A method for the determination of residual ethyl acetate in green tea extract has been studied by headspace gas chromatography in order to set up an analysis method. A wide bore HP-fast GC residual solvent column was performed, and the effects of the equilibrium temperature and equilibrium time on the determination of ethyl acetate were studied. The results showed that the standard curve was linear in the range of 0.0765~0.612μg/ml (r=0.9993). the relative standard deviation (n=6) of precision was 3.60%. the average recoveries were between 95.42% and 97.88% with RSD in the range of 1.95%~3.09%, the minimum detectable concentration(S/N=3) was 0.005μg/ml. It is a rapid, sensitive, accurate and uncontaminated method and applicable for the determination of residual ethyl acetate in green tea extract.
HU Hui-lian
,
DING Nai-li
,
WU Lian-zhen
. Determination of Residual Ethyl Acetate in Green Tea Extract by Headspace Gas Chromatography[J]. Journal of Tea Science, 2007
, 27(1)
: 67
-70
.
DOI: 10.13305/j.cnki.jts.2007.01.010
[1] 张中朋. 绿茶提取物市场巨大[J]. 中药研究与信息, 2005, 7(7): 41~41, 44.
[2] 李叶云, 江昌俊, 王秀丽. 茶多酚的生物活性及药理学研究进展[J]. 安徽中医学院学报, 2002, 21(5): 57~60.
[3] 毕彩虹, 杨坚. 茶多酚的保健作用研究进展[J]. 西南园艺, 2006, 34(2): 37~39.
[4] 王利, 翟金兰, 杨婷, 等. 茶多酚的应用及提取方法[J]. 食品研究与开发, 2006, 27(3): 154~156.
[5] 黄秋森. 气相色谱法测定茶多酚中的氯仿和乙酸乙酯残留[J]. 福建分析测试, 2005, 14(2): 2173~2174, 2172.
[6] 冯信平, 谢德芳. 气相色谱法测定茶多酚中乙酸乙酯[J]. 热带农业科学, 2001(6): 8~10.
浙公网安备 33019902000101号