Differences of gene expression between normal and albino leaves of tea plant (Camellia sinensis cv. Baiye1) were studied by mRNA differential display reverse transcription-PCR (DDRT-PCR). Fifty-eight differentially expressed cDNA fragments were obtained from normal and albino leaves. Twelve of which were confirmed by the analysis of RT-PCR. Among them, 5 fragments were specially expressed in normal leaves, 4 fragments were specially expressed in albino leaves, 1 fragment was up-regulated in normal leaves, and 2 fragments were up-regulated in albino leaves. Through BLASTX analysis with GenBank, 5 cDNA fragments showed high homologous to five known genes, namely soul heme-binding family protein of Arabidopsis thaliana, methionine synthase of Beta vulgaris, gag-pol polyprotein-related in Medicago truncatula, human glyceraldehyde-3-phosphate dehydrogenase pseudogene and ACC synthase of rose, respectively. The other 7 might be novel genes, they showed poor homologous to all proteins in the database.
WANG Xin-chao
,
ZHAO Li-ping
,
YAO Ming-zhe
,
CHEN Liang
,
YANG Ya-jun
. Preliminary Study on Gene Expression Differences between Normal Leaves and Albino Leaves of Anji Baicha (Camellia sinensis cv. Baiye1)[J]. Journal of Tea Science, 2008
, 28(1)
: 50
-55
.
DOI: 10.13305/j.cnki.jts.2008.01.010
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