基于代谢组学的不同年份青砖茶主要品质成分分析

doi:10.13305/j.cnki.jts.2025.01.011

Abstract

Abstract: In order to investigate the effects of storage years on the quality of Qingzhuan tea, 5 samples produced in 2022, 2019, 2014, 2009 and 2002 were analyzed by tea sensory evaluation combined with ultra-high-performance liquid chromatography quadrupole time-of-flight mass spectrometry. The sensory evaluation results show that with the extension of storage time, the appearance of Qingzhuan tea was gradually browning, the color of tea infusion was deepened, the aging flavor was progressively unveiled and the taste became more mellow, resulting in the quality improvement of Qingzhuan tea. Further metabolome analysis reveals that a total of 189 kinds of characteristic compounds were identified from Qingzhuan tea samples, which mainly included flavonoids, organic acids, amino acids, alkaloids and catechins. Moreover, 26 key differentially accumulated metabolites were identified using partial least squares-discriminant analysis (PLS-DA), including 5 alkaloids, 2 amino acids, 8 catechins, 9 flavonoids, 1 organic acid and 1 pigment substance. Among them, the contents of 8 catechin monomers decreased with the extension of storage years, while the contents of 7 flavonoids initially increased and subsequently decreased during the aging years. Hence, it was speculated that the two kinds of substances might result in the quality difference of Qingzhuan tea with different storage years. In addition, the contents of caffeic acid, lactobionic acid, linolenic acid, theophylline and betaine increased with the extension of aging years, which might also contribute to the nutrition and drinking value of Qingzhuan tea. In summary, this study analyzed the effect of aging time on the quality components of Qingzhuan tea, which shed light on the aging process of Qingzhuan tea.

Key words: Qingzhuan tea, sensory evaluation, liquid chromatography-mass spectrometry, chemical compositions, flavor

CLC Number:

MA Mengjun, HU Xinlong, QIU Shouzhe, ZHANG Ruiming, TANG Huishan, LIU Chen, YU Ziming, LI Jing, WANG Mingle. Comparative Metabolome Analysis of the Main Chemical Compositions in Qingzhuan Tea with Different Storage Years[J]. Journal of Tea Science, 2025, 45(1): 133-144.

