Determination of Afidopyropen and Metabolite M440I007 in Tea Tissues by Modified QuEChERS Coupled with Ultra-high Performance Liquid Chromatography-Tandem Mass Spectrometry

QIN Yujie; GUO Mingming; CHEN Yongjing; ZHOU Li

doi:10.13305/j.cnki.jts.2024.03.010

2024 , Vol. 44 >Issue 3: 515 - 525

DOI: https://doi.org/10.13305/j.cnki.jts.2024.03.010

Research Paper

Determination of Afidopyropen and Metabolite M440I007 in Tea Tissues by Modified QuEChERS Coupled with Ultra-high Performance Liquid Chromatography-Tandem Mass Spectrometry

QIN Yujie ,
GUO Mingming ,
CHEN Yongjing ,
ZHOU Li

Expand

1. Research Center of Quality Safety for Agricultural Products, Tea Research Institute, Chinese Academy of Agricultural Sciences, Hangzhou 310008, China;
2. Graduate School of Chinese Academy of Agricultural Sciences, Beijing 100081, China;
3. Hengzhou Jasmine Industry Service Center, Hengzhou 530300, China

Received date: 2024-03-20

Revised date: 2024-04-26

Online published: 2024-07-08

Fold

Abstract

Afidopyropen, a novel biorational insecticide, was registered in China in 2023 for the control of tea leafhoppers in tea plantation. In this study, an analytical method was developed for the determination of afidopyropen and its metabolite M440I007 in tea tissues by a modified QuEChERS method combined with ultra-performance liquid chromatography-tandem mass spectrometry (UPLC-MS/MS). Roots, stems and leaves of tea plants were extracted by water and acetonitrile, and purified with primary secondary amine (PSA), hydroxylated multi-walled carbon nanotubes (MWCNT-OH) and graphitized carbon black (GCB). The target compounds were determined by UPLC-MS/MS in multiple reaction monitoring (MRM) mode, and quantified by external standard method with matrix-matched standard curve. Both targets display excellent linearity (R²>0.999 5) in the range of 0.002-5.000 mg·L^-1. The recoveries of both compounds at the spiked levels of 0.005-2.000 mg·kg^-1 ranged from 78.3% to 106.0% with the relative standard deviations (RSD) ≤ 12.85%. The limits of quantification (LOQs) and detection (LODs) of the method were 0.005 mg·kg^-1and 0.002 mg·kg^-1, respectively. The developed method was applied to detect afidopyrofen and M440I007 in tea seedling tissues cultured in soil treated with afidopyropen in a pot experiment for 7 days. The results show that the distribution of afidopyropen in the tissues was roots (0.102 mg·kg^-1) > stems (0.078 mg·kg^-1) > leaves (0.007 mg·kg^-1). The translocation factors, TF_root-stem and TF_stem-leaf, were both less than 1, and the residue levels of the metabolite M440I007 were below the LOQ. The method established in this study is low-cost, accurate and sensitive, which can serve as a technical basis for further research on the uptake and transport behaviors of afidopyropen and M440I007 in tea plants.

Key words： tea plant; pesticide residues; afidopyropen; M440I007; detection method

Cite this article

QIN Yujie , GUO Mingming , CHEN Yongjing , ZHOU Li . Determination of Afidopyropen and Metabolite M440I007 in Tea Tissues by Modified QuEChERS Coupled with Ultra-high Performance Liquid Chromatography-Tandem Mass Spectrometry[J]. Journal of Tea Science, 2024 , 44(3) : 515 -525 . DOI: 10.13305/j.cnki.jts.2024.03.010

