According to nucleotide sequences of the two key genes, A13-1 lef-8 gene and A13-1 polyhedrin gene of EpNPV, two pairs of special primers were designed. Two typical DNA fragments with the size of 538 bp and 281 bp were amplified by PCR method. The two PCR products were cloned into T vector and sequenced, Blasted with the DNA sequence of EpNPV, both of them were matched absolutely. That meant that these two DNA fragments can be used as molecule markers to identify the Euproctis pseudoconspersa Strand infected with EpNPV. The present investigation constructed a molecular biological method to detect EpNPV DNA, to evaluate the biological control effect of EpNPV and to study the infecting route of EpNPV into E. pseudoconspera.
FU Jian-yu
,
HAN Bao-yu
. Rapid Molecular Identification of Euproctis pseudoconspersa Infected with EpNPV by PCR Method[J]. Journal of Tea Science, 2008
, 28(4)
: 255
-259
.
DOI: 10.13305/j.cnki.jts.2008.04.006
[1] 谭济才. 茶树病虫防治学[M]. 北京: 中国农业出版社, 2002.
[2] 厉晓蜡, 金轶伟, 柴一秋, 等. 茶毛虫核型多角体病毒对茶毛虫的致病性研究[J]. 茶叶科学, 2006, 26(4): 265~269.
[3] 张汉鹄,卜可华. 茶毛虫的发育起点与有效积温[J]. 茶叶科学, 1987, 7(1): 41~44.
[4] 姚渭, 低位侧向喷施茶毛虫NPV技术及大田应用试验[J]. 茶叶科学, 1987, 1(2): 52~53.
[5] 殷坤山, 肖强. 茶毛虫病毒杀虫剂田间使用技术研究[J]. 中国茶叶, 2004, (3):18~19.
[6] 常国辉, 陈绳亮, 罗保君, 等. 茶毛虫核型多角体病毒(EpNPV)多角体蛋白基因的定位及克隆[J]. 中国病毒学, 2001, 16(4): 390~392.
[7] 陈绳亮, 罗保君, 常国辉, 等. 茶毛虫核型多角体病毒基因组酶切分析及质粒文库的构建[J]. 华中农业大学学报, 1999, 18(4): 303~306.
[8] 聂婷婷, 肖强, 殷坤山, 等. 茶毛虫NPV的P24、Rr1、Lef-81基因及其分子进化分析[J]. 茶叶科学, 2005, 25(4): 242~248.
[9] Dall D, Sriskantha A.A gene encoding a highly expressed spindle body protein of Heliothis armigera entomopoxvirus[J]. J Gen Virol, 1993, 74: 1811~1818.
[10] 王礼中,肖强,张传溪. 茶毛虫核型多角体病毒ph基因抗体的制备与利用[J]. 茶叶科学, 2008, 28(4): 260~266.
[11] 戈峰, 王利军, 王常平, 等. 茶毛虫性信息素对茶毛虫防治效果研究[J]. 茶叶科学, 2002, 22(2): 115~118.
浙公网安备 33019902000101号