To investigate the protective effect of Camellia angustifolia Chang extracts (CA. extracts) on restraint stress-induced liver damage in mice, The mice model of stress-induced liver damage was used in investigation. After 18βh restraint stress on mice 4 days following CA. extracts treatment, the liver damage was established. Alanine aminotransferase (ALT) and xanthine oxidase(XOD)activities, malondialdehyde (MDA) contents, GSH levels, GPX and GST activities, as well as oxygen radical absorbance capacity (ORAC) values in liver were determined to evaluate the hepatoprotective effects. Compared with restraint model group, CA. extracts can markedly reduce ALT activities, MDA contents and XOD activities, protect the liver damage induced by oxidative stress. Additionally, CA. extracts can effectively increase the ORAC values both in vivo and in vitro, as well as GSH levels, GPX activities and GST activities. Oral treatment of CA. extracts was found to reduce restrain-stress-induced liver damage in terms of above mentioned biochemical parameters, it is considered that this protective effects may be related to its free radical scavenging activity and lipid peroxidation inhibitory effects.
GU Chen-fei
,
BAO Li
,
XIE Guo
,
LU Yan-qing
,
YAO xin-sheng
,
Kurihara Hiroshi
. Protective Effects of Camellia angustifolia Chang on Oxidative Stress-induced Liver Damage in Mice[J]. Journal of Tea Science, 2008
, 28(2)
: 115
-122
.
DOI: 10.13305/j.cnki.jts.2008.02.013
[1] 张宏达. 山茶属植物的系统研究[J]. 中山大学学报(自然科学)论丛, 1981: 119~120
[2] 续洁琨. 茶组四种植物化学成分的比较及生物碱theacrine对中枢神经系统作用的研究[D]. 沈阳药科大学博士学位论文, 2006.
[3] Kurihara H, Koda H, Asami S, et al. Contribution of the antioxidative property of astaxanthin to its protective effect on the promotion of cancer metastasis in mice treat with restraint stress[J]. Life Science, 2002, 70:2509~2520.
[4] Yamaoka Y, Kawakita T, Nomoto K.Protective effect of a traditional Japanese medicine, Bu-zhong-yi-qi-tang (Japanese name: Hochu-ekki-to), on the restraint stress-induced susceptibility against Listeria onocytogenes[J]. Immunopharmacology, 2000, 48: 35~42.
[5] Hiraku Y, Murata M, Kawanishi S.Determination of intracellular glutathione and thiols by high performance liquid chromatography with a gold electrode at the femtomole level: comparison with a spectroscopic assay[J]. Biochimica et Biophysica Acta-bioenergeics, 2002, 1570: 47~52.
[6] Ou B, Hampsch-Woodill M, Prior R L.Development and validation of an improved oxygen radical absorbance capacity assay using fluorescein as the fluorescent probe[J]. Journal of Agricultural and Food Chemistry. 2001, 49: 4619~4626.
[7] Davalos A, Gomez-Cordoves C, Bartolome B.Extending Applicability of the Oxygen Radical Absorbance capacity (ORAC-Fluorescein) Assay[J]. Journal of Agricultural and Food Chemistry, 2004, 52: 48~54.
[8] Kurihara H, Fukami H, Asami S, et al. Effects of Oolong Tea on plasma Antioxidative Capacity in Mice Loaded with Retraint Stress Assessed Using the Oxygen Radical Absorbance Capacity(ORAC)Assessed[J]. Biological & Pharmaceutical Bulletin, 2004, 27: 1093~1098.
[9] H A Zar, K Tanigawa, Y M Kim, et al. Rat liver Postischemic lipid peroxidation and vasocon- striction depend on Ischemia time[J]. Free Radical Biology & Medicine 1998, 25: 255~264.
[10] Fournier D, Bride J M, Poirie M, et al. Insect glutathione S-transferases,Biochenmical characteristics of major forms from houseflies susceptible and resistant to insecticides[J]. Joumal of Biological Chemistry, 1992, 267: 1840~1845.
[11] Peters L D, Livingatone D R.Antioxidant enzyme activities in embryolobic and early larval stages of turbot[J]. Jounal of Fish Biology, 1996, 49: 986~997.
[12] Mayne S T.Antioxidant Nutrients and Chronic Disease: Use of Biomarkers of Exposure and Oxidative Stress Status in Epidemiologic Research[J]. Journal of Nutrition, 2003, 133(3): 933~940.
[13] Kavita G, Ayanabha C, Arunabha R Modulation of stress-induced neuro behavioral changes and brain oxidative injury by nitric oxide(NO) mimetics in rats[J]. Behavioural Brain Research. 2007, 182: 226~230.
[14] Yilmaz Y, Toledo R T.Major flavonoids in grape seeds and skins: Antioxidant capacity of catechin, epicatechin, and gallic acid[J]. Journal of Agricultural and Food Chemistry, 2004, 52: 255~260.
浙公网安备 33019902000101号 
