Tea green leafhopper, Empoasca vitis, is the most serious pest in tea gardens. The detoxifying enzyme activities and synergism in the bifenthrin-resistant and susceptible leafhopper were measured via biochemical methods and bioassays to clarify their potential relationship to the resistance of tea leafhopper to bifenthrin. The detoxifying enzyme activity tests showed that the activities of carboxylesterase (CarE), Cytochrome P450-mediated O-demethylation activity toward p-nitroanisole (PNOD) and glutathione-S-transferase (GST) in the resistant strain were all higher than those in susceptible strain. The activities of the three detoxifying enzymes in bifenthrin-resistant strain were 2.09, 4.34 and 1.59 folds those of the susceptible strain respectively. Synergism tests showed that the synergist ratios of triphenyl phosphate (TPP), piperonyl buoxide (PBO) and diethyl maleate (DEM) were 1.04, 1.09, and 1.00 folds respectively in the bifenthrin-susceptible strain and 1.80, 7.97 and 1.03 foldsrespectively in the bifenthrin-resistant strain. These results indicate that CarE and PNOD play important roles in bifenthrin resistance of tea leafhoppers.
LI Jianyu
,
SHI Mengzhu
,
FU Jianwei
,
WANG Ting
,
ZHANG Zhikang
. Activities of the Detoxifying Enzymes and Synergism in the Bifenthrin-resistant and Susceptible Strains of Tea Leafhopper, Empoasca vitis[J]. Journal of Tea Science, 2016
, 36(3)
: 323
-329
.
DOI: 10.13305/j.cnki.jts.2016.03.012
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