Effect of Tea Polyphenols on the Determination of Reducing Sugar in Tea Food

SHENG Zheng; DU Wenkai; WANG Chongchong; ZHANG Boan; ZHANG Haihua; DU Qizhen

doi:10.13305/j.cnki.jts.2023.04.011

Journal of Tea Science >

2023 , Vol. 43 >Issue 4: 567 - 575

DOI: https://doi.org/10.13305/j.cnki.jts.2023.04.011

Research Paper

Effect of Tea Polyphenols on the Determination of Reducing Sugar in Tea Food

SHENG Zheng ,
DU Wenkai ,
WANG Chongchong ,
ZHANG Boan ,
ZHANG Haihua ,
DU Qizhen

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1. College of Food and Health, Zhejiang Agriculture and Forestry University, Hangzhou 311300, China;
2. Zhejiang Quality Inspection Center of Grain and Oil Products, Hangzhou 310012, China

Received date: 2023-03-02

Revised date: 2023-05-11

Online published: 2023-08-24

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Abstract

In order to find an accurate method to determine the content of reducing sugar in the digestive products of tea noodle products, phenolic acid, phenolic and glucose blending systems and phenolic and amylenzymeate blending systems alone were used to study the effects of four typical phenols including epigallocatechin gallate (EGCG), gallic acid, proanthocyanidin and ferulic acid on the quantitative determination of reducing sugars by 3,5-dinitrosalicylic acid (DNS) method, glucose oxidase/peroxidase (GOPOD) method and fluorescence-assisted sugar electrophoresis (FACE) method. The results show that ferulic acid had no effect on the DNS method, while EGCG, gallic acid and proanthocyanidin could react with DNS, indicating that they would affect the accuracy of the DNS method. All four phenolic substances significantly reduced the glucose results determined by GOPOD method, while the FACE method was not affected by phenols and could visually characterize the distribution of oligo reducing sugar in the amylase hydrolysate. Therefore, the FACE method has a good application value in determining the content of reducing sugars in tea noodle products and their enzymatic digestion products.

Key words： tea food; tea polyphenols; EGCG; reducing sugar content; fluorescence-assisted sugar electrophoresis

Cite this article

SHENG Zheng , DU Wenkai , WANG Chongchong , ZHANG Boan , ZHANG Haihua , DU Qizhen . Effect of Tea Polyphenols on the Determination of Reducing Sugar in Tea Food[J]. Journal of Tea Science, 2023 , 43(4) : 567 -575 . DOI: 10.13305/j.cnki.jts.2023.04.011

