Infestation of Ectropis obliqua Enhances Neighboring Tea Plant Defenses Against Conspecific Larvae

LEI Shu; LI Xiwang; SUN Xiaoling; WANG Zhiying; XIN Zhaojun

doi:10.13305/j.cnki.jts.2016.06.005

PDF(761 KB)

Journal of Tea Science ›› 2016, Vol. 36 ›› Issue (6) : 587-593. DOI: 10.13305/j.cnki.jts.2016.06.005

Infestation of Ectropis obliqua Enhances Neighboring Tea Plant Defenses Against Conspecific Larvae

LEI Shu^1,2, LI Xiwang^2,3, SUN Xiaoling^2,3, WANG Zhiying^1,*, XIN Zhaojun^2,3,*

Author information +

History +

Abstract

In this study, the effect of herbivore-induced plant volatiles (HIPVs) from tea plant on the defense responses of neighboring tea plants was analyzed. The expression levels of defense-related genes, the content of defense compound in tea and the mass of Ectropis obliqua (TG) larvae were measured respectively. Compared with the control plants, the results showed that HIPVs released from TG-infested tea plants significantly induced the relative expression levels of CsiLOX1 and CsiACS1 in the neighboring tea plants within 24 h and 12 h. TG-induced expression of CsiLOX1 and CsiACS1 were also enhanced by HIPVs. Three days after HIPVs exposure, the polyphenol oxidases（PPOs） activity in the treated tea leaves was 1.36-fold higher than that of control. Meanwhile, the HIPVs-treated tea plants reduced the performance of TG. The above results showed that volatiles released from TG-infested tea can convey information to those neighboring plants and then trigger the induced defense responses against TG by modulating JA and ET signaling pathways in tea.

Key words

Ectropis obliqua / Camellia sinesis / induced defense response / neighboring tea plants

Cite this article

EndNote

Ris (Procite)

Bibtex

Download Citations

LEI Shu, LI Xiwang, SUN Xiaoling, WANG Zhiying, XIN Zhaojun. Infestation of Ectropis obliqua Enhances Neighboring Tea Plant Defenses Against Conspecific Larvae[J]. Journal of Tea Science. 2016, 36(6): 587-593 https://doi.org/10.13305/j.cnki.jts.2016.06.005

