In order to screen special germplasm resources of tea plant (Camellia sinensis (L.) O. Kuntze) with high ester-catechin content, 780 tea plant resources in the Tea Variety Garden of Fujian were utilized as material. According to the appearance of tea plant, 45 tea plant resources were screened as candidate. The ester-catechin contents of spring tea shoots, summer tea shoots and autumn tea shoots in 45 cultivars or lines were analyzed by isocratic elution HPLC. The result was as follows: ester-catechin content of tea plant shoots varied greatly with cultivars or lines. In spring tea shoots, summer tea shoots, autumn tea shoots, and the ester-catechin contents in 45 cultivars or lines were in the range of 5.21%~24.03%、3.05%~20.31%、2.89%~19.66%, averaged 16.08%、14.73%、9.96%, respectively. The variation coefficients were 22.26%、24.10%、40.76%, respectively. The ester-catechin contents in the shoot of the same cultivars in different season, mostly: spring tea shoots >summer tea shoots >autumn tea shoots. There were 5 cultivars and lines(033、505、509、524、1005), which the annual average ester-catechin contents exceeded 19%. These could be considered as special tea plant germplasm resources with high ester-catechin contents and be used as important materials of both quality genetic improvement and the extraction of ester-catechin from tea plant. There were 8 cultivars and lines(033、505、509、510（1）、510（2）、524、1001、1005), which the ratio of ester-catechin contents to total catechins content exceeded 80%（ordinary level is 68%）. These could be used as special materials for extracting the tea polyphenols or catechins.
LIN Jin-ke
,
CHEN Rong-bin
,
CHEN Chang-song
,
YOU Xiao-mei
,
ZHANG Ying-gen
,
CHEN Lin
,
LI Xiu-feng
. A Study on Screening Germplasm Resources of Tea Plant with High Ester-Catechin Content[J]. Journal of Tea Science, 2005
, 25(1)
: 30
-36
.
DOI: 10.13305/j.cnki.jts.2005.01.006
[1] 安徽农业大学. 茶叶生物化学[M]. 北京: 农业出版社, 第二版, 1994: 86~117.
[2] Jankun J, Selman S H, Swiercz R.Why drinking green tea cold prevent cancer[J]. Nature, 1997, 387(6633): 561.
[3] Xiaohua T, Darong H U.Differences of four catechins in human cell cycle arrest and induction of apoptosis in Lovo cells[J]. Cancer Letters, 2000, 158: 40.
[4] 辛华雯, 曾繁典, 谢笔钧. 聚酯型儿茶素抗癌作用的机制研究[J]. 中国药理学会通讯, 2001, 18(4): 18~18.
[5] 赵保路. 茶多酚的抗氧化作用[J]. 科学通报, 2002, 47(16): 1206-1210
[6] Huang H, Xu X Q.Anticancer Activity of Tea: Evidence from Recent Animal Experiments and Human Studies[J](英文). 茶叶科学, 2004, 24(1): 1~11.
[7] 黄建安, 施兆鹏, 施英, 等. 乌龙茶“岩韵”与“音韵”的感官体验及化学特性[J]. 湖南农业大学学报, 2003, 29(2): 134~136.
[8] 浙江农业大学主编. 茶树栽培学[M]. 北京: 中国农业出版社, 第二版, 1996, 92~102; 296.
[9] 唐课文, 周春山, 蒋新宇. 沉淀-吸附法制备高酯型儿茶素[M]. 中南工业大学学报, 2002, 33(3): 247~249.
[10] 孙智达, 谢笔钧. 高酯型儿茶素茶多酚制备方法的研究[J]. 华中农业大学学报, 1995, 14(5): 495~503.
[11] 杜琪珍, 李名君, 程启坤, 等. 茶儿茶素单体的制备及其酯型儿茶素转化成简单儿茶素的研究[J]. 茶叶科学, 1997: 125~131.
[12] 程琦, 程启坤, 李名君. 单宁酶的固定化及其在酯型儿茶素水解反应中的应用[J]. 生物化学杂志, 1996, 12(4): 423~426.
[13] 辛华雯, 史霄燕, 曾繁典, 等. 聚酯型儿茶素的减毒作用研究[J]. 中国药师, 2002, 5(11): 650~651.
[14] 杨亚军. 品种间茶多酚含量差异及其与茶叶品质关系的探讨[J]. 中国茶叶, 1989(5): 8~10.
[15] 刘祖生, 赵东. 中国大陆茶树育种的主要成就与基本经验.第二届海峡两岸茶叶科技学术研讨会论文集[A], 台湾, 2001, 240~147.
[16] Liang Y R, Tanaka J C, Takeda. Y S, Wachira F. Application of RAPD Markers to Research on Genetic Resources of Tea Plant. In:Proceeding of the International Symposium on Molecular Biology and Tea Breeding[A]. Hangzhou: Zhejian University. 2000: 60~70.
[17] 罗军武, 施兆鹏, 沈程文, 等. 茶树品种资源遗传亲缘关系的RARD分析[J]. 茶叶科学, 2002, 22(2): 140~146.
[18] 江昌俊, 李卓民, 房国宾. 利用N+离子诱变选育茶树良种初报[J]. 安徽农业大学学报(自然科学版), 2000, 27(1): 48~50.
[19] 陈亮, 王平盛, 山口聪. 应用RAPD分子标记鉴定野生茶树种质资源研究[J]. 中国农业科学, 2002, 35(10): 1186~1192.
[20] 陆锦时. 茶树儿茶素含量及组成特性与品种品质的关系[J]. 西南农业学报, 1994, 7(增刊): 6~12.
[21] 虞富莲, 俞永明, 李名君, 等. 茶树优质资源的系统鉴定与综合评价[J]. 茶叶科学, 1992, 12(2): 95~125.
[22] 俞永明. “八五”期间茶种质源研究中筛选出一批优质资源[J]. 中国茶叶, 1996(3): 26.
[23] 杨亚军, 虞富莲, 陈亮, 等. 茶树优异资源评价与遗传稳定性研究[J]. 茶叶科学, 2003, 23(1): 6~8.
[24] 黄意欢. 茶学试验技术[M]. 北京: 农业出版社, 1997: 231~232.
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