关键词: 普洱茶, 遗传毒性, 精子畸形试验, 骨髓微核试验, 鼠沙门氏伤寒杆菌回复突变试验

Abstract: The investigation on the safely evaluation based on the genetic toxicity was conducted on those three representative on Puer tea sample with one year, five years and ten years storage period produced by the factory of Yunnan by using the classical testing method and used the roasted green tea as the control. The results of mice bone marrow micronuclei test, the mice abnormal spermatozoon test and the Ames test of four tea samples showed negative reaction, Including no mutagenicity on primitive cells, authentic cells and reproductive cells by tested Pu’er tea and roasted green tea samples. The experiment excludes the mutagenicty including the chromosome breakage damage, gene base-pair substitution, and frame shift induced by Pu’er tea. Investigation also discovered that the test samples showed antagonic on the increase of micronucleus rate induced by other factors. Results showed that there four tea samples showed a higher safety to human cell and the Pu’er teas produced by the regular factory are safe to consumer.

Key words: Pu’er tea, Genetic Toxicity, Abnormal spermatozoon test, Micronucleus test, Ames test