Determination of nitrogen concentrations in tissues is an important diagnostic means for N status of plants. A pot experiment with tea plants applied with different amounts of N fertilizer was carried out to evaluate the usefulness of SPAD-502 as a diagnostic tool. The results showed that the yield of young shoots and N concentration of mature leaves responded to N application rates with a pattern of an initial linear increase before a platform was reached. There was a significantly positive linear relationship between the SPAD reading and total N concentration of mature leaves. The similar pattern of an initial linear increase followed by a platform was observed for the relationship between SPAD reading and the yield. These results suggested that the N status of tea plants may be characterized by the measurement of SPAD reading of mature leaves and the SPAD is a potentially useful rapid diagnostic tool in N fertilization recommendation. However, this needs further validation from field experiments. Those factors which may influence the precision of SPAD measurement, such as the point of test on a leaf, selection of leaves from twig (position), weather condition, in situ or in vitro analysis, surface cleaning and the number of leaves for a composite sample were also investigated.
YANG Yi-yang
,
MA Li-feng
,
SHI Yuan-zhi
,
RUAN Jian-yun
,
LI Xing-hui
. Evaluation of Nitrogen Status in Tea Plants by SPAD[J]. Journal of Tea Science, 2008
, 28(4)
: 301
-308
.
DOI: 10.13305/j.cnki.jts.2008.04.007
[1] 李志宏, 张云贵, 刘宏斌, 等. 叶绿素仪在夏玉米氮营养诊断中的应用[J]. 植物营养与肥料学报, 2005, 11(6): 764~768.
[2] 朱新开, 盛海君, 顾晶, 等. 应用SPAD值预测小麦叶片叶绿素和氮含量的初步研究[J]. 麦类作物学报, 2005, 25(2): 46~50.
[3] Minotti P L, Halseth D E, Sieczka J B.Field chlorophyll measurements to asses the nitrogen status of potato varieties[J]. Hort Sci, 1994, 29(12): 1497~150.
[4] Piekielek W P, Fox R H.Use of a chlorophyll meter to predict side-dress nitrogen requirement for maize[J]. Agron J, 1992, 84:59~65.
[5] Singh B, Singh Y, Ladha J K et al. Chlorophyll meter and leaf color cart based nitrogen management for rice and wheat in Northwestern India[J]. Agron J, 2002, 94(4): 821~829.
[6] Turner F T, Jund M F.Chlorophyll meter to predict nitrogen top-dress requirement for semidwart rice[J]. Agron J, 1991, 83: 926~928.
[7] Waskom R M, Westfall D G, Spellman D E, et al. Monitoring nitrogen status of corn with a portable chlorophyll meter[J]. Commun Soil Sci Plant Anal, 1996, 27(3~4): 545~560.
[8] Wood C W, Tracy P W, Reeves D W, et al. Determination of cotton nitrogen status with a hand-held chlorophyll meter[J]. J Plant Nutr, 1992, 15(9): 1435~1448.
[9] Chapman S C, Barreto H J.Using a chlorophyll meter to estimate specific leaf nitrogen of tropical maize during vegetative growth[J]. Agron J, 1997, 89: 557~562.
[10] Campbell R J, Mobley K N, Marini R P, et al. Growing conditions alter relationship between SPAD-501 values and apple leaf chlorophyll[J]. Hort Sci, 1990, 25: 330~331.
[11] Scott X, Changa, Daniel J, et al. Nondestructive and rapid estimation of hardwood foliar nitrogen status using the SPAD-502 chlorophyll meter[J]. Forest Ecol Manag, 2003, 181: 331~338.
[12] 李合生. 植物生理生化实验原理和技术[M]. 北京: 高等教育出版社, 2000.