In order to detect various pesticide residues in tea, a reliable, rapid and high throughput detection method was established: a solid phase extraction was developed for the determination of 79 pesticide residues in tea by gas chromatography-tandem mass spectrometry (GC-MS/MS) and liquid chromatography-tandem mass spectrometry (LC-MS/MS). These samples were extracted by 20βmL acetonitrile. 10βmL extracts were cleaned by Carbon/NH2 column with 20βmL acetonitrile + toluene (3∶1) and then mixed with 5βmL acetonitrile, then these procdssed samples were detected by GC-MS/MS and LC-MS/MS, respectively. The results show that the concentrations of 79 pesticide residues had good linearity relationships in the range of 0.01-0.40βmg·L-1, with the correlation coefficients (R2) above 0.995, the recoveries of high, low and medium levels were ranged from 67.3%-130.8%, the relative standard deviations (RSDs) were less than 15%, the limits of quantification (LOQs) of 98.7 % pesticides were less than or equal to 0.01βmg·kg-1, multiple types of tea were detected, and the results were proved to be qualified. Consequently, the method can simultaneously detect 5 kinds of pesticides, including organophosphorus, organochlorine, pyrethroids, carbamates and organic heterocyclic pesticides, which would be useful to determine multi-pesticide residues in high numbers of tea samples.
ZENG Yan
,
LANG Hong
,
YANG Qiaohui
,
LI Xiaxue
,
SHI Chaoting
. Determination of 79 Pesticide Residues in Tea by Solid Phase Extraction with GC-MS/MS and LC-MS/MS[J]. Journal of Tea Science, 2019
, 39(5)
: 576
-586
.
DOI: 10.13305/j.cnki.jts.2019.05.009
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