References

[1] Lü H L, Feng X Y, Song H Z, et al.Tea storage: a not thoroughly recognized and precisely designed process[J]. Trends in Food Science & Technology, 2023, 140: 104172. doi: 10.1016/j.tifs.2023.104172.
[2] 陈玉琼, 张伟, 程倩, 等. 湖北青砖茶减肥作用研究[J]. 茶叶科学, 2008, 28(5): 363-369.
Chen Y Q, Zhang W, Cheng Q, et al.The anti-obesity effects of Hubei Qingzhuan tea on rats[J]. Journal of Tea Science, 2008, 28(5): 363-369.
[3] 陈玉琼, 张伟, 倪德江, 等. 湖北青砖茶辅助降血脂作用及其抗氧化效果[J]. 茶叶科学, 2010, 30(2): 124-128.
Chen Y Q, Zhang W, Ni D J, et al.Study on the hypolipidemic effect and antioxidative of Hubei Qingzhuan tea[J]. Journal of Tea Science, 2010, 30(2): 124-128.
[4] Cheng Q, Cai S B, Ni D J, et al.In vitro antioxidant and pancreatic α-amylase inhibitory activity of isolated fractions from water extract of Qingzhuan tea[J]. Journal of Food Science and Technology, 2015, 52(2): 928-935.
[5] Yang X H, Huang M J, Qin C Q, et al.Structural characterization and evaluation of the antioxidant activities of polysaccharides extracted from Qingzhuan brick tea[J]. International Journal of Biological Macromolecules, 2017, 101: 768-775.
[6] Liu P P, Feng L, Chen J, et al.Unlocking the secrets of Qingzhuan tea: a comprehensive overview of processing, flavor characteristics, and health benefits[J]. Trends in Food Science & Technology, 2024, 147: 104450. doi: 10.1016/j.tifs.2024.104450.
[7] Shen S S, Fu J L, Fan R Q, et al.Changes in the key odorants of loose-leaf dark tea fermented by Eurotium cristatum during aging for one year: focus on the stale aroma[J]. Food Research International, 2024, 197: 115244. doi: 10.1016/j.foodres.2024.115244.
[8] 李适, 谌滢, 傅冬和, 等. 不同年份茯砖茶感官品质研究[J]. 茶叶科学, 2016, 36(5): 500-504.
Li S, Shen Y, Fu D H, et al.Organoleptic quality analysis of Fuzhuan brick teas in different storage years[J]. Journal of Tea Science, 2016, 36(5): 500-504.
[9] Liu P P, Zheng P C, Feng L, et al.Dynamic changes in the aroma profile of Qingzhuan tea during its manufacture[J]. Food Chemistry, 2022, 375: 131847. doi: 10.1016/j.foodchem.2021.131847.
[10] 吕海鹏, 钟秋生, 王力, 等. 普洱茶加工过程中香气成分的变化规律研究[J]. 茶叶科学, 2009, 29(2): 95-101.
Lü H P, Zhong Q S, Wang L, et al.Study on the change of aroma constituents during Pu-erh tea process[J]. Journal of Tea Science, 2009, 29(2): 95-101.
[11] 侯智炜, 许姗姗, 曹琼, 等. 青砖茶主要化学成分在储存及渥堆过程中代谢分析[J]. 安徽农业大学学报, 2018, 45(4): 594-599.
Hou Z W, Xu S S, Cao Q, et al.Chemical changes of Qingzhuan tea with different storage and different pile time[J]. Journal of Anhui Agricultural University, 2018, 45(4): 594-599.
[12] Cheng L Z, Wang Y F, Zhang J R, et al.Dynamic changes of metabolic profile and taste quality during the long-term aging of Qingzhuan tea: the impact of storage age[J]. Food Chemistry, 2021, 359: 129953. doi: 10.1016/j.foodchem. 2021.
129953.
[13] 常睿, 马梦君, 罗理勇, 等. 不同年份湖北青砖茶的特征性成分分析[J]. 食品与发酵工业, 2019, 45(3): 246-253.
Chang R, Ma M J, Luo L Y, et al.Analysis of the characteristic components of Hubei Qingzhuan tea in different years[J]. Food and Fermentation Industries, 2019, 45(3): 246-253.
[14] 谌滢. 黑茶陈化过程中品质转化研究[D]. 长沙: 湖南农业大学, 2018: 11-22.
Shen Y.The research on quality variation of aging dark tea [D]. Changsha: Hunan Agricultural University, 2018: 11-22.
[15] 滕翠琴, 李锦峰, 庞月兰, 等. 不同陈化年份传统工艺六堡茶品质差异研究[J]. 安徽农业科学, 2024, 52(7): 174-177, 199.
Teng C Q, Li J F, Pang Y L, et al.Study on the quality difference of traditional process Liupao tea in different aging years[J]. Journal of Anhui Agricultural Science, 2024, 52(7): 174-177, 199.
