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Journal of Tea Science ›› 2019, Vol. 39 ›› Issue (1): 105-113.doi: 10.13305/j.cnki.jts.2019.01.012

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Determination of Alkylphenol Ethoxylates in Agrochemicals for Tea Garden by UPLC-MS/MS

LIN Qin1,2,3, ZHOU Li1,3,*, LUO Fengjian1,3, YANG Jie1,2,3, WANG Xinru1,3, SUN Hezhi1,3, YANG Mei1,3, CHEN Zongmao1,3,*   

  1. 1. Research Center of Quality Safety for Agricultural Products, Tea Research Institute, Chinese Academy of Agricultural Sciences, Hangzhou 310008, China;
    2. Graduate School of Chinese Academy of Agricultural Sciences, Beijing 100081, China;
    3. Key Laboratory of Tea Quality and Safety Control, Ministry of Agriculture, Hangzhou 310008, China
  • Received:2018-08-13 Revised:2018-10-08 Online:2019-02-15 Published:2019-07-17

Abstract: This study established an ultra high performance liquid chromatography-tandem mass spectrometry method for the determination of alkylphenol polyoxyethylene (APEOs) in pesticide formulations. APEOs were separated on a Waters-BEH C18 column, measured by a multi-reaction monitoring mode (MRM) with an electrospray positive ion source (ESI+), and quantified by an external standard method. The matrix standard curve of APEOs (n=3-20) in aqueous solutions, water emulsion, suspension concentrate, emulsifiable concentrate,granules and wettable powder had a good linearity in the range of 0.047-1β503.6βmg·kg-1with the correlation coefficient (r) between 0.996β1 and 0.999β9. The recovery of APEOs spiked in the level of 0.5-1β503.6βmg·kg-1 ranged from 83.8-120.4% with relative standard deviation (RSD) of 0.6-13.7%, and the limits of quantity (LOQs) of 0.5~150.4βmg·kg-1. Among the 51 pesticide formulations used in tea gardens, the percentage of Octylphenol ethoxylates (OPEOs) was less than 0.5%. The mass fraction of nonylphenol ethoxylates (NPEOs) was ND-32.1% and the detection rate was 90.2%, indicating the wide use of NPEOs in pesticides in tea gardens.

Key words: pesticide additives, pesticide formulations, tea, alkylphenol ethoxylates, ultra high performance liquid chromatography-tandem mass spectrometry

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