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Journal of Tea Science ›› 2009, Vol. 29 ›› Issue (5): 379-384.doi: 10.13305/j.cnki.jts.2009.5.008

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Study on the Analytical Method of EGCG 3″Me by HPLC in Tea

SUN Ye-liang1,2, LV Hai-peng2, LIN Zhi2,*, LI Xing-hui1,*   

  1. 1.Tea Research Institute, Nanjing Agricultural University, Nanjing 210095, China;
    2. Engineering Research Center for Tea Processing; Tea Research Institute, Chinese Academy of Agricultural Sciences, Hangzhou 310008, China
  • Received:2009-03-23 Revised:2009-04-10 Published:2019-09-09

Abstract: EGCG3″Me was isolated by preparative HPLC system from tea and identified by NMR and LC/MS. The influence of different extracting method on the EGCG3″Me content in tea was discussed, and a simple high performance liquid chromatography analytical method of EGCG3″Me was developed. Results showed that the analytical method showed a good linear relationship between the peak area and content of EGCG3″Me (R2=0.9998) and high reproducibility. The average recovery rate of EGCG3″Me was 95.48% and the relative standard deviation(RSD)was 3.31%. The EGCG3″Me concentration in tea infusion can reach highest when the tea sample was immersed in distilled water for 5 min at 90℃.

Key words: tea, EGCG3"Me, analysis, content

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