References

[1] Affholder M C, Cohen G J V, Gombert-Courvoisier S, et al. Inter and intraspecific variability of dieldrin accumulation in Cucurbita fruits: new perspectives for food safety and phytomanagement of contaminated soils[J]. Science of the Total Environment, 2023, 859: 160152. doi: 10.1016/j.scitotenv.2022.160152.
[2] 谭海军. 新型生物源杀虫剂双丙环虫酯[J]. 世界农药, 2019, 41(2): 61-64.
Tan H J.The novel biological insecticide afidopyropen[J]. World Pesticides, 2019, 41(2): 61-64.
[3] 农业农村部农药检定所. 中国农药信息网[EB/OL]. [2024-3-2]. http://www.icama.org.cn/zwb/dataCenter.
Institute for the Control of Agrochemicals, Ministry of Agriculture and Rural Affairs. China pesticide information network [EB/OL]. [2024-3-2]. http://www.icama.org.cn/zwb/dataCenter.
[4] Guo M M, Sun H Z, Wang X R, et al.Residue behavior and risk assessment of afidopyropen and its metabolite M440I007 in tea[J]. Food Chemistry, 2023, 404: 134413. doi: 10.1016/j.foodchem.2022.134413.
[5] FAO and WHO. Pesticide residues in food2019-report 2019-joint FAO/WHO meeting on pesticide residues [EB/OL]. [2024-03-20]. https://www.fao.org/3/ ca7455en/ca7455en.pdf.
[6] Keigwin R P. Final registration decision for the new active ingredient; first new food & outdoor uses, afidopyropen [EB/OL]. (2018-09-04)[2024-03-20]. https://www.regulations.gov/document/EPA-HQ-OPP-2016-0416-0024.
[7] Shin J M, Choi S, Park Y H, et al.Comparison of QuEChERS and liquid-liquid extraction methods for the simultaneous analysis of pesticide residues using LC-MS/MS[J]. Food Control, 2022, 141: 109202. doi: 10.1016/j.foodcont.2022.109202.
[8] 马丽莎, 郑光明, 尹怡, 等. 固相萃取/超高效液相色谱-串联质谱法测定稻渔水体中氟虫腈及其代谢物[J]. 分析测试学报, 2022, 41(11): 1678-1683.
Ma L S, Zheng G M, Yin Y, et al.Determination of fipronil and its metabolites in rice fishing water samples by SPE/UPLC-MS/MS[J]. Journal of Instrumental Analysis, 2022, 41(11): 1678-1683.
[9] Zhang X Y, Li Z, Wang Y, et al.Preparation of black phosphorus nanosheets/zeoliticimidazolate framework nanocomposite for high-performance solid-phase microextraction of organophosphorus pesticides[J]. Journal of Chromatography A, 2023, 1708: 464339. doi: 10.1016/j.chroma.2023.464339.
[10] Bhuiya A, Yasmin S, Shaikh M A A, et al. Method development of multi pesticide residue analysis in country beans collected from Dhaka, Bangladesh, and their dietary risk assessment[J]. Food Chemistry, 2024, 445: 138741. doi: 10.1016/j.foodchem.2024.138741.
[11] 徐芷怡, 陈梦婷, 侯锡爱, 等. QuEChERS-高效液相色谱-串联质谱法同时测定芝麻油中7种农药残留[J]. 分析化学, 2020, 48(7): 928-936.
Xu Z Y, Chen M T, Hou X A, et al.Simultaneous determination of seven pesticide residues in sesame oil using QuEChERS-high performance liquid chromatography- tandem mass spectrometry[J]. Chinese Journal of Analytical Chemistry, 2020, 48(7): 928-936.
[12] 韩梅, 郭灵安, 焦颖, 等. 比较3种不同前处理方法结合高分辨质谱测定姜中农药残留的效果[J]. 食品安全质量检测学报, 2021, 12(9): 3674-3683.
Han M, Guo L A, Jiao Y, et al.Comparison of the effects of 3 kinds of different pretreatment methods combined with high-resolution mass spectrometry applied to the determination of pesticide residues in ginger[J]. Journal of Food Safety & Quality, 2021, 12(9): 3674-3683.
[13] 雷紫依, 苏光林, 李跑, 等. 植物源性食品中多农药残留GC-MS高通量快速检测技术研究进展[J]. 分析测试学报, 2023, 42(10): 1370-1380.
Lei Z Y, Su G L, Li P, et al.Research progress of high-throughput and rapid detection of multi-pesticide residues in plant-derived food samples based on GC-MS[J]. Journal of Instrumental Analysis, 2023, 42(10): 1370-1380.
[14] Schenck F J, Hobbs J E.Evaluation of the quick, easy, cheap, effective, rugged, and safe (QuEChERS) approach to pesticide residue analysis[J]. Bulletin of Environmental Contamination and Toxicology, 2004, 73(1): 24-30.
[15] Dong Y, Das S, Parsons J R, et al.Simultaneous detection of pesticides and pharmaceuticals in three types of bio-based fertilizers by an improved QuEChERS method coupled with UHPLC-q-ToF-MS/MS[J]. Journal of Hazardous Materials, 2023, 458: 131992. doi: 10.1016/j.jhazmat.2023.131992.
[16] 李自强, 杨梅, 张新忠, 等. 改良QuEChERS方法与UPLC-MS/MS联用测定茶叶中草甘膦、草铵膦及氨甲基膦酸[J]. 茶叶科学, 2023, 43(2): 263-274.
