Effects of &#x02018;Eurotium cristatum Loose Tea&#x02019; and &#x02018;Eurotium cristatum Powder&#x02019; on the Expressions of JAK2/STAT3 Inflammation and Phosphorylated Proteins in Lung Tissue of Passive Smoking Mice

ZENG Hongzhe; HUANG Xiangxiang; YU Lijun; ZHOU Yufei; XU Shuai; QU Furong

doi:10.13305/j.cnki.jts.2020.02.003

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Journal of Tea Science ›› 2020, Vol. 40 ›› Issue (2) : 165-172. DOI: 10.13305/j.cnki.jts.2020.02.003

Effects of ‘Eurotium cristatum Loose Tea’ and ‘Eurotium cristatum Powder’ on the Expressions of JAK2/STAT3 Inflammation and Phosphorylated Proteins in Lung Tissue of Passive Smoking Mice

ZENG Hongzhe¹, HUANG Xiangxiang¹, YU Lijun^1,2,3*, ZHOU Yufei¹, XU Shuai¹, QU Furong¹

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Abstract

In order to investigate the prevention and recovery mechanism of ‘Eurotium cristatum Loose Tea’ and its ‘Eurotium cristatum powder’ on mouse lung tissues which were injured by passive smoking, passive cigarette smoking environment (CSE) model on SPF C57BL/6 female mice were established. Mice were fed by 600 mg∙kg^-1 Eurotium cristatum tea extract (ECTE) and Eurotium cristatum powder extract (ECPE). Comparing with the CSE model mice, the morphology integrity of lung tissue in passive smoking mice feeding with ECPE and ECTE were significantly protected by observing the pathological slice of lung tissue. The up-regulation levels of IL-6, IL-8, IL-1β, IFN-γ and TNF-α in the serum of mice were inhibited by ELISA analysis. Western blot results show that the expression levels of p-JAK2, p-STAT3, p-JAK2/JAK2, p-STAT3/STAT3 in lung tissues of passive smoking mice fed with ECPE and ECTE were inhibited. These results reveal the prominent protective roles of ECPE and ECTE in the lung injury of passive smoking mice. As a whole, ECPE feeding groups were superior to ECTE feeding groups, while prevention groups were better than treatment groups.

Key words

Eurotium cristatum loose tea / Eurotium cristatum powder / inflammatory cytokines / JAK2/STAT3 signaling pathway / passive smoking

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ZENG Hongzhe, HUANG Xiangxiang, YU Lijun, ZHOU Yufei, XU Shuai, QU Furong. Effects of ‘Eurotium cristatum Loose Tea’ and ‘Eurotium cristatum Powder’ on the Expressions of JAK2/STAT3 Inflammation and Phosphorylated Proteins in Lung Tissue of Passive Smoking Mice[J]. Journal of Tea Science. 2020, 40(2): 165-172 https://doi.org/10.13305/j.cnki.jts.2020.02.003

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