Taking 5 breeding lines tea as test material, the leaf functional and photosynthetic characteristics of the leaves at different positions were monitored, and correlations between the leaf functional and photosynthetic characteristic analyzed. The leaf functional characteristic included leaf area (LA), the leaf index(LI), specific leaf area (SLA), leaf dry matter content(LDMC), Chla, Chlb, Chl and Car, the photosynthetic indexes included net photosynthetic rate(Pn), stomatal conductance(Gs), intercellular CO2 concentration(Ci) and transpiration rate(Tr). The results showed that the LA, LMDC, Pn, Gs and Tr firstly increased and then decreased with the leaf position went up, whose maximum value emerged at the middle leaves(from 3rd to 4th). The 1 and 6 leaf measurements were substantially below other leaf position (P<0.05). The LI and SLA increased with the elevation of the leaf position, the maximum values of SLA was at 6th leaf (P<0.05). The Chla, Chlb and Chl of different position leaves (from 1st to 4th) were relatively high, and their amounts in leaves of the 6th leaf were usually lower than other leaf position (P<0.05). The variation trend of Car was not obvious with the leaf position went up. The correlation analysis indicated that the LA was significant and remarkably significant positive correlated with LI, LDMC, Chla, Chlb, Chl and Car, but significant negative with Ci, and not significant correlation with other indicators. LI had no significant correlation with other indicators (except LA). The SLA and LDMC, Chla, Chlb, Chl, Car were significant and remarkably significant negative correlation with Pn, but significant positive correlation with Ci, and not significant correlation with other indicators. The LDMC was significant and remarkably significant positive correlation with L Chla, Chl and Pn. The Chla, Chlb, Chl and Car were positively correlated with each other significantly, and the photosynthetic pigments were remarkably significant positive correlated with Pn. The Gs and Tr showed no significant correlation with other indicators. Our results suggest that: the LA, photosynthetic capacity and LDMC of middle leaves of (from 3rd to 4th) tea plants were relatively higher than those of other leaf positions, so the middle leaves should be the experimental material to test photosynthetic; SLA, LDMC and photosynthetic pigments were closely related with Pn, so they could be used as an index to evaluate the photosynthetic capacity.
[1] 宝乐, 刘艳红. 东灵山地区不同森林群落叶功能性状比较[J]. 生态学报, 2009, 29(7): 3692-3703.
[2] 宋彦涛, 周道玮, 王平, 等. 松嫩草地66种草本植物叶片性状特征[J]. 生态学报, 2013, 31(1): 79-88.
[3] Vogel S.Leaves in the lowest and highest winds: temperature, force and shape[J]. New Phytologist, 2009, 183(1): 13-26.
[4] Wright I J, Reich P B, Cornelissen J H C, et al. Modulation of leaf economic traits and trait relationships by climate[J]. Global Ecology and Biogeography, 2005, 14(5): 411-421.
[5] 姜元华, 许轲, 赵可, 等. 甬优系列籼粳杂交稻的冠层结构与光合特性[J]. 作物学报, 2014: 41(2): 286-296.
[6] Royer D L, Kooyman R M, Little S A, et al.Ecology of leaf teeth: a multi-site analysis from an Australian subtropical rainforest[J]. American Journal of Botany, 2009, 96(4): 738-750.
[7] 唐辉, 王满莲, 韦记青, 等. 林下与全光下地枫皮叶片形态和光合特性的比较[J]. 植物生理学通讯, 2010(9): 949-952.
[8] 郭春芳, 孙云, 陈常颂, 等. 茶树品种光合与水分利用特性比较及聚类分析[J]. 作物学报, 2008, 34(10): 1797-1804.
[9] 林郑和, 钟秋生, 陈常颂, 等. 缺氮条件下不同品种茶树叶片光合特性的变化[J]. 茶叶科学, 2013, 33(6): 500-504.
[10] 袁祖丽, 李华鑫, 吕立哲, 等. 豫南引种茶树品种光合特性与品质成分的研究[J]. 河南农业大学学报, 2010(3): 267-272.
[11] 金孝芳, 贾尚智, 石亚亚, 等. 不同绿茶品种(系)光合特性及叶绿素含量的比较研究[J]. 西南农业学报, 2013, 26(2): 520-523.
[12] 王桔红, 庄东红, 马瑞君, 等. 广东凤凰单枞茶树品种(系)叶片主要性状的种间差异和地域变异研究[J]. 茶叶科学, 2014, 34(5): 525-530.
[13] 闫小莉, 王德炉. 遮荫对苦丁茶树叶片特征及光合特性的影响[J]. 生态学报, 2014, 34(13): 3538-3547.