References

[1] Kachroo A, Robin GP.Systemic signaling during plant defense[J]. Curr Opin Plant Biol, 2013, 16(4): 527-533.
[2] Schweiger R, Heise AM, Persicke M, et al.Interactions between the jasmonic and salicylic acid pathway modulate the plant metabolome and affect herbivores of different feeding types[J]. Plant Cell Environ, 2014, 37(7): 1574-1585.
[3] Dicke M, Baldwin IT.The evolutionary context for herbivore induced plant volatiles: beyond the cry for help[J]. Trends Plant Sci, 2010, 15(3): 167-175.
[4] Wu JQ, Baldwin IT.New insights into plant responses to the attack frominsect herbivores[J]. Annu Rev Genet, 2010, 44: 1-24.
[5] Engelberth J, Contreras CF, Dalvi C, et al.Early transcriptome analyses of Z-3-Hexenol-treated Zea mays revealed distinct transcriptional networks and anti-herbivore defense potential of green leaf volatiles[J]. PLoS ONE, 2013, 8(10): e77465.
[6] Scala A, Allmann S, Mirabella R, et al.Green leaf volatiles: a plant’s multifunctional weapon against herbivores and pathogens[J]. Int J Mol Sci, 2013, 14(9): 17781-17811.
[7] Baldwin IT, Schultz JC.Rapid changes in tree leaf chemistry induced by damage: Evidence for communication between plants[J]. Science, 1983, 221(4607): 277-279.
[8] Kessler A, Baldwin IT.Plant responses to insect herbivory: the emerging molecular analysis[J]. Annual Rev Plant Biol, 2002, 53: 299-328.
[9] Arimura GI, Ozawa R, Nishioka T, et al.Herbivore-induced volatiles induced the emission of ethylene in neighboring lima bean plants[J]. Plant J, 2002, 29(1): 87-98.
[10] Tscharntke T, Thiessen S, Dolch R, et al.Herbivory, induced resistance, and interplant signal transfer in Alnus glutinosa[J]. Biochem Syst Ecol, 2001, 29(10): 1025-1047.
[11] Farmer EE.Surface-to-air signal[J]. Nature, 2001, 411(6839): 854-856.
[12] 穆丹, 付建玉, 刘守安, 等. 虫害诱导的植物挥发物代谢调控机制研究进展[J]. 生态学报, 2010, 30(15): 4221-4233.
[13] Xin Z, Zhang Z, Chen Z, et al.Salicylhydroxamic acid (SHAM) negatively mediates tea herbivore-induced direct and indirect defense against the tea geometrid Ectropis obliqua[J]. J Plant Res, 2014, 127(4): 565-572.
[14] Xin Z, Li X, Li J, et al.Application of chemical elicitor (Z)-3-hexenol enhances direct and indirect plant defenses against tea geometrid Ectropis obliqua[J]. BioControl, 2016, 61(1): 1-12.
[15] Wang GC, Liang HY, Sun XL, et al. Antennal olfactory responses of Apanteles sp. (Hymenoptera: Braconidae) to herbivore-induced plant volatiles [J]. Adv Mater Res, 2012, 393/394/395: 604-607.
[16] Sun XL, Wang GC, Gao Y, et al.Volatiles emitted from tea plants infested by Ectropis obliqua larvae are attractive to conspecific moths[J]. J Chem Ecol, 2014, 40(10): 1080-1089.
[17] Sun XL, Li XW, Xin ZJ, et al.Development of synthetic volatile attractant for male Ectropis obliqua moths[J]. J Integr Agr, 2016, 15(7): 1532-1539.
[18] Yang ZW, Duan XN, Jin S, et al.Regurgitant derived from the tea geometrid Ectropis obliqua suppresses wound-induced polyphenol oxidases activity in tea plants[J]. J Chem Ecol, 2013, 39(6): 744-751.
[19] 孙晓玲, 蔡晓明, 马春雷, 等. 茉莉酸甲酯和机械损伤对茶树叶片多酚氧化酶时序表达的影响[J]. 西北植物学报, 2011, 31(9): 1805-1810.
[20] Liu S, Han B.Differential expression pattern of an acidic 9/13 lipoxygenase in flower opening and senescence and in leaf response to phloem feeders in the tea plant[J]. BMC Plant Biol, 2010, 10(1): 228-243.
[21] 张亚丽, 乔小燕, 陈亮. 茶树ACC氧化酶基因全长cDNA的克隆与表达分析[J] 茶叶科学, 2008, 28(6): 459-467.
[22] Mithofer A, Wanner G, Boland W.Effects of feeding Spodoptera littoralis on Lima bean leaves. II. Continuous mechanical wounding resembling insect feeding is sufficient to elicit herbivory-related volatile emission[J]. Plant Physiol, 2005, 137(3): 1160-1168.
[23] Hilker M, Meiners T.Early herbivore alert: insect eggs induce plant defense[J]. J Chem Ecol, 2006, 32(7): 1379-1397.
[24] Tamiru A, Bruce TJA, Woodcock CM, et al.Maize landraces recruit egg and larval parasitoids in response to egg deposition by a herbivore[J]. Ecol Lett, 2011, 14: 1075-1083.
[25] Turlings TCJ, Wäckers FL.Recruitment of predators and parasitoids by herbivore-damaged plants [M]//Cardé RT, Millar J. Advances in insect chemical ecology. Cambridge: Cambridge University Press, 2004: 21-75.
[26] Kim J, Felton GW.Priming of antiherbivore defensive responses in plants[J]. Insect Sci, 2013, 20(3): 273-285.
[27] Delory BM, Delaplace P, Fauconnier ML, et al.Root-emitted volatile organic compounds: can they mediate belowground plant-plant interactions?[J]. Plant Soil, 2016, 402(1): 1-26.
[28] Arimura G, Ozawa R, Shimoda T, et al.Herbivory-induced volatiles elicit defence genes in lima bean leaves[J]. Nature, 2000, 406(6795): 512-514.
[29] Arimura G, Kost C, Boland W.Herbivore-induced, indirect plant defences[J]. BBA-Mol Cell Biol L, 2005, 1734(2): 91-111.
[30] Cai XM, Sun XL, Dong WX, et al.Herbivore species, infestation time, and herbivore density affect induced volatiles in tea plants[J]. Chemoecology, 2014, 24(1): 1-14.
[31] Engelberth J, Alborn HT, Schmelz EA, et al.Airborne signals prime plants against insect herbivore attack[J]. Proc Natl Acad Sci USA, 2004, 101(6): 1781-1785.
[32] Kessler A, Halitschke R, Diezel C, et al.Priming of plant defense responses in nature by airborne signaling between Artemisia tridentata and Nicotiana attenuata[J]. Oecologia, 2006, 148(2): 280-292.
[33] Matthes MC, Bruce TJA, Ton J, V, et al. The transcriptome of cis-jasmone-induced resistance in Arabidopsis thaliana and its role in indirect defence[J]. Planta, 2010, 232(5): 1163-1180.