茶炭疽病菌毒素的致病活性及理化性质初探

刘守安; 韩宝瑜; 付建玉; 崔林; 李刚

doi:10.13305/j.cnki.jts.2007.02.010

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茶叶科学 ›› 2007, Vol. 27 ›› Issue (2) : 153-158. DOI: 10.13305/j.cnki.jts.2007.02.010

茶炭疽病菌毒素的致病活性及理化性质初探

刘守安, 韩宝瑜^*, 付建玉, 崔林, 李刚^*

作者信息 +

Preliminary Studies on Pathogenic Activity and Physical and Chemical Characteristics of Toxins from Gloeosporium theae-sinensis

LIU Shou-an, HAN Bao-yu^*, FU Jian-yu, CUI Lin, LI Gang^**

Author information +

文章历史 +

摘要

研究发现茶炭疽病菌Gloeosporium theae-sinensis Miyake产生有致病力的外毒素,引起茶树叶片坏死,形成枯斑,类似于病原菌侵染形成的症状。对该菌的培养毒素滤液进行了生物测定,以探讨最佳产毒条件和理化性质。得最佳产毒条件：pH=5.0、25℃且于Czapek-Dox培养液中连续振荡培养16βd。将茶梢浸入毒素滤液而感毒,而后用于茶炭疽病菌毒素生物测定的方法比较合适。

Abstract

Tea anthracnose fungi, Gloeosporium theae-sinensis, can produce pathogenic exo-toxin, which induced the lesions of tea leaves and the symptoms being similar to those resulted from G. theae-sinensis in the fields. The cultural filtrate of G. theae-sinensis was used to toxin bioassay, so as to make a study of the optimum conditions for toxin-producing and characteristics of toxins. The optimum toxin-producing conditions were as follows: pH 5.0, and 25℃ cultivating for 16 days in the manner of vibration with Czapek-Dox liquid medium. The method of immerging the tea shoots into toxin solution was appropriate for the bioassay of toxins.

导出引用

刘守安, 韩宝瑜, 付建玉, 崔林, 李刚. 茶炭疽病菌毒素的致病活性及理化性质初探[J]. 茶叶科学. 2007, 27(2): 153-158 https://doi.org/10.13305/j.cnki.jts.2007.02.010

LIU Shou-an, HAN Bao-yu, FU Jian-yu, CUI Lin, LI Gang. Preliminary Studies on Pathogenic Activity and Physical and Chemical Characteristics of Toxins from Gloeosporium theae-sinensis[J]. Journal of Tea Science. 2007, 27(2): 153-158 https://doi.org/10.13305/j.cnki.jts.2007.02.010

中图分类号： S571.1 S432

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