[14] 吴华玲, 李崇兴, 陈栋, 等. 基于形态鉴定及叶片解剖结构对云南白莺山古茶树资源的聚类分析[J]. 中国农学通报, 2014, 30(31): 114-121.
[15] Arnon D I.Copper enzymes in isolated chloroplasts. Polyphenoloxidase in Beta vulgaris[J]. Plant physiology, 1949, 24(1): 1-15.
[16] Vitasse Y, Lenz A, Kollas C, et al.Genetic vs. non-genetic responses of leaf morphology and growth to elevation in temperate tree species[J]. Functional ecology, 2014, 28(1): 243-252.
[17] 于鸿莹, 陈莹婷, 许振柱, 等. 内蒙古荒漠草原植物叶片功能性状关系及其经济谱分析[J]. 植物生态学报, 2014, 38(10): 1029-1040.
[18] Kiran T V, Rao Y V, Subrahmanyam D, et al.Variation in leaf photosynthetic characteristics in wild rice species[J]. Photosynthetica, 2013, 51(3): 350-358.
[19] 陆柳英, 曹升, 谢向誉, 等. 木薯叶形与光合特性、SPAD值的相关性研究[J]. 南方农业学报, 2014, 45(4): 558-564.
[20] 唐明德. 茶树新梢生育状况对叶片光合速率影响的研究[J]. 作物学报, 1987, 13(4): 336-344.
[21] 齐代华, 闵鹏, 贺丽, 等. 毛竹不同演替阶段四川山矾幼树比叶面积及叶干物质质量分数的研究[J]. 2015, 37(1): 1-7.
[22] Zhang L, Luo T.Advances in ecological studies on leaf lifespan and associated leaf traits[J]. Acta Phytoecological Sinica, 2003, 28(6): 844-852.
[23] 韩瑞锋, 李建明, 胡晓辉, 等. 甜瓜幼苗叶片光合变化特性[J]. 生态学报, 2012, 32(5): 1471-1480.
[24] 官玲亮, 吴卫, 郑有良, 等. 红花蕾期不同叶位叶的光合特性及与产量的相关性[J]. 作物学报, 2007, 33(8): 1352-1359.
[25] 黄晓敏. 闽南茶区不同乌龙茶品种叶片显微结构比较研究[D]. 福州: 福建农林大学, 2010: 20-38.
[26] 金松恒, 蒋德安, 王品美, 等. 水稻孕穗期不同叶位叶片的气体交换与叶绿素荧光特性[J]. 中国水稻科学, 2004, 18(5): 443-448.
[27] Zhang D Y, Wang X H, Chen Y, et al.Determinant of photosynthetic capacity in rice leaves under ambient air conditions[J]. Photosynthetica, 2005, 43(2): 273-276.
[28] Read Q D, Moorhead L C, Swenson N G, et al.Convergent effects of elevation on functional leaf traits within and among species[J]. Functional ecology, 2014, 28(1): 37-45.
[29] Coble A P, Cavaleri M A.Light drives vertical gradients of leaf morphology in a sugar maple (Acer saccharum) forest[J]. Tree physiology, 2014, 34(2): 146-158.
[30] 黄海侠, 杨晓东, 孙宝伟, 等. 浙江天童常绿植物当年生与往年生叶片性状的变异与关联[J]. 植物生态学报, 2013, 37(10): 912-921.
[31] 洪陈洁, 林晗, 洪伟, 等. 不同品系福建山樱花叶功能性状研究[J]. 热带亚热带植物学报, 2015, 23(2): 191-196.
[32] Garnier E, Shipley B, Roumet C, et al.A standardized protocol for the determination of specific leaf area and leaf dry matter content[J]. Functional ecology, 2001, 15(5): 688-695.
[33] Terashima I, Miyazawa S I, Hanba Y T.Why are sun leaves thicker than shade leaves?-Consideration based on analyses of CO2 diffusion in the leaf[J]. Journal of Plant Research, 2001, 114(1): 93-105.
[34] Wyka T P, Oleksyn J, Żytkowiak R, et al.Responses of leaf structure and photosynthetic properties to intra-canopy light gradients: a common garden test with four broadleaf deciduous angiosperm and seven evergreen conifer tree species[J]. Oecologia, 2012, 170(1): 11-24.
[35] 闫萌萌, 王铭伦, 王洪波, 等. 光质对花生幼苗叶片光合色素含量及光合特性的影响[J]. 应用生态学报, 2014, 25(2): 483-487.
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