[16] 杨春, 陈正武, 乔大河, 等. 115份贵州茶树种质茶多酚及儿茶素多样性分析及特异种质筛选[J]. 西北农业学报, 2022, 31(11): 1470-1480.
Yang C, Chen Z W, Qiao D H, et al.Diversity analysis of tea polyphenols and catechins of 115 tea plant germplasms in Guizhou and its screening of special resources[J]. Acta Agriculturae Boreali-occidentalis Sinica, 2022, 31(11): 1470-1480.
[17] 许伟, 彭影琦, 张拓, 等. 绿茶加工中主要滋味物质变化及其对绿茶品质的影响[J]. 食品科学, 2019, 40(11): 36-41.
Xu W, Peng Y Q, Zhang T, et al.Dynamic change of major taste substances during green tea processing and its impact on green tea quality[J]. Food Science, 2019, 40(11): 36-41.
[18] 罗金龙, 陈盛相, 郑文佳, 等. ‘巴山早’紫色芽叶绿茶加工过程中内含成分的变化及品质分析[J]. 四川农业大学学报, 2024, 42(2): 346-355.
Luo J L, Chen S X, Zheng W J, et al.Changes in composition and quality analysis of ‘Bashanzao’ purple bud leaf green tea during processing[J]. Journal of Sichuan Agricultural University, 2024, 42(2): 346-355.
[19] Li Q, Jin Y L, Jiang R G, et al.Dynamic changes in the metabolite profile and taste characteristics of Fu brick tea during the manufacturing process[J]. Food Chemistry, 2021, 344: 128576. doi: 10.1016/j.foodchem.2020.128576.
[20] Shen S S, Huang J L, Li T H, et al.Untargeted and targeted metabolomics reveals potential marker compounds of an tea during storage[J]. LWT, 2022, 154: 112791. doi: 10.1016/j.lwt.2021.112791.
[21] 刘盼盼, 钟小玉, 许勇泉, 等. 茶叶中有机酸及其浸出特性研究[J]. 茶叶科学, 2013, 33(5): 405-410.
Liu P P, Zhong X Y, Xu Y Q, et al.Study on organic acids contents in tea leaves and its extracting characteristics[J]. Journal of Tea Science, 2013, 33(5): 405-410.
[22] Lu J X, Wang H, Zhang Y Q, et al.Caffeic acid enhances the postharvest quality by maintaining the nutritional features and improving the aroma volatiles for nectarine fruit[J]. Food Chemistry, 2025, 464: 141633. doi: 10.1016/j. foodchem.
2024.141633.
[23] Zhang C L, Liu X, Li H Y, et al.Antibacterial mechanism of lactobionic acid against Aeromonas salmonicida by transcriptomic analysis and its application in refrigerated grass carp[J]. Food Control, 2024, 158: 110255. doi: 10.1016/j.foodcont.2023.110255.
[24] Zhu P Y, Fan L P, Yan X W, et al.Advances of α-linolenic acid: Sources, extraction, biological activity and its carrier[J]. Trends in Food Science & Technology, 2024, 152: 104676. doi: 10.1016/j.tifs.2024.104676.
[25] 罗燕, 唐玉雪, 文敏, 等.青砖茶渥堆过程中理化特性及细菌多样性分析[J]. 食品安全质量检测学报, 2022, 13(16): 5128-5136.
Luo Y, Tang Y X, Wen M, et al.Analysis of physicochemical property and bacterial diversity during the pile-fermentation of Qingzhuan tea[J]. Journal of Food Safety and Quality, 2022, 13(16): 5128-5136.
[26] 陈义, 杨转, 尹鹏, 等. 茶树新梢不同部位主要滋味物质分布规律研究[J]. 食品研究与开发, 2020, 41(13): 56-59.
Chen Y, Yang Z, Yin P, et al.Distribution of the main taste substances in different parts of tea shoots[J]. Food Research and Development, 2020, 41(13): 56-59.
[27] 马彬. 天然茶叶碱对运动员身体机能影响的研究[J]. 食品安全质量检测学报, 2020, 11(16): 5760-5765.
Ma B.Study on the effect of natural tea alkaloid on the body function of athletes[J]. Journal of Food Safety and Quality, 2020, 11(16): 5760-5765.
[28] 王国霞, 卢超, 赵奇, 等. 外源甜菜碱对低温胁迫下油茶生理特性的影响[J]. 西北林学院学报, 2020, 35(5): 78-84.
Wang G X, Lu C, Zhao Q, et al.Effects of exogenous glycine betaine on physiological characteristics of Camellia oleifera under low temperature stress[J]. Journal of Northwest Forestry University, 2020, 35(5): 78-84.

Comparative Metabolome Analysis of the Main Chemical Compositions in Qingzhuan Tea with Different Storage Years

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