茯砖茶及其配方对脂肪变性L-02肝细胞中TG含量的影响

doi:10.13305/j.cnki.jts.2011.03.011

Abstract

Abstract: Using 1mL/L of 20% medical fat emulsion to process L-02 liver cells for 48h in order to replicate non-alcoholic fatty degeneration of liver cells, and then adding different concentrations of extracts and its formulas to process separately for 24h and 48h, automatic biochemical analyzer detects the level of triglycerides (TG) in liver cells, investigate the effects of Fu Brick Tea extraction and the two formulas on the content of TG in L-02 Steatotic Hepatocytes. Results showed that Fu brick tea extraction and formula 1 can significantly lower TG levels in fatty liver cells(P<0.05), while comparing with the Xue Zhi Kang group, those two groups presented no significant difference (P>0.05). Accordingly, Fu brick tea and formula 1 reflect a wide prospect of development and application.

Key words: Fu brick tea, hepatocytes steatosis, L-02 hepatocyte, formula, triglyceride

CLC Number:

XU Xiao-jiang, HUANG Jian-an, XIAO Li-zheng, YUAN Yong, XIAO Wen-jun. Effects of Fu Brick Tea and Its Formula on the Content of TG in L-02 Steatotic Hepatocytes[J]. Journal of Tea Science, 2011, 31(3): 247-254.

References

[1] 肖文军, 任国谱, 傅冬和, 等. 茯茶辅助调节血脂作用研究[J]. 茶叶科学, 2007, 27(3): 211-214.
[2] 傅冬和, 刘仲华, 黄建安, 等. 通量筛选研究茯砖茶降脂功效[J]. 茶叶科学, 2006, 26(3): 209-214.
[3] 何德华, 詹镕洲. 肝胆病理学[M]. 上海: 第二军医大学出版社, 1997.
[4] 丁佳, 范竹萍. 非酒精性脂肪肝和心血管疾病[J]. 国际消化病杂志, 2008, 28(4): 320-322.
[5] Jin Hang-Bin, Zhu-Ying Gu, Yu Chao-Hui, et al. Association of nonalcoholic fatty liver disease with 2 diabetes: clinical features and independent risk factors in diabetic fatty liver patients[J]. Hepatobiliary Pancreat Dis Int, 2005, 4(3): 389-392.
[6] Shimada M, Hash E, Kaneda H, et al. Nonalcoholic for liver fibrosis[J]. Hepatology Research, 2002, 24(5): 345-347.
[7] 张华捷, 庄辉, 刘学恩. 脂肪肝的流行病学研究进展[J]. 中华流行病学杂志, 2004, 25(7): 630-632.
[8] 司徒镇强. 实用生物组织学技术: 第1版[M]. 北京: 科学出版社, 2004: 196-197.
[9] 欧健. 大黄素对脂肪变性L02肝细胞UCP2mRNA表达的影响[D]. 广州: 暨南大学, 2009.
[10] 胥国强, 蒲泽宴, 刘华, 等. 辛伐他汀通过Caspase-12活化途径诱导K562细胞凋亡的实验研究[J]. 四川大学学报: 医学版, 2008, 39(6): 900-904.
[11] 赵嘉惠, 张华屏, 王春芳. MTT法在检测细胞增殖方面的探讨[J]. 山西医科大学学报, 2007, 38(3): 262-263.
[12] 樊希承, 黄颖, 黄亦琦, 等. 脂肪肝肝脂与血脂的相关性研究[J]. 实用医技杂志, 2006, 13(20): 3531-3533.
[13] Day CP.Pathogenesis of steatohepatitis[J]. Best Pract Res Clin Gastroenterol, 2002, 16(7): 663-678.
[14] Sakagami H, Satoh K.Prooxidant: ascorbic acid and Gallic acid[J]. Anticancer Res, 1997, 17(1A): 221-224.
[15] 吕海鹏, 林智, 谷记平, 等. 普洱茶中的没食子酸研究[J]. 茶叶科学, 2007, 27(2): 104-110.
[16] 折改梅, 张香兰, 陈可可, 等. 茶氨酸和没食子酸在普洱茶中的含量变化[J]. 云南植物研究, 2005, 27(5): 572-576.
[17] 袁勇, 黄建安, 徐小江, 等. 茯茶中“金花”孢子粉提取物对体外诱导的非酒精性脂肪肝细胞内甘油三酯代谢的影响[J]. 茶叶科学, 2011, 31(2): 129-135.
[18] 熊金萍, 郭红卫, 顾雪峰. 山楂提取物对人体血脂影响的研究[J]. 中国公共卫生, 2004, 20(12): 1469-1470.
[19] Visavadiya NP, Narasimhacharya A.Hypocholesterolaemic and antioxidant effects of Glycyrrhiza glabra (Linn) in rats[J]. Mol Nutr Food Res, 2006, 50(11): 1080.
[20] 高雪岩, 王文全, 魏胜利, 等. 甘草及其活性成分的药理活性研究进展[J]. 中国中药杂志, 2009, 34(21): 2695-2700.

