivars, which may be attributed to theirs improper categorization for processing suitability, geographic origin and genetic background.
WU Xiao-mei
,
YAO Ming-zhe
,
MA Chun-lei
,
WANG Xin-chao
,
CHEN Liang
. Genetic Diversity and Population Structure among Green Tea and Oolong Tea Cultivars Based on EST-SSR Markers[J]. Journal of Tea Science, 2010
, 30(3)
: 195
-202
.
DOI: 10.13305/j.cnki.jts.2010.03.008
[1] 叶乃兴. 乌龙茶种质资源的利用与品种创新. 福建茶叶[J], 2006(3): 2-4.
[2] 郭吉春, 叶乃兴, 何孝延, 等. 乌龙茶品种资源研究进展[C]. 福州: 海峡两岸茶叶科技学术研讨会论文集, 2000: 35-38.
[3] Ni S, Yao MZ, Chen L, et al. Germplasm and breeding research of tea plant based on DNA marker approaches. Front. Agric. China[J], 2008, 2(2): 200-207.
[4] 陈亮, 陈大明, 高其康, 等. 茶树基因组DNA提纯与鉴定[J]. 茶叶科学, 1997, 17(2): 177-181.
[5] 刘振, 王新超, 赵丽萍, 等. 基于EST-SSR的西南地区茶树资源遗传多样性和亲缘关系分析[J]. 分子植物育种, 2008, 6(1): 100-110.
[6] Freeman S, West J, James C, et al. Isolation and characterization of highly polymorphic microsatellites in tea(Camellia sinensis)[J]. Molecular Ecology Notes, 2004(4): 324-326.
[7] Kaundun SS, Matsumoto S.Heterologous nuclear and chloroplast microsatellite amplification and variation in tea, Camellia sinensis[J]. Genome, 2002(45): 1041-1048.
[8] Liu K, Muse SV.Integrated analysis environment for genetic marker data[J]. Bioinformatics, 2005(21): 2128-2129.
[9] Pritchard JK, Stephens M, Donnelly P.Inference of population structure using multilocus genotype data[J]. Genetics, 2000(155): 945-959.
[10] 金基强, 崔海瑞, 陈文岳, 等. 茶树EST-SSR的信息分析与标记建立[J]. 茶叶科学, 2006, 26(1): 17-23.
[11] Zhao LP, Liu Z, Chen L, et al. Generation and characterization of 24 novel EST derived microsatellites from tea plant(Camellia sinensis) and cross-species amplification in its closely related species and varieties[J]. Conservation Genetics, 2008(9): 1327-1331.
[12] Hung CY, Wang KH, Huang CC, et al. Isolation and characterization of 11 microsatellite loci from Camellia sinensis in Taiwan using PCR-based isolation of microsatellite arrays (PIMA)[J]. Conservation Genetics, 2008(9): 779-781.
[13] Yang JB, Yang J, Li HT, et al. Isolation and characterization of 15 microsatellite markers from wild tea plant(Camellia taliensis) using FIASCO method[J]. Conservation Genetics, 2009(10): 1621-1623.
[14] 黄福平, 梁月荣, 陆建良, 等. 乌龙茶种质资源种群遗传多样性AFLP评价[J]. 茶叶科学, 2004, 24(3):183-189.
[15] 刘振, 姚明哲, 王新超, 等. 基于EST-SSR的福建地区茶树资源遗传多样性和亲缘关系分析[J].中国农业科学, 2009, 42(5): 1720-1727.
[16] 罗军武, 施兆鹏, 沈程文, 等. 茶树品种资源遗传亲缘关系的RAPD分析[J]. 茶叶科学, 2002, 22(2): 140-146.
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