Identification and Expression Analysis of Glucose-6-hosphate Dehydrogenase Gene (CsG6PDHs) in Camellia sinensis

WANG Yanding; WANG Huan; LI Nana; WANG Lu; HAO Xinyuan; WANG Yuchun; DING Changqing; YANG Yajun; WANG Xinchao; QIAN Wenjun

doi:10.13305/j.cnki.jts.20210622.002

Journal of Tea Science >

2021 , Vol. 41 >Issue 4: 497 - 510

DOI: https://doi.org/10.13305/j.cnki.jts.20210622.002

Research Paper

Identification and Expression Analysis of Glucose-6-hosphate Dehydrogenase Gene (CsG6PDHs) in Camellia sinensis

WANG Yanding ,
WANG Huan ,
LI Nana ,
WANG Lu ,
HAO Xinyuan ,
WANG Yuchun ,
DING Changqing ,
YANG Yajun ,
WANG Xinchao ,
QIAN Wenjun

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1. College of Horticulture, Qingdao Agricultural University, Qingdao 266109, China;
2. Tea Research Institute of the Chinese Academy of Agricultural Sciences/National Center for Tea Improvement/Key Laboratory of Tea Biology and Resources Utilization, Ministry of Agriculture and Rural Affairs, Hangzhou 310008, China;
3. College of Agriculture and Food Science, Zhejiang A&F University, Hangzhou 311300, China;

Received date: 2020-08-10

Revised date: 2020-09-23

Online published: 2021-08-12

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Abstract

As a key rate-limiting enzyme in pentose phosphate pathway, glucose-6-phosphate dehydrogenase (G6PDH, EC1.1.1.49) plays an important role in plant growth and development, and also in responding to environmental stresses. However, the function of G6PDH in tea plants has not yet been reported. In our study, 3 G6PDH genes were cloned from tea plant, named as CsG6PDH1 (MW025829), CsG6PDH2 (MW025830) and CsG6PDH4 (MW025831), respectively. Phylogenetic analysis shows that CsG6PDH1 and CsG6PDH4 belong to plastid protein, while CsG6PDH2 belongs to cytoplasmic protein. Quantitative analysis shows that the expressions of CsG6PDHs varied in different tissues. Under cold or Colletotrichum infection treatments, the time course expressions of both CsG6PDH1 and CsG6PDH4 were gradually reduced. Besides, both CsG6PDH1 and CsG6PDH4 were induced by cold acclimation in different tea cultivars. In addition, the expressions of CsG6PDHs were up-regulated during bud dormancy and flush periods. Consequently, our results implicate that CsG6PDHs are widely participated in tea plant growth and development, and also involved in responding to abiotic and biotic stresses. This study provided a theoretical basis for in-depth study of the function of CsG6PDHs in tea plants.

Key words： glucose-6-phosphate dehydrogenase; Camellia sinensis; expression analysis; cold; cold acclimation

Cite this article

WANG Yanding , WANG Huan , LI Nana , WANG Lu , HAO Xinyuan , WANG Yuchun , DING Changqing , YANG Yajun , WANG Xinchao , QIAN Wenjun . Identification and Expression Analysis of Glucose-6-hosphate Dehydrogenase Gene (CsG6PDHs) in Camellia sinensis[J]. Journal of Tea Science, 2021 , 41(4) : 497 -510 . DOI: 10.13305/j.cnki.jts.20210622.002

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