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Journal of Tea Science ›› 2010, Vol. 30 ›› Issue (3): 195-202.doi: 10.13305/j.cnki.jts.2010.03.008

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Genetic Diversity and Population Structure among Green Tea and Oolong Tea Cultivars Based on EST-SSR Markers

WU Xiao-mei1, YAO Ming-zhe2, MA Chun-lei2, WANG Xin-chao2, CHEN Liang2,*   

  1. 1. School of Chemistry and Life Sciences, Lishui University, Lishui 323000, China;
    2. National Center for Tea Improvement, Tea Research Institute of the Chinese Academy of Agricultural Sciences, Hangzhou 310008, China
  • Received:2009-12-17 Revised:2010-03-11 Published:2019-09-11

Abstract: The genetic diversity between 31 green tea cultivars and 37 Oolong tea cultivars were compared by using 26 EST-SSR markers. Results showed that number of alleles among green tea cultivars was same as that of Oolong tea cultivars except three loci amplified by primers 1036, ccmp6 and 788. Higher level of Nei’s gene diversity (H), polymorphic information contents (PIC) and average genetic distance (GD) were revealed among Oolong tea cultivars comparing to those of green tea cultivars. Based on mathematic simulation model, 68 tea cultivars were grouped into two populations, A and B. In population A, Oolong tea cultivars accounted for 67.9% of clustering samples, while in population B there had 71% of tested green tea cultivars were clustered. Whereas all tested cultivars were clustered into three populations according to Nei’s genetic distance. Oolong tea cultivars occupied 69.6% and 66.7% of clustering samples in populationⅠand Ⅱ, respectively, while 61.3% of tested green tea cultivars were clustered into population Ⅲ. These results showed that most of cultivars were clustered into the same population according to theirs processing suitability. However it is exceptive for a few of cultivars, which may be attributed to theirs improper categorization for processing suitability, geographic origin and genetic background.

Key words: tea, genetic diversity, population structure, EST-SSR, cultivars

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