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Journal of Tea Science ›› 2009, Vol. 29 ›› Issue (5): 336-340.doi: 10.13305/j.cnki.jts.2009.5.002

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Prokaryotic Expression of α-tubulin Gene of Camellia sinensis and Preparation of α-tubulin Polyclonal Antibody

TAN Zhen, TONG Xin, FANG Chao, JIANG Chang-jun*, CHEN Cong   

  1. Key Laboratory of Tea Biochemistry and Biotechnology, Ministry of Education, Anhui Agricultural University, Hefei 230036,China
  • Received:2009-02-07 Revised:2009-04-21 Online:2009-10-15 Published:2019-09-09

Abstract: According to the relevant complete mRNA sequence of α-tubulin gene of Camellia sinensis (accession NO. DQ340766), the coding region of α-tubulin gene was amplified using RT-PCR method, and its products were expressed in Escherichia coli using the pET-32a(+) vector. The fusion protein was expressed in the form of inclusion body when induced with isopropyl-D-thiogalatopyranoside (IPTG), then was purified to immunize the rabbit to produce the polyclonal antibody against α-tubulin. The specificity of the antibody was confirmed by Western blot analysis. The results of Western blot revealed well specificity of the antibody against α-tubulin in Camellia sinensis plant.

Key words: tea plant(Camellia sinensis), α-tubulin, prokaryotic expression, polyclonal antibody

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