Li Z Q, Yang M, Zhang X Z, et al.Simultaneous determination of glyphosate, glufosinate and aminomethyl phosphonic acid residues in tea by modified QuEChERS method coupled with UPLC-MS/MS[J]. Journal of Tea Science, 2023, 43(2): 263-274.
[17] Chen K Y, Liu X G, Wu X H, et al.Simultaneous determination of afidopyropen and its metabolite in vegetables, fruit and soil using UHPLC-MS/MS[J]. Food Additives & Contaminants: Part A, 2018, 35(4): 716-723.
[18] 邵辉, 刘磊, 李辉, 等. UPLC-MS/MS法测定苹果中双丙环虫酯及其代谢物残留量[J]. 农药, 2019, 58(7): 511-514.
Shao H, Liu L, Li H, et al.Determination of afidopyropenandits metabolite residues in apple by UPLC-MS/MS[J]. Agrochemicals, 2019, 58(7): 511-514.
[19] Chawla S, Gor H N, Patel H K, et al.Validation of a QuEChERS-based method for the estimation of afidopyropen in Brinjal (Solanum melongena L.) and soil[J]. Journal of AOAC International, 2020, 103(1): 68-72.
[20] Xie J, Zheng Y X, Liu X G, et al.Human health safety studies of a new insecticide: dissipation kinetics and dietary risk assessment of afidopyropen and one of its metabolites in cucumber and nectarine[J]. Regulatory Toxicology and Pharmacology, 2019, 103: 150-157.
[21] Liu X L, Ban N, Fu Z X, et al.Persistent toxicity and dissipation dynamics of afidopyropen against the green peach aphid Myzus persicae (Sulzer) in cabbage and chili[J]. Ecotoxicology and Environmental Safety, 2023, 252: 114584. doi: 10.1016/j.ecoenv.2023.114584.
[22] 郇志博, 于世幸, 王明月, 等. 3种杀虫剂在豇豆中的残留行为及膳食风险评估[J]. 农药, 2023, 62(10): 741-745.
Huan Z B, Yu S X, Wang M Y, et al.Residue behavior and dietary risk assessment of 3 insecticides in cowpea[J]. Agrochemicals, 2023, 62(10): 741-745.
[23] Chen Y J, Guo M C, Liu X G, et al.Determination and dissipation of afidopyropen and its metabolite in wheat and soil using QuEChERS-UHPLC-MS/MS[J]. Journal of Separation Science, 2018, 41(7): 1674-1681.
[24] Hou X A, Qiao T, Zhao Y L, et al.Dissipation and safety evaluation of afidopyropen and its metabolite residues in supervised cotton field[J]. Ecotoxicology and Environmental Safety, 2019, 180: 227-233.
[25] Guo M M, Qin Y J, Sun H Z, et al.Method validation for detection of afidopyropen and M440I007 in tea[J]. Journal of the Science of Food and Agriculture, 2023, 103(12): 5738-5746.
[26] 邵奇, 吴涛, 解雪峰, 等. 不同植茶年限土壤氮素组分变化及其与环境因子关系[J]. 环境科学, 2024, 45(3): 1665-1673.
Shao Q, Wu T, Xie X F, et al.Changes in soil nitrogen components and their relationship with environmental factors with different tea plantation ages[J]. Environmental Science, 2023, 45(3): 1665-1673.
[27] Wang Z H, Wang X R, Wang M, et al.Establishment of a QuEChERS-UPLC-MS/MS method for simultaneously detecting tolfenpyrad and its metabolites in tea[J]. Agronomy, 2022, 12(10): 2324. doi: 10.3390/agronomy12102324.
[28] Perestrelo R, Silva P, Porto-Figueira P, et al.QuEChERS-fundamentals, relevant improvements, applications and future trends[J]. Analytica Chimica Acta, 2019, 1070: 1-28.
[29] 黄田田, 汤桦, 董晓倩, 等. 多壁碳纳米管QuEChERS-气相色谱法测定茶叶中23种有机磷农药残留量[J]. 食品科学, 2018, 39(6): 315-321.
Huang T T, Tang H, Dong X Q, et al.Determination of 23 organophosphorus pesticide residues in tea by QuEChERS extraction with multi-walled carbon nanotubes (MWCNTs) coupled to gas chromatography[J]. Food Science, 2018, 39(6): 315-321.
[30] 荣杰峰, 韦航, 李亦军, 等. 羟基化多壁碳纳米管分散固相萃取-气相色谱-质谱法测定茶叶中21种有机磷农药[J]. 色谱, 2016, 34(2): 194-201.
Rong J F, Wei H, Li Y J, et al.Determination of 21 organophosphorus pesticides in tea by gas chromatography-mass spectrometry coupled with hydroxylated multi-walled carbon nanotubes based on dispersive solid-phase extraction[J]. Chinese Journal of Chromatography, 2016, 34(2): 194-201.
[31] Zhu B Q, Xu X Y, Luo J W, et al.Simultaneous determination of 131 pesticides in tea by on-line GPC-GC-MS/MS using graphitized multi-walled carbon nanotubes as dispersive solid phase extraction sorbent[J]. Food Chemistry, 2019, 276: 202-208.
[32] Fang N, Zhao X P, Li Y J, et al.Uptake, translocation and subcellular distribution of broflanilide, afidopyropen, and flupyradifurone in mustard (Brassica juncea)[J]. Journal of Hazardous Materials, 2023, 452: 131381. doi: 10.1016/j.jhazmat.2023.131381.

Options

Outlines

模态框（Modal）标题

Abstract

Cite this article

References