The volatile components of tea flower (Camellia sinensis var. assamica), which was collected from tea garden in Pu-erh district, were extracted by fully automated headspace solid-phase microextraction (HS-SPME), identified by gas chromatography-mass spectrometry (GC/MS), and compared with the volatile components of Pu-erh green tea from same tea garden. The results showed that 9 and 67 kinds of volatile components were identified in tea flower and Pu-erh raw tea, respectively. Among them, hydrocarbons and alcohols were two major volatile components. Comparison showed that all volatile components of the tea flower were also found in Pu-erh green tea. Our results suggests that the tea flower may have similar aroma to tea leaves, and can also be mixed with tea leaves to improve the aromatic flavor.
[1] 李辛雷, 李纪元, 范正琪, 等. 4种山茶花营养成分及有害元素含量分析[J]. 林业科学研究, 2010, 23(2): 298-301.
[2] 凌泽杰. 茶树花减肥降脂作用研究[D].杭州: 浙江大学, 2001.
[3] 庞式, 凌彩金. 茶树花开发利用的思路及其效益[J]. 广东茶业, 2002(增1): 39-40.
[4] 田围政. 茶树花营养成分的分析与评价[J]. 湖北民族学院学报: 自然科学版, 2004, 12(2): 26-28.
[5] 胡欢, 杨述章, 李仲芳, 等. 30种茶花过氧化物酶同工酶分析[J]. 四川师范大学学报: 自然科学版, 2013, 36(2): 296-301.
[6] Lv HP, Zhong QS, Lin Z, et al.Volatile characterisation of Pu-erh tea using headspace-solid phase microextraction combined with GC/MS and GC-olfactometry[J]. Food Chemistry, 2012, 130(4): 1074-1081.
[7] Ye NS, Zhang LQ, Gu XX.Discrimination of green teas from different geographical origins by using HS-SPME/ GC-MS and pattern recognition methods[J]. Food Analytical Methods, 2012, 5(4): 856-860.
[8] 洪涛, 黄遵锡, 李俊俊, 等. 普洱熟茶和生茶香气成分的提取和测定分析[J]. 茶叶科学, 2010, 30(5): 336-342.
[9] Lv SD, Wu YS, Song YZ, et al.Multivariate analysis based on GC-MS fingerprint and volatile composition for the quality evaluation of Pu-erh green tea[J]. Food Analytical Methods, 2015, 8(2):321-333.
[10] Du LP, Wang C, Li JX, et al.Optimization of headspace solid-phase microextraction coupled with gas chromatography-mass spectrometry for detecting methoxyphenolic compounds in Pu-erh tea[J]. Journal of Agricultural and Food Chemistry, 2013, 61(3): 561-568.
[11] Altaki M, Santos F, Galceran M, et al.Automated headspace solid-phase microextraction versus headspace for the analysis of furan in foods by gas chromatography-mass spectrometry[J]. Talanta, 2009, 78(4): 1315-1320.
[12] 吕世懂, 姜东华, 杨凡, 等. 顶空固相微萃取/GC-MS分析普洱熟茶与安化黑茶香气成分[J]. 热带作物学报, 2013, 34(8): 1583-1591.
[13] 朱旗, 施兆鹏, 童京汉, 等. GC-MS测定生茶及速溶生茶的游离脂肪酸[J]. 茶叶科学, 2001, 21(2): 137-139.
[14] Leffingwell JC, Alford ED, Leffingwell D.Volatile constituents of a supercritical CO2 extract of Kentucky dark fire-cured tobacco[J]. Leffingwell Reports, 2013, 5(1): 1-21.
[15] Lin J, Dai Y, Guo YN, et al.Volatile profile analysis and quality prediction of longjing tea (Camellia sinensis) by HS-SPME/GC-MS[J]. Journal of Zhejiang University, Science, B, 2012, 13(12): 972-980.
[16] Lv SD, Wu YS, Li CW, et al.Comparative analysis of Pu-erh and Fuzhuan teas by fully automatic headspace solid-phase microextraction coupled with gas chromatography-mass spectrometry and chemometric methods[J]. Journal of Agricultural and Food Chemistry, 2014, 62(8): 1810-1818.
[17] Wang KB, Liu F, Liu ZH, et al.Comparison of catechins and volatile compounds among different types of tea using high performance liquid chromatograph and gas chromatograph mass spectrometer[J]. International Journal of Food Science and Technology, 2011, 46(7): 1406-1412.
[18] Pang XL, Qin ZH, Zhao L, et al.Development of regression model to differentiate quality of black tea (Dianhong): correlate volatile properties with instrumental data using multiple linear regression analysis[J]. International Journal of Food Science and Technology, 2012, 47(11): 2372-2379.
[19] Lv SD, Wu YS, Zhou JS, et al.Analysis of volatile components of dark teas from five different production regions by fully automatic headspace solid-phase microextraction coupled with gas chromatography-mass spectrometry[J]. Journal of Chemical and Pharmaceutical Research, 2014, 6(1): 246-253.
[20] Wang LF, Lee JY, Chuang JO.Discrimination of teas with different degrees of fermentation by SPME-GC analysis of the characteristic volatile flavour compounds[J]. Food Chemistry, 2008, 109(1): 196-206.
[21] 罗发美, 詹家芬, 罗正刚, 等. SPME-GC-MS分析普洱茶的挥发性成分[J]. 林产化学与工业, 2010, 30(5): 95-98.
[22] 胡兴华, 王燕, 邹伶俐, 等. 茶花品种SSR指纹图谱分型技术反映体系优化[J]. 中国农学通报, 2013, 29(1): 127-131.
[23] 黄海涛, 陈章玉, 施红林. 茶叶香味扫描和挥发性化学成分分析[J]. 分析化学研究简报, 2005, 33(8): 1185-1188.
[24] 曹艳妮, 刘通讯. 不同储存时间普洱生茶和熟茶香气成分分析[J]. 食品工业, 2011(10): 64-67.
[25] 张峻松, 张常记, 郑峰洋, 等. 超高压处理对普洱生茶香味成分的影响研究[J]. 茶叶科学, 2008, 28(4): 267-272.
[26] Wang LF, Lee JY, Chun JO.Discrimination of teas with different degrees of fermentation by SPME-GC analysis of the characteristic volatile flavour compounds[J]. Food Chemistry, 2008, 109(1): 196-206.
[27] 杨明容, 马燕, 杨四润, 等. 气质联用分析云南老树茶香气成分[J]. 中国农学通报, 2012, 28(24): 261-269.
[28] 黄致喜, 王慧辰. 萜类香料化学[M]. 北京: 中国轻工业出版社, 1999: 397.
[29] 范正琪, 李纪元, 田敏, 等. 三个山茶花种(品种)香气成分初探[J]. 园艺学报, 2006, 33(3): 592-596.
[30] Lv SD, Wu YS, Wei J, et al.Application of gas chromatography-mass spectrometer and chemometrics methods for assessing volatile profiles of Pu-erh tea with different processing ways and ageing year[J]. Rsc Advances, 2015, 5: 87806-87817.
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