Tea anthracnose fungi, Gloeosporium theae-sinensis, can produce pathogenic exo-toxin, which induced the lesions of tea leaves and the symptoms being similar to those resulted from G. theae-sinensis in the fields. The cultural filtrate of G. theae-sinensis was used to toxin bioassay, so as to make a study of the optimum conditions for toxin-producing and characteristics of toxins. The optimum toxin-producing conditions were as follows: pH 5.0, and 25℃ cultivating for 16 days in the manner of vibration with Czapek-Dox liquid medium. The method of immerging the tea shoots into toxin solution was appropriate for the bioassay of toxins.
LIU Shou-an
,
HAN Bao-yu
,
FU Jian-yu
,
CUI Lin
,
LI Gang
. Preliminary Studies on Pathogenic Activity and Physical and Chemical Characteristics of Toxins from Gloeosporium theae-sinensis[J]. Journal of Tea Science, 2007
, 27(2)
: 153
-158
.
DOI: 10.13305/j.cnki.jts.2007.02.010
[1] 张勇, 宋明顺. 我国茶叶质量安全标准问题的探讨[J]. 浙江农业科学, 2003(6): 14~17.
[2] 蔡军. 2005年我国茶叶出口情况浅析及展望[J]. 中国茶
叶, 2006(2): 7~9.
[3] Anderson A J.Isolation from three species of Colletotrichum of glucan-containing polysaccharides that elicit browning and phytoalexin production in bean[J]. Phytopathology, 1978, 68(2): 189~194.
[4] Frantzen M M, Johnson L B, Stuteville D L.Partial characterization of phytotoxic polysaccharides produced in vitro by Colletotrichum trifolli[J]. Phytopathology, 1982, (72): 568~573.
[5] 宋佐衡. 植物病理生理学[M]. 北京: 农业出版社, 1991.
[6] 曲玲, 曹有龙. 枸杞炭疽病菌毒素的初步研究[J]. 植物保护, 2004, 30(5): 65~68.
[7] 王敬文. 普通油茶炭疽病菌体外产生的毒素[J]. 植物保护学报, 1986, 13(3): 151~157.
[8] 欧阳丰, 谢丙炎, 欧阳本友, 等. 辣椒炭疽病菌毒素[J]. 真菌学报, 1993, 1(4): 289~296.
[9] 张广民, 王智发, 刘延荣, 等. 烟草低头黑病菌培养滤液对烟草毒性及作用特性的研究[J]. 山东农业大学学报, 1995, 26(2): 130~136.
[10] 董金皋. 真菌毒素生物测定方法研究概况Ⅰ植株和组织器官水平的生物测定[J]. 河北农业大学学报, 1992, 15(4): 99~102.
[11] 陈宗懋, 陈雪芬. 茶树病害的诊断和防治[M]. 上海: 上海科学技术出版社, 1989.
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