Screening, Identification and Culture Conditions of a High-virulent Isolate of Beauveria against Myllocerinus Aurolineatus

WANG Dingfeng; LI Liangde; LI Huiling; ZHANG Hui; WU Guangyuan

doi:10.13305/j.cnki.jts.2021.01.009

2021 , Vol. 41 >Issue 1: 101 - 112

DOI: https://doi.org/10.13305/j.cnki.jts.2021.01.009

Research Paper

Screening, Identification and Culture Conditions of a High-virulent Isolate of Beauveria against Myllocerinus Aurolineatus

WANG Dingfeng ,
LI Liangde ,
LI Huiling ,
ZHANG Hui ,
WU Guangyuan

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Tea Research Institute, Fujian Academy of Agricultural Sciences, Fuzhou 350013, China

Received date: 2020-07-21

Revised date: 2020-11-01

Online published: 2021-02-23

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Abstract

Myllocerinus aurolineatus (Coleoptera: Curculionidae) is one of the most important pests in tea plantation, and chemical control is the usual treatment method for this weevil at present. In order to explore alternative strategy for sustainable control of the weevil, dip method was employed to test the virulence of 12 isolates of Beauveria sp., which isolated from insects of Coleoptera. When inoculated with spore suspension (5.0×10⁷ spores·mL^-1), the strain Bbr1552 showed the highest virulence against the adults of M. aurolineatus, which caused the highest corrected mortality rates of 100%, shortest LT₅₀ of 4.49 d and highest cadaver rates of 69.44%. The LC₅₀ value was 1.55×10⁶ spores·mL^-1 on 7 d (25℃) after inoculation with Bbr1552. According to morphological identification and analysis of rDNA-ITS and Bloc sequences, the strain Bbr1552 was identified to be a B. brongniartii. Besides, the PPDA medium and 25℃ were the most optimal conditions for the colony growth and sporulation of the strain Bbr1552.

Key words： Myllocerinus aurolineatus; Beauveria brongniartii; virulence; identification; cultural condition

Cite this article

WANG Dingfeng , LI Liangde , LI Huiling , ZHANG Hui , WU Guangyuan . Screening, Identification and Culture Conditions of a High-virulent Isolate of Beauveria against Myllocerinus Aurolineatus[J]. Journal of Tea Science, 2021 , 41(1) : 101 -112 . DOI: 10.13305/j.cnki.jts.2021.01.009

