Study on the Regulation Roles of Plant Hormones on the Growth and Development of Tea Shoots in Spring

LI Congcong; WANG Haoqian; YE Yufan; CHEN Yao; REN Hengze; LI Yuteng; HAO Xinyuan; WANG Xinchao; CAO Hongli; YUE Chuan

doi:10.13305/j.cnki.jts.2023.03.002

Journal of Tea Science >

2023 , Vol. 43 >Issue 3: 335 - 348

DOI: https://doi.org/10.13305/j.cnki.jts.2023.03.002

Research Paper

Study on the Regulation Roles of Plant Hormones on the Growth and Development of Tea Shoots in Spring

LI Congcong ,
WANG Haoqian ,
YE Yufan ,
CHEN Yao ,
REN Hengze ,
LI Yuteng ,
HAO Xinyuan ,
WANG Xinchao ,
CAO Hongli ,
YUE Chuan

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1. Tea Research Institute, Chinese Academy of Agriculture Sciences/National Center for Tea Improvement/Key Laboratory of Biology, Genetics and breeding of Special Economic Animals and Plants, Ministry of Agriculture and Rural Affairs, Hangzhou 310008, China;
2. College of Horticulture, Fujian Agriculture and Forestry University, Fuzhou 350002, China;
3. College of Food Science, Southwest University, Chongqing 400715, China

Received date: 2023-02-15

Revised date: 2023-03-20

Online published: 2023-06-29

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Abstract

Hormones play important roles in the regulation of plant growth and development. In order to clarify the effects of different hormones on the growth and development of tea shoots in spring, and to identify the main pathways and key genes involved, tea cultivar ‘Longjing 43’ was used as the experimental materials, and treated with 100 μmol·L^-1 ABA, 100 μmol·L^-1 GA₃ and 100 μmol·L^-1 IAA respectively in the sprouting period. The phenotypic characteristics of buds were determined, and the buds at the 7th day after treatments were investigated using RNA-Seq technique. The results show that exogenous ABA treatment inhibited the germination and growth of shoots, and the length of shoots was significantly shorter than the control after 7 days after treatment. On the other hand, GA₃ and IAA treatments had a promoting effect, and the bud length was significantly extended on the 7th day after GA₃treatment and on the 14th day after IAA treatment. RNA-Seq analysis indicates differentially expressed genes were mainly enriched in amino acid biosynthesis pathway under ABA treatment, oxidative phosphorylation pathway and photosynthesis pathway under GA₃ treatment, and flavonoid biosynthesis pathway under IAA treatment. GAI, PSBO2, PSBQ-2 and PSBP-1 related to plant hormone and photosynthesis pathways might be the key genes involved in shoot growth and development. The real-time fluorescence quantitative PCR results of some candidate genes were consistent with the RNA-Seq results. The above studies identified the main pathways and key genes involved in the hormone regulation on tea shoot growth and development, which provided a theoretical basis for deeply revealing the regulation mechanism of tea shoot growth and development.

Key words： Camellia sinensis; ABA; GA₃; IAA; RNA-Seq; growth and development

Cite this article

LI Congcong , WANG Haoqian , YE Yufan , CHEN Yao , REN Hengze , LI Yuteng , HAO Xinyuan , WANG Xinchao , CAO Hongli , YUE Chuan . Study on the Regulation Roles of Plant Hormones on the Growth and Development of Tea Shoots in Spring[J]. Journal of Tea Science, 2023 , 43(3) : 335 -348 . DOI: 10.13305/j.cnki.jts.2023.03.002

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