It was assessed that the growth characteristics and succession rules of fungi involved in the fermentation of Fu brick tea during the winter and summer seasons in Hunan Province, China. 27 fungal strains were isolated and identified, and it was investigated that the growth and sporulation on different media, different carbon and nitrogen sources, and under different temperatures. The results showed that all fungi were able to grow on culture medium containing tea juice. Mycelium grew faster and were more prone to spore production in culture media with monosaccharides or disaccharides as the carbon source and organic nitrogen as the nitrogen source. The optimum growth temperature ranged at 25-30℃ for most fungi. The temperature higher than 30℃ or lower than 25℃ inhibited the growth of fungi. However, there were some differences between strains, it meant the different stain and succession rules of fungal strains during Fu brick tea fermentation in different seasons were closely related to the biological characteristics of fungi.
ZHAO Renliang
,
TAN Jihui
,
LU Qinhua
,
WU Dan
,
ZHU Qi
. Biological Characterization of Fungi Involved in Fu Brick Tea Fermentation[J]. Journal of Tea Science, 2016
, 36(2)
: 160
-168
.
DOI: 10.13305/j.cnki.jts.2016.02.006
[1] 傅冬和, 刘仲华, 黄建安, 等. 茯砖茶不同萃取物对消化酶活性的影响[J]. 茶叶科学, 2008, 28(1): 62-66.
[2] 黄群, 陈林杰, 李彦坡, 等. 冠突散囊菌黑茶发酵液对消化酶活性影响的研究[J]. 微生物学通报, 2007, 34(5): 917-920.
[3] 肖文军, 任国谱, 傅冬和, 等. 茯茶辅助调节血脂作用研究[J]. 茶叶科学, 2007, 27(3): 211-214.
[4] 施兆鹏. 茶叶加工学[M]. 北京: 中国农业出版社. 1997.
[5] 李珍, 雷雨, 苏晓倩, 等. 茯砖茶加工及保健功能研究进展[J]. 中国食物与营养, 2010(1): 40-42.
[6] 温琼英. 茯砖茶中主要微生物的研究[J]. 茶叶通讯, 1986(4): 19-21.
[7] 王志刚, 童哲, 程苏云. 茯砖茶中霉菌含量和散囊菌鉴定及利弊分析[J]. 食品科学, 1992(5): 1-5.
[8] 罗冰. 茯砖茶发酵菌生物学特性及其发酵剂制备研究[D]. 西安: 陕西科技大学, 2014.
[9] 丁婷. 茯砖茶中“金花菌”的生物学特性及其消化酶活性的研究[D]. 西安: 陕西科技大学, 2012.
[10] 丁婷, 吕嘉枥. 陕西茯砖茶中“金花”菌的生物学特性分析[J]. 食品工业. 2012, (1): 104-106.
[11] 邓庆森, 毛彦, 何梅珍, 等. 六堡茶中“金花”菌的生物学特性[J]. 中国茶叶加工, 2014(3): 19-24.
[12] Xu A, Wang Y, Wen J, et al.Fungal community associated with fermentation and storage of Fuzhuan brick-tea[J]. International Journal of Food Microbiology, 2011, 146: 14-22.
[13] Mo HZ, Zhang H, Li YQ, et al.Antimicrobial activity of the indigenously microbial fermented Fuzhuan brick-tea[J]. Journal of Biotechnology, 2008, 136: S722.
[14] 仓道平, 温琼英. 茯砖茶发酵中优势菌与有害菌类的分离鉴定[J]. 茶叶通讯, 1981(3): 12-14.