References

[1] 曾明森, 吴光远, 王庆森. 我国茶丽纹象甲的研究进展[J]. 河南科技大学学报(农学版), 2003, 23(4): 16-20.
Zeng M S, Wu G Y, Wang Q S.Research progress on Myllocerinus aurolineatus Voss in China[J]. Journal of Henan University of Science and Technology (Agricultural Science), 2003, 23(4): 16-20.
[2] 张汉鹄, 谭济才. 中国茶树害虫及其无公害治理[M]. 合肥: 安徽科学技术出版社, 2004: 234-235.
Zhang H G, Tan J C.Chinese tea tree pests and their population-free treatment [M]. Hefei: Anhui Science and Technology Press, 2004: 234-235.
[3] 中国农业科学院植物保护研究所, 中国植物保护学会. 中国农作物病虫害[M]. 3版. 北京: 中国农业出版社, 2015: 88-91.
China society of plant protection, Institute of plant protection, Chinese academy of agricultural sciences. Diseases and pests affecting chinese crops [M]. 3rd ed. Beijing: China Agriculture Press, 2015: 88-91.
[4] 吴光远, 林阿祥, 郭剑雄. 巴丹防治茶丽纹象甲丽纹象甲的效果初报[J]. 茶叶科学技术, 1995(2): 17-18, 44.
Wu G Y, Lin A X, Guo J X.A preliminary report on the efficacy of cartap against Myllocerinus aurolineatus[J]. Tea Science and Technology, 1995(2): 17-18, 44.
[5] 王庆森, 曾明森. 应用锐劲特防治茶丽纹象甲的效果试验[J]. 福建茶叶, 2002(3): 6-7.
Wang Q S, Zeng M S.Experiment on the efficiency of using Ruijingte to prevent and control tea stripe weevil(Bagous sp.)[J]. Tea in Fujian, 2002(3): 6-7.
[6] 龙同, 乐群芬, 杨国琼, 等. 几种杀虫剂对茶丽纹象甲的室内、田间药效比较试验[J]. 湖北农业科学, 2019, 58(24): 109-112.
Long T, Yue Q F, Yang G Q, et al.The laboratory and field comparative effects of several pesticides against Myllocerinus aurolineatus[J]. Hubei Agricultural Sciences, 2019, 58(24): 109-112.
[7] Rehner S A, Minnis A M, Sung G H, et al.Phylogeny and systematics of the anamorphic, entomopathogenic genus Beauveria[J]. Mycologia, 2011, 103(5): 1055-1073.
[8] Bustamante D E, Oliva M, Leiva S, et al.Phylogeny and species delimitations in the entomopathogenic genus Beauveria(Hypocreales, Ascomycota), including the description of B. peruviensis sp. nov[J]. MycoKeys, 2019(58): 47-68.
[9] Chen W H, Liu M, Huang Z X, et al.Beauveria majiangensis, a new entomopathogenic fungus from Guizhou, China[J]. Phytotaxa, 2018, 333(2): 243-250.
[10] Chen W H, Han Y F, Liang Z Q, et al.A new araneogenous fungus in the genus Beauveria from Guizhou, China[J]. Phytotaxa, 2017, 302(1): 57-64.
[11] Kepler R M, Luangsa-ard J J, Hywel-Jones N L, et al. A phylogenetically-based nomenclature for Cordycipitaceae (Hypocreales)[J]. IMA Fungus, 2017, 8(2): 335-353.
[12] 蒲蛰龙, 李增智. 昆虫真菌学[M]. 合肥: 安徽科学技术出版社, 1996: 301-302.
Pu Z L, Li Z Z.Insect mycology [M]. Hefei: Anhui Science and Technology Press, 1996: 301-302.
[13] de Faria M R, Wraight S P. Mycoinsecticides and Mycoacaricides: A comprehensive list with worldwide coverage and international classification of formulation types[J]. Biology Control, 2007, 43(3): 237-256.
[14] 王定锋, 黎健龙, 李慧玲, 等. 茶丽纹象甲白僵菌广东分离株的鉴定及生物学特性研究[J]. 茶叶科学, 2015, 35(5): 449-457.
Wang D F, Li J L, Li H L, et al.Identification and biology characteristics research of a Myllocerinus aurolineatus-Beauveria strain isolated from Guangdong province[J]. Journal of Tea Science, 2015, 35(5): 449-457.
[15] Innis M A, Gelfand D H, Sninsky J J, et al.PCR protocols: a guide to methods and applications [M]. San Diego: Academic Press, 1990: 315-322.
[16] Rehner S A, Posada F, Buckley E P, et al.Phylogenetic origins of African and Neotropical Beauveria bassianas.l. pathogens of the coffee berry borer, Hypothenemus hampei[J]. Journal of Invertebrate Pathology, 2006, 93(1): 11-21.
