Seven pesticides, including dinotefuran, difenoconazole, tolfenpyrad, chlorfenapyr, bifenthrin, diafenthiuron and glyphosate are registered and widely used as chemical pesticides on tea plants in China. These pesticides are key monitoring objects for pesticide residue risk assessment in tea. In this study, gas chromatography and ultra-high performance liquid chromatography tandem mass spectrometry were used to monitor the leaching rates of seven pesticides during green tea brewing. The results show that the leaching rates of pesticides in tea infusion were related to water solubility and octanol/water partition coefficient. Dinotefuran and glyphosate were easily soluble in water, so their average leaching rates in tea infusion were higher than 70%. The water solubilities of the other five pesticides were lower, and their average leaching rates were less than 11.3%. There were significant differences in the leaching rates of different pesticides during the first and second infusions. With the exception of bifenthrin, the average leaching rates of other six pesticides of the first infusion were 0.89-2.55 times that of the second infusion. A risk assessment of green tea made from fresh leaves 10 days after pesticide spraying was performed. According to the toxicity and human exposure risk assessment of pesticides, the risk of human health impact from ingesting seven residual pesticides through drinking green tea soup was relatively low, and the cumulative hazard quotient of the seven pesticides was only 4.1%.
[1] 国家统计局. 中国统计年鉴2024[M]. 北京: 中国统计出版社, 2024.
National Bureau of Statistics. China Statistical Yearbook 2024 [M]. Beijing: China Statistics Press, 2024.
[2] 中华人民共和国国家卫生健康委员会, 中华人民共和国农业农村部, 国家市场监督管理总局. 食品安全国家标准食品中农药最大残留限量: GB 2763—2021[S]. 北京: 中国标准出版社, 2021.
National Health Commission of the People’s Republic of China, Ministry of Agriculture, Rural Affairs of the People’s Republic of China, State Administration of Market Supervision. National food safety standard-maximum residue limits for pesticides in food: GB 2763—2021 [S]. Beijing: Standards Press of China, 2021.
[3] 中华人民共和国国家卫生健康委员会, 中华人民共和国农业农村部, 国家市场监督管理总局. 食品安全国家标准食品中2,4-滴丁酸钠盐等112种农药最大残留限量: GB 2763.1—2022 [S]. 北京: 中国农业出版社, 2022.
National Health Commission of the People’s Republic of China, Ministry of Agriculture a, Rural Affairs of the People’s Republic of China, State Administration of Market Supervision. National food safety standard-maximum residue limits for 112 pesticides in food: GB 2763.1—2022 [S]. Beijing: China Agricultural Press of, 2021.
[4] 霍增辉, 柳畅, 张玫, 等. 农药最大残留限量标准差异对我国茶叶出口RCEP成员国的贸易效率影响研究—基于随机前沿引力模型[J]. 茶叶科学, 2024, 44(3): 526-542.
Huo Z H, Liu C, Zhang M, et al.Study on the impact of standards differences in pesticide maximum residue limits on the trade efficiency of RCEP members exporting tea from China: based on the stochastic frontier gravity model[J]. Journal of Tea Science, 2024, 44(3): 526-542.
[5] 刘新, 陈红平, 王国庆. 中国茶叶质量安全40年[J]. 中国茶叶, 2019, 41(12): 1-9.
Liu X, Chen H P, Wang G Q.40 years of tea quality and safety in China[J]. China Tea, 2019, 41(12): 1-9.
[6] Anoop Kumar Barooah, Monorama Borthakur, Jatindra Nath Kalita, 等. 茶叶中农药残留及其通过茶汤进入人体量的估算[J]. 茶叶科学, 2011, 31(5): 419-426.
Barooah A K, Borthakur M, Kalita J N, et al.Pesticide residues in tea and their intake assessment using brew factor[J]. Journal of Tea Science, 2011, 31(5): 419-426.
[7] Szternfeld P, Demoury C, Brian W, et al.Modelling the pesticide transfer during tea and herbal tea infusions by the identification of critical infusion parameters[J]. Food Chemistry, 2023, 429: 136893. doi:10.1016/j.foodchem.2023.
136893.
[8] Wang X, Zhou L, Zhang X, et al.Transfer of pesticide residue during tea brewing: Understanding the effects of pesticide's physico-chemical parameters on its transfer behavior[J]. Food Research International, 2019, 121: 776-784.