References

[1] Zhang Z Y, Liu C W, Fang W W, et al.Research progress on the lipid-lowering and weight loss effects of tea and the mechanism of its functional components[J]. The Journal of Nutritional Biochemistry, 2023, 112: 109210. doi: 10.1016/j.jnutbio.2022.109210.
[2] Jalaludin I, Kim J.Comparison of ultraviolet and refractive index detections in the HPLC analysis of sugars[J]. Food Chemistry, 2021, 365: 130514. doi: 10.1016/j.foodchem.2021.130514.
[3] Fang J, Sureda A, Silva A S, et al.Trends of tea in cardiovascular health and disease: a critical review[J]. Trends in Food Science & Technology, 2019, 88: 385-396. doi: 10.1016/j.tifs.2019.04.001.
[4] Yang C S, Wang H, Sheridan Z P.Studies on prevention of obesity, metabolic syndrome, diabetes, cardiovascular diseases and cancer by tea[J]. Journal of Food and Drug Analysis, 2018, 26(1): 1-13.
[5] 张士康. 茶面制品的产业前景[J]. 茶博览, 2018(2): 76-77.
Zhang S K.The industrial prospects of tea noodle products[J]. Tea Times, 2018(2): 76-77
[6] Lai W F, Baig M, Wong W T, et al.Epigallocatechin-3-gallate in functional food development: from concept to reality[J]. Trends in Food Science & Technology, 2020, 102: 271-279. doi: 10.1016/j.tifs.2020.04.001.
[7] Salelign K, Duraisamy R.Sugar and ethanol production potential of sweet potato (Ipomoea batatas) as an alternative energy feedstock: processing and physicochemical characterizations[J]. Heliyon, 2021, 7(11): e08402. doi: 10.1016/j.heliyon.2021.e08402.
[8] 张雅琦, 阮长青, 张东杰, 等. 抗性淀粉的分析方法研究进展[J]. 中国粮油学报, 2023, 38(3): 188-195.
Zhang Y Q, Ruan C Q, Zhang D J, et al.Research progress on analysis methods of resistant starch[J]. Journal of the Chinese Cereals and Oils Association, 2023, 38(3): 188-195.
[9] 曾文治, 雷郑延, 周欢, 等. 基于α-淀粉酶活性抑制率的茉莉花茶拼配研究[J]. 食品工业, 2019, 40(1): 163-168.
Zeng W Z, Lei Z Y, Zhou H, et al.Study on optimization of jasmine tea blending process for inhibiting α-amylase activity[J]. The Food Industry, 2019, 40(1): 163-168.
[10] 杨眷俪, 许雪蓉, 张振军, 等. 文冠果芽茶与叶茶主要营养功能成分分析及抗氧化活性评价[J]. 食品工业科技, 2022, 43(15): 366-373.
Yang J L, Xu X R, Zhang Z J, et al.Main nutritional and functional ingredients and antioxidant evaluation of Xanthoceras sorbifolia bunge bud tea and leaf tea[J]. Science and Technology of Food Industry, 2022, 43(15): 366-373.
[11] Zhu S, Liu B, Wang F, et al.Characterization and in vitro digestion properties of cassava starch and epigallocatechin-3-gallate (EGCG) blend[J]. LWT, 2021, 137: 110398. doi: 10.1016/j.lwt.2020.110398.
[12] 魏香玉, 于鲲, 吴迪, 等. 绿茶粉对高粱挂面品质及消化特性的影响[J]. 食品与发酵工业, 2022: 1-9[2023-03-02]. doi: 10.13995/j.cnki.11-1802/ts.032210.
Wei X Y, Yu K, Wu D, et al. Effect of green tea powder on the quality and digestibility of sorghum noodles [J]. Food and Fermentation Industries [J]. Food and Fermentation Industries, 2022: 1-9[2023-03-02]. doi: 10.13995/j.cnki.11-1802/ts.032210.
[13] Xu S Y, Kan J, Hu Z, et al.Quantification of neoagaro-oligosaccharide production through enzymatic hydrolysis and its anti-oxidant activities[J]. Molecules, 2018, 23(6): 1354. doi: 10.3390/molecules23061354
[14] Kraithong S, Teerapattarakan N, Balasubramanian B, et al.Bioactive compounds in tea: effect of imbalanced intake on digestive enzymes activity, cytochrome inhibition and drug interaction[J]. South African Journal of Botany, 2022, 150: 58-68.
[15] Cianciosi D, Forbes-Hernández T Y, Regolo L, et al. The reciprocal interaction between polyphenols and other dietary compounds: impact on bioavailability, antioxidant capacity and other physico-chemical and nutritional parameters[J]. Food Chemistry, 2022, 375: 131904. doi: 10.1016/j.sajb.2022.07.003.
[16] 郭有辉, 周娟, 胡露, 等. DNS光度法测定风味鱿鱼中淀粉、还原糖和蔗糖的含量[J]. 理化检验(化学分册), 2019, 55(4): 463-466.
Guo Y H, Zhou J, Hu L, et al.Determination of starch, reduceing sugar and sucrose in flavored squid by DNS photometric method[J]. Physical Testing and Chemical Analysis (Part B: Chemical Analysis), 2019, 55(4): 463-466.
[17] Jackson P.High-resolution polyacrylamide gel electrophoresis of fluorophore-labeled reducing saccharides[J]. Methods in Enzymology, 1994, 230: 250-265. doi: 10.1016/0076-6879(94)30017-8.
[18] Platzer M, Kiese S, Tybussek T, et al.Radical scavenging mechanisms of phenolic compounds: a quantitative structure-property relationship (QSPR) study[J]. Frontiers in Nutrition, 2022, 9: 882458. doi: 10.3389/fnut.2022.882458.
[19] Olszowy M.What is responsible for antioxidant properties of polyphenolic compounds from plants?[J]. Plant Physiology and Biochemistry, 2019, 144: 135-143.
[20] Lim J, Zhang X W, Ferruzzi M G, et al.Starch digested product analysis by HPAEC reveals structural specificity of flavonoids in the inhibition of mammalian α-amylase and α-glucosidases[J]. Food Chemistry, 2019, 288: 413-421.
[21] Yang B, Chen F, Hua Y L, et al.Prooxidant activities of quercetin, p-courmaric acid and their derivatives analysed by quantitative structure-activity relationship[J]. Food Chemistry, 2012, 131(2): 508-512.
[22] 李兵, 肖香兰, 杨永红, 等. 国内外淀粉含量的检测标准现状及检测方法研究进展[J]. 食品安全质量检测学报, 2021, 12(9): 3830-3839.
Li B, Xiao X L, Yang Y H, et al.Current situation of standard methods and research progress of starch content determination at China and abroad[J]. Journal of Food Safety & Quality, 2021, 12(9): 3830-3839.
[23] Tama A, Bartosz G, Sadowska-Bartosz I.Is hydrogen peroxide generated in wine?[J]. Food Bioscience, 2022, 45: 101487. doi: 10.1016/j.fbio.2021.101487.
[24] Khan M R, Volpe S, Salucci E, et al.Active caseinate/guar gum films incorporated with gallic acid: physicochemical properties and release kinetics[J]. Journal of Food Engineering, 2022, 335: 111190. doi: 10.1016/j.jfoodeng.2022.111190.
[25] O’Shea M G, Samuel M S, Konik C M, et al. Fluorophore-assisted carbohydrate electrophoresis (FACE) of oligosaccharides: efficiency of labelling and high-resolution separation[J]. Carbohydrate Research, 1998, 307(1): 1-12.

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