Effects of Fu Brick Tea and Its Formula on the Content of TG in L-02 Steatotic Hepatocytes

PDF (PC)

Knowledge

Cited

Abstract

Cite this article

share this article

References

Related Articles 13

Metrics

Comments

Recommended 10

[1]	DAI Xinyue, GE Binggang, ZHANG Xuwen, LIU Wenwu, DUAN Jichun, FU Donghe. Effect of Fu Brick Tea on Improving Metabolic Disorders in Type 2 Diabetes Mice [J]. Journal of Tea Science, 2022, 42(1): 63-75.
[2]	HUANG Xiangxiang, TAN Ting, YU Lijun, WANG Kunbo, HUANG Jian'an, XU Shiyu, LIU Zhonghua. Effects of Fu Brick Tea on Inflammation and Intestinal Microflora Diversity in Mice with DSS-induced Ulcerative Colitis [J]. Journal of Tea Science, 2021, 41(5): 681-694.
[3]	ZUO Gaolong, CHEN Meiyan, LIN Yong, LIN Haiyan, LIU Zhonghua, WU Wenliang. Protective Effect of Fu Brick Tea on Intestinal Mucosal Barrier and Liver Injury in NAFLD Rats [J]. Journal of Tea Science, 2019, 39(6): 631-640.
[4]	HU Ankai, YAO Liyun, ZHAO Yueling, LIU Chang, WANG Yuefei, XU Ping. Protective Effects of Fu Brick Tea Extracts on Inflammatory Bowel Disease Induced by Dextran Sulfate Sodium in Mice [J]. Journal of Tea Science, 2019, 39(6): 641-651.
[5]	HOU Aixiang, YAN Daomin, SUN Jingwen, ZHENG Xu, LI Saidan, XIAO Runhua, BEI Shuang. Effects of Green, Black and Fu Brick Tea Aqueous Extracts on the Characteristics of Intestinal Microbiota during in vitro Fermentation [J]. Journal of Tea Science, 2019, 39(4): 403-414.
[6]	LIN Qin, ZHOU Li, LUO Fengjian, YANG Jie, WANG Xinru, SUN Hezhi, YANG Mei, CHEN Zongmao. Determination of Alkylphenol Ethoxylates in Agrochemicals for Tea Garden by UPLC-MS/MS [J]. Journal of Tea Science, 2019, 39(1): 105-113.
[7]	WU Genliang, HOU Aixiang, LI Ke, LI Zongjun. Effects of Polyphenols of Old Fu Brick Tea on the Elderly Intestinal Flora [J]. Journal of Tea Science, 2018, 38(3): 319-330.
[8]	SONG Juan, WANG Dong, YU Xudong. Studies on the Preparation and Stability of Tea Polyphenol Emulgel Formulation [J]. Journal of Tea Science, 2017, 37(6): 623-630.
[9]	SHEN Chengwen, DENG Yuezhao, ZHOU Yuebin, QI Dongqing, HE Qun, TIAN Shuanghong, LIN Juan. Research of Quality Features and Aroma Components in Hunan Fu Brick Tea [J]. Journal of Tea Science, 2017, 37(1): 38-48.
[10]	ZHAO Renliang, TAN Jihui, LU Qinhua, WU Dan, ZHU Qi. Biological Characterization of Fungi Involved in Fu Brick Tea Fermentation [J]. Journal of Tea Science, 2016, 36(2): 160-168.
[11]	XIA Jiajie, XUE Meng, JIN Meng, WU Jueli, LIU Handeng. Preliminary Reserch of Tea Polyphenols on Insulin Resistance [J]. Journal of Tea Science, 2014, 34(2): 144-148.
[12]	LI Su, ZHU Nan-nan, LIU Zhong-hua, JIANG Hua-jun. Effect of Dark Tea on Fetal Bovine Serum Fat Accumulation in HepG2 Cells [J]. Journal of Tea Science, 2013, 33(1): 45-52.
[13]	MA Wei-guang, HUANG Zhi-pu, YOU Wen-long, LI Jin-shan, ZENG Guang-yuan, LIU Yu-qing. Research on the Acute Toxicity of Formulated Puer Tea [J]. Journal of Tea Science, 2010, 30(1): 9-12.