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The Effect of Different Extracts of Fuzhuan Tea on the Activities of Digesting Enzyme

  • FU Dong-he ,
  • LIU Zhong-hua ,
  • HUANG Jian-an ,
  • CHEN Hui-heng
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  • 1. Tea Key Lab of the Ministry of National Teaching, Changsha 410128, China;
    2. College of Horticulture and Landscape, Hunan Agricultural University, Changsha 410128, China

Received date: 2007-07-20

  Revised date: 2007-09-17

  Online published: 2019-09-16

Abstract

Fuzhuan Tea was extracted by chloroform, ethyl acetate, 1-butanol in turn. The effect of Fuzhuan Tea on the activities of metabolize enzyme was investigated. The results showed that different fractions of Fuzhuan Tea had different effects on pancreatic lipase activity. The sample extracted by chloroform could restrain pancreatic lipase a little and the relative activity was 0.82. The water extracts, the sample extracted by ethyl acetate and 1-butanol showed obvious activation, that of the sample extracted by 1-butanol was the most active. The multiple was 2.54. All fractions of Fuzhuan Tea were active to α-amylase, among them, the sample extracted by ethyl acetate was the most active. The relative activiy was 4.09. The water extracts was the second one and its relative activity was 2.99. That of the layer of water sample was not obvious. The sample extracted by chloroform could restrain α-amylase a little and the relative activity was 0.88. The samples separated from the sample extracted by ethyl acetate and the sample extracted by 1-butanol were active to α-amylase and pancreatic lipase respectively.

Cite this article

FU Dong-he , LIU Zhong-hua , HUANG Jian-an , CHEN Hui-heng . The Effect of Different Extracts of Fuzhuan Tea on the Activities of Digesting Enzyme[J]. Journal of Tea Science, 2008 , 28(1) : 62 -66 . DOI: 10.13305/j.cnki.jts.2008.01.003

References

[1] 屠幼英, 须海荣, 梁惠玲, 等. 紧压茶对胰酶活性和肠道有益菌的作用[J]. 食品科学, 2002, 23(10): 113~116.
[2] 吴媛媛, 屠幼英, 陈文峰, 等. 紧压茶对α-淀粉酶促活作用的研究[J]. 中国茶叶加工, 2002, 16(1): 38~39.
[3] 张盛, 刘仲华, 黄建安, 等. 吸附树脂法制备高纯儿茶素的研究[J]. 茶叶科学. 2002, 22(2): 125~130.
[4] 王坤波, 刘仲华, 黄建安. 儿茶素体外氧化制备茶黄素的研究[J]. 茶学科学, 2004, 24(1): 53~59.
[5] 黄意欢. 茶学实验技术[M]. 北京: 中国农业出版社, 1995.
[6] The Amylase Research Society of J apan (Ed). Handbook of Amylase and Related Enzymes[M]. Oxford: Oxford Pergamon Press, 1988: 53~56.
[7] 周景祥, 王桂芹, 余涛. 蛋白酶和淀粉酶活性检测方法探讨[J]. 中国饲料, 2001, 11: 23~24.
[8] 曹建明, 谢作叙, 周铁丽. 改良的淀粉酶测定法[J]. 陕西医学检验, 2001, 16(3): 63.
[9] Gotz F, Poop F, Schleifer K H.Complete nucleotide sequence of the lipase gene from staphylococcus hyicus cloned in staphylococcus hyicus[J].Nucleic Acid Res., 1985, 13: 5895~5906.
[10] 屠幼英, 梁慧玲, 陈暄, 等. 紧压茶儿茶素和有机酸的组成分析[J]. 茶叶, 2002, 28(1): 22~24.
[11] Datta S, Lou C, Li W, et a1.Human hepaticlipase[J]. J Biol Chem, 1988, 263: 1107~1110.
[12] Winkler F K, D'Arcy A, Hunzuker W. Structure of human pancreatic lipase[J]. Nature, 1990, 343: 771~774.
[13] Huang A H C.In Lipases (Borgströn B and Brockman H L, eds) Elsevier[M]. Amsterdam, 1984: 365~416.
[14] Cardenas J, Alvarez E,de Castro-Alvarez M-S, et a1.Screening and catalytic activity in organlc synthesis of novel fungal and yeast lipase[J]. J Mol Catal B, 2001, 14: 111~123.
[15] Osada K, Takahashi K, Hatano M.Polyunsaturated fatty acid glyceride syntheses by microbial lipase[J]. J Am 0il Chem Soc, 1990, 67: 921~922.
[16] Kaimal T N B, Saroja M. Enhancement of cata1ytic activity of porcine pancreatic lipase by reductive alkylation[J]. Biotechno1 lett, 1989, 11: 31.
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