Welcome to Journal of Tea Science,Today is

Occurrence of Bacillus thuringiensis on the Phylloplane and Screening of Highly Toxic Strains to Tea Pests

  • ZHANG Ling-ling ,
  • LIN Jin ,
  • LUO Lan ,
  • FANG Fang ,
  • HUANG Tian-pei ,
  • XU Jin-han ,
  • WU Guang-yuan ,
  • WANG Qin-sen ,
  • GUAN Xiong
Expand
  • 1. Key Laboratory of Biopesticide and Chemical Biology, Ministry of Education, Fujian Agricultural and Forestry University, Fuzhou 350002, China;
    2. Tea Research Institute, Fujian Academy of Agricultural Sciences, Fu’an 355015, China

Online published: 2019-09-16

Abstract

An isolation method was developed with comparing different selective culture media and 54 Bt strains were isolated from 25 species of plants in China. The bioassay was conducted by use of Ectropis obliqua, Empoasca vitis and Acaphylla theae as target insects. It showed that there were 10 strains effective to Lepidoptera and 4 to Homoptera but they were all ineffective to Acaphylla theae mites.

Cite this article

ZHANG Ling-ling , LIN Jin , LUO Lan , FANG Fang , HUANG Tian-pei , XU Jin-han , WU Guang-yuan , WANG Qin-sen , GUAN Xiong . Occurrence of Bacillus thuringiensis on the Phylloplane and Screening of Highly Toxic Strains to Tea Pests[J]. Journal of Tea Science, 2005 , 25(1) : 56 -60 . DOI: 10.13305/j.cnki.jts.2005.01.010

References

[1] Ercolane G.L. Pseudomonas savastanoi and Other Bacteria Colonizing the Surface of Olive Leaves in the Field[J]. Journal of General Microbiology, 1978, 109: 245~257.
[2] Yoshmura F.Phylloplane bacteria in a pine forest[J]. Can. J. Microbiol, 1982, 28: 580~592.
[3] Robert A.Smith and Graham A, et al. The Phylloplane as a Source of Bacillus thuringiensis Variants[J]. Applied Environmental Microbiology, 1991: 311~315.
[4] Ohba M.Bacillus thuringiensis population naturally occurring on mulberry leaves: a possible source of the populations associated with silkworm-rearing insectaries[J]. J Appl Bacteriol, 1996, 80: 56~64.
[5] P.H.Damgaard, B.M.Hansen, J.C.Pedersen, et al. Natural occurrence of Bacillus thuringiensis on cabbage foliage and in insects associated with cabbage crops[J]. Journal of Applied Microbiology, 1997, 82: 253~258.
[6] P.H.Damgaard, A.Abdel-Hameed, J.Ellenberg, et al. Natural occurrence of Bacillus thuringiensis on grass foliage[J]. World Journal of Microbiology & Biotechnology, 1998, 14: 239~242.
[7] Rizali, Asano, Sahara, et al. Novel Bacillus thuringiensis serovar aizawai strains isolated from mulberry leaves in Indonesia[J]. Appl Entomol Zool, 1998, 33(1): 111~114.
[8] Mizuki, Ichimatsu, Hwang, et al. Ubiquity of Bacillus thuringiensis on phylloplanes of arboreous and herbaceous plants in Japan[J]. Journal of Applied Microbiology, 1999, 86: 979~984.
[9] Pascale Kaelin and Ferruccio Gadani. Occurrence of Bacillus thuringiensis on Cured Tobacco Leaves[J]. Current Microbiology,2000, 40: 205~209.
[10] P.Maduell, R. Callejas, K. R. Cabrera, et al. Distribution and Characterization of Bacillus thuringiensis on the Phylloplane of Species of Piper (Peperaceae) in Three Altitudinal Levels[J]. Microbiol. Ecol., 2002, 44: 144~153
[11] 关雄. 苏云金芽孢杆菌8010的研究[M]. 科学出版社, 1997: 1~6.
[12] 喻子牛. 苏云金芽孢杆菌制剂的生产和应用[M]. 农业出版社, 1990: 32~34.
[13] 杨自文, 吴宏文, 王开梅, 等. 从土壤中高效分离苏云金杆菌的方法[J]. 中国生物防治, 2000, 16(1): 26~30.
[14] 李建洪, 万秋英, 王沫, 等. 韩国土壤中苏云金芽孢杆菌菌株的分离和鉴定[J]. 湖南农业大学学报, 2000, 25(4): 293~295.
[15] Russell S.T, P.A.W.Martin, C.F.Reichelderfer. Selective process for efficient isolation of soil Bacillus spp[J]. Appl Environ Microbiol, 1987, 53(6): 1263~1266.
[16] 陈在佴. 一种从土壤中分离苏云金杆菌的新方法[J]. 中国生物防治,1996,12(2): 75~77.
[17] 何献君, 宗浩, 郑鸽, 等. 一株苏云金芽孢杆菌(Bacillus thuringiensis)的分离与鉴定[J].四川师范大学学报(自然科学版), 2002, 25(3): 301~303.
[18] 李凤梅, 李平, 闫敏, 等. 苏云金芽孢杆菌的筛选和初步鉴定. 生物技术, 2003, 13(6): 34~36.
[19] 张灵玲,姚凤銮,黄天恩, 等. 营养型生长调节剂TA-BR制备液的研制及其在茶叶上的应用[J]. 福建农林大学学报(自然科学版), 2004, 33(6): 310~312.
[20] 张灵玲, 姚凤銮, 黄志鹏, 等. TA-BR生物药肥的研制及在茶叶上的应用[J]. 茶叶科学,2004, 24(3): 219~225.
Outlines

/