To study the effects and mechanism of (+)-catechins ((+)-C) on hypoxia pulmonary arterial hypertension (PAH) in rats, twenty seven SD male rats were randomly divided into 3 groups with equalnumber (control group, hypoxia group and hypoxia+(+)-C group). Mean pulmonary arterial pressure (mPAP), pulmonary vascular resistance (PVR) were measured to indicate the hemodynamic changes in vivo. After executing the rats, the right ventricle hypertrophy index (RVHI) and the vascular wall thickness of outer circumference ratio (WT%) were calculated. The expression of nitric oxide synthase (eNOS) and NO in pulmonary artery endothelial cells was analyzed by WB and NO kit respectively. Cellular viability of pulmonary arterial smooth muscle cell was determined by CCK-8 assay. The expression of calcium sensing receptor (CaSR) and total intracellular Ca2+ were analyzed by WB and fluorescent probe. The results showed that (+)-C could decrease the pulmonary arterial pressure induced by hypoxia (n=9, P＜0.05). Increase eNOS in pulmonary endothelial cells (n=3, P＜0.05) and increase NO synthesis in pulmonary endothelial cells (n=5, P＜0.05). The expression of CaSR in smooth muscle cells induced by hypoxia was inhibited (n=3, P＜0.05), while the calcium concentration was induced by (+)-C (n＝27, P＜0.05). Vascular smooth muscle cell proliferation was inhibited by (+)-C (n=30, P＜0.05). In conclusion, (+)-C can alleviate hypoxia induced PAH by inhibiting pulmonary vasoconstriction and vascular smooth muscle cell proliferation, providing a new approach for the treatment of PAH.
YAN Junjie
,
CHEN Fangzheng
,
CHEN Luowei
,
WANG Heng
,
HUANG Jingwen
,
JIN Lufei
,
XU Yuhui
,
YUAN Linbo
. The Effect and Mechanism of (+)-Catechins on Relieving Hypoxic Pulmonary Hypertension in Rats[J]. Journal of Tea Science, 2019
, 39(1)
: 55
-62
.
DOI: 10.13305/j.cnki.jts.2019.01.006
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