[17] Tamura K, Dudley J, Nei M, et al.MEGA4: molecular evolutionary genetics analysis (MEGA) software version 4.0[J]. Molecular Biology and Evolution, 2007, 24(8): 1596-1599.
[18] 刘玉军, 张龙娃, 何亚琼, 等. 栎旋木柄天牛高毒力球孢白僵菌菌株的筛选[J]. 昆虫学报, 2008, 51(2): 143-149.
Liu Y J, Zhang L W, He Y Q, et al.Screening of high virulent strains of Beauveria bassiana against a dangerous forest cerambycid beetle, Aphrodisium sauteri (Coleoptera: Cerambycidae)[J]. Acta Entomologica Sinica, 2008, 51(2): 143-149.
[19] 王定锋, 李良德, 黎健龙, 等. 茶角胸叶甲高毒力球孢白僵菌菌株的筛选[J]. 茶叶科学, 2017, 37(3): 229-236.
Wang D F, Li L D, Li J L, et al.Screening of the Beauveria bassiana strain with high virulence to Basilepta melanopus (Coleoptera: Chrysomeloidea)[J]. Journal of Tea Science, 2017, 37(3): 229-236.
[20] 张建伟, 王中康, 申剑飞, 等. 小菜蛾高致病力绿僵菌的筛选、鉴定及培养特性研究[J]. 中国生物防治学报, 2012, 28(1): 53-61.
Zhang J W, Wang Z K, Shen J F, et al.Screening, identification and culture characteristics of a highly pathogenic strain of Metarhizium against Plutella xylostella[J]. Chinese Journal of Biological Control, 2012, 28(1): 53-61.
[21] 许齐爱, 李佳颖, 任顺祥. 红火蚁高致病力黄绿绿僵菌菌株的筛选[J]. 中国生物防治学报, 2013, 29(3): 384-388.
Xu Q A, Li J Y, Ren S X.Selection of Metarhizium flavoviride isolates to control the red imported fire ant Solenopsis invicta[J]. Chinese Journal of Biological Control, 2013, 29(3): 384-388.
[22] 侯明明, 刘义豪, 王滨. 昆虫病原真菌对梨网蝽防治潜力的室内评测[J]. 中国生物防治学报, 2015, 31(6): 853-859.
Hou M M, Liu Y H, Wang B.Laboratory assessment on virulence of entomogenous fungi against Stephanitis nashi[J]. Chinese Journal of Biological Control, 2015, 31(6): 853-859.
[23] Poprawski T J, Marchal M, Robert P H.Comparative susceptibility of Otiorhynchus sulcatus and Sitona lineatus(Coleoptera: Curculionidae) early stage to five entomopathogenic hyphomycetes[J]. Environment Entomology, 1985(14): 247-253.
[24] Soares G G, Marchal M, Ferron P.Susceptibility of Otiorhynchus sulcatus(Coleoptera: Curculionidae) larvae to Metarhizium anisopliae and Metarhizium flavoviridae, 1983(12): 1886-1890.
[25] 叶利芹, 吴小芹, 叶建仁. 竹叶锈病重寄生现象及重寄生菌鉴定[J]. 菌物学报, 2011, 30(3): 414-420.
Ye L Q, Wu X Q, Ye J R.Hyperparasitism of bamboo leaf rust and identification of the mycoparasite[J]. Mycosystema, 2011, 30(3): 414-420.
[26] 林华峰, 王萍莉, 张磊, 等. 布氏白僵菌和金龟子绿僵菌两变种的生长性状及其对蛴螬的毒力测定[J]. 中国生物防治, 2006, 22(2): 123-127.
Lin H F, Wang P L, Zhang L, et al.Growth of Beauveria brongniartii and two varieties of Metarhizium anisopliae and their virulence against white grubs[J]. Chinese Journal of Biological Control, 2006, 22(2): 123-127.
[27] Fargues J, Mania N K, Delmas J C, et al.Influence of temperature on the in vitro growth of entomopathogenic hyphomycetes[J]. Agronomie, 1992, 12(7): 557-564.
[28] Butt T M, Jachson C, Magan N.Fungi as biocontrol agents: progress, problems and potential[M]. UK: CABI, 2001: 23-69.
[29] Roberts D, St Leger R.Metarhizium spp., cosmopolitan insect-pathogenic fungi: mycological aspects[J]. Advances in Applied Microbiology, 2004, 54: 1-70.
[30] Rangel D E, Braga G U, Flint S D, et al.Variations in UV-B tolerance and germination speed of Metarhizium anisopliae conidia produced on insects and artificial substrates[J]. Journal of Insect Physiology, 2004, 87(2/3): 77-83.
[31] Hunt V L, Charnley A K.The inhibitory effect of the fungal toxin, destruxin A, on behavioural fever in the desert locust[J]. Journal of Insect Physiology, 2011, 57(10): 1341-1346.

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