Study on the Effects of Puerins &#x02164;-&#x02166; on Four Kinds of Breast Cancer Cells

YANG Rui; ZHAO Xingping; HE Mingjie; LIU Min; LUO Rong; CHEN Chuanlong; PAN Shukang; DING Zhanggui

doi:10.13305/j.cnki.jts.2024.03.004

Journal of Tea Science >

2024 , Vol. 44 >Issue 3: 501 - 514

DOI: https://doi.org/10.13305/j.cnki.jts.2024.03.004

Research Paper

Study on the Effects of Puerins Ⅴ-Ⅶ on Four Kinds of Breast Cancer Cells

YANG Rui ,
ZHAO Xingping ,
HE Mingjie ,
LIU Min ,
LUO Rong ,
CHEN Chuanlong ,
PAN Shukang ,
DING Zhanggui

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1. Yunnan TAETEA Microbial Technology Co., Ltd., Kunming 650217, China;
2. Fermentation Engineering Research Center for Yunnan Pu-erh Tea, Kunming 650217, China;
3. Yunnan Institute of Microbiology, School of Life Sciences, Yunnan University, Kunming 650091, China;
4. Key Laboratory for Southwest Microbial Diversity of the Ministry of Education, Yunnan University, Kunming 650091, China;
5. Menghai Tea Industry Co., Ltd., TAETEA Group, Menghai 666200, China;
6. Key Laboratory of Pu-erh Tea Processing Technology, Ministry of Agriculture and Rural Affairs, Kunming 650217, China

Received date: 2024-01-03

Revised date: 2024-03-22

Online published: 2024-07-08

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Abstract

Puerins, a kind of nitrogen-containing polyphenol compound with unique structure, is a kind of catechin derivative first isolated from ripened Pu-erh tea. In this paper, the effects of puerins Ⅴ-Ⅶ on breast cancer cells were studied. Four breast cancer cell lines with different receptor phenotypes were used as models, and MTT assay, proteomics detection and apoptosis detection were carried out. The results show that all puerins Ⅴ, Ⅵ and Ⅶ had obvious inhibitory effects on the four kinds of breast cancer cells. Proteomics analysis indicates that the differentially expressed proteins were mainly concentrated in the metabolic pathway, followed by cancer pathway and adhesion spot signal pathway under the intervention of puerins Ⅴ-Ⅶ. Apoptosis detection demonstrates that all three puerins compounds could induce the apoptosis of MDA-MB-231 cells. This study first reported the anti-breast cancer activity and preliminary mechanism of puerins Ⅴ-Ⅶ, which provided basic information for studying the anti-cancer active substances of ripened Pu-erh tea.

Key words： ripened Pu-erh tea; puerins; breast cancer; proteomics; apoptosis

Cite this article

YANG Rui , ZHAO Xingping , HE Mingjie , LIU Min , LUO Rong , CHEN Chuanlong , PAN Shukang , DING Zhanggui . Study on the Effects of Puerins Ⅴ-Ⅶ on Four Kinds of Breast Cancer Cells[J]. Journal of Tea Science, 2024 , 44(3) : 501 -514 . DOI: 10.13305/j.cnki.jts.2024.03.004

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