A model of age pigment generation was builted by carbonyl-ammonia cross linking reaction in vitro, the datas indicate that theaflavins can not only inhibit the generation of age pigment in protein carbonylation, but also supress β-sheet-rich in aggregate protein in this model by thioflavin T-based fluorometric assay, scanning electron microscope, transmission electric microscope, SDS-PAGE and NBT staining, there are postive relationship between protein carbonylation on mechanism of aging and protein aggregation that lead to neurodegenerative disease.
ZHANG Jing
,
CAI Shuxian
,
HUANG Jian'an
,
LI Juan
,
ZHONG Ni
,
YANG Heng
,
LIU Zhonghua
. Inhibition of Theaflavins on Protein Carbonylation and Aggregation in the System of BSA/MDA Carbonyl-ammonia Cross Linking Reaction in vitro[J]. Journal of Tea Science, 2016
, 36(4)
: 363
-371
.
DOI: 10.13305/j.cnki.jts.2016.04.004
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