[9] Chen H, Pan M, Liu X, et al.Evaluation of transfer rates of multiple pesticides from green tea into infusion using water as pressurized liquid extraction solvent and ultra-performance liquid chromatography tandem mass spectrometry[J]. Food Chemistry, 2017, 216: 1-9.
[10] 陈宗懋, 罗逢健, 周利, 等. 茶叶中农药残留和污染物管控技术体系创建及应用[J]. 茶叶科学, 2021, 41(1): 1-6.
Chen Z M, Luo F J, Zhou L, et al.Innovation and application of control system for pesticide residues and contaminants in tea[J]. Journal of Tea Science, 2021, 41(1): 1-6.
[11] 中国农药信息网. 农药登记数据[EB/OL].(2024-08-01)[2024-07-23]. http://www.icama.org.cn/zwb/dataCenter.
China Pesticide Information Network. Pesticide registration data [EB/OL]. (2024-08-01)[2024-07-23]. http://www.icama.org.cn/zwb/dataCenter.
[12] 中华人民共和国国家质量监督检验检疫总局, 中国国家标准化管理委员会. 茶叶感官审评方法: GB/T 23776—2018[S]. 北京: 中国标准出版社, 2018.
General Administration of Quality Supervision, Inspection and Quarantine of the People's Republic of China, Standardization Administration of the People's Republic of China. Methodology for sensory evaluation of tea: GB/T 23776—2018 [S]. Beijing: Standards Press of China, 2018.
[13] 诸力, 陈红平, 周苏娟, 等. 超高效液相色谱-串联质谱法测定不同茶叶中草甘膦、氨甲基膦酸及草铵膦的残留[J]. 分析化学, 2015, 43(2): 271-276.
Zhu L, Chen H P, Zhou S J, et al.Determination of glyphosate, aminomethyl phosphonic acid and glufosinate in different teas by ultra performance liquid chromatography-tandem mass spectrometry[J]. Chinese Journal of Analytical Chemistry, 2015, 43(2): 271-276.
[14] Wang M, Ning Y T, Hu Y, et al.Residue degradation and risk assessment of difenoconazole and its metabolite during tea growing, processing and brewing by ultra-performance liquid chromatography-tandem mass spectrometry determination[J]. Foods, 2024, 13(7): 1123. doi: 10.3390/foods13071123.
[15] 张新忠, 罗逢健, 刘光明, 等. 超高效液相色谱-串联质谱法测定茶叶和土壤中丁醚脲及其代谢物的残留[J]. 分析化学, 2011, 39(9): 1329-1335.
Zhang X Z, Luo F J, Liu G M, et al.Determination of diafenthiuron and its metabolites in tea and soil by ultra performance liquid chromatography-electrospray ionization tandem mass spectrometry[J]. Chinese Journal of Analytical Chemistry, 2011, 39(9): 1329-1335.
[16] 张芬, 张新忠, 陈宗懋, 等. 两种不同溶解度农药残留在茶汤中的浸出规律研究[J]. 茶叶科学, 2013, 33(5): 482-490.
Zhang F, Zhang X Z, Chen Z M, et al.Study on the brewing behavior of two kinds of different solubility pesticide from made tea to tea infusion[J]. Journal of Tea Science, 2013, 33(5): 482-490.
[17] Pesticide properties database (PPDB) [EB/OL].(2024-08-01)[2024-08-19]. https://sitem.herts.ac.uk/ aeru/
ppdb/en.
[18] 陈红平, 刘新, 鲁成银, 等. 茶叶内含物质与外源污染物在冲泡过程中的浸出规律[J]. 茶叶科学, 2020, 40(1): 63-76.
Chen H P, Liu X, Lu C Y, et al.Leaching pattern of internal substances and xenobiotic pollutants during tea brewing[J]. Journal of Tea Science, 2020, 40(1): 63-76.
[19] Chen H P, Pan M L, Pan R, et al.Transfer rates of 19 typical pesticides and the relationship with their physicochemical property[J]. Journal of Agricultural and Food Chemistry, 2015, 63: 723-730.
[20] Fang L, Long N, Li Y, et al.Transfer behavior of pesticides from honeysuckle into tea infusions: Establishment of an empirical model for transfer rate prediction[J]. Ecotoxicology and Environmental Safety, 2022, 234: 113377. doi: 10.1016/j.ecoenv.2022.113377.
[21] Gao W J, Yan M, Xiao Y, et al.Rinsing tea before brewing decreases pesticide residues in tea infusion[J]. Journal of Agricultural and Food Chemistry, 2019, 67(19): 5384-5393.
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