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茶叶科学 ›› 2007, Vol. 27 ›› Issue (3): 201-210.doi: 10.13305/j.cnki.jts.2007.03.011

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云南普洱茶特征成分的功能与毒理学评价

龚加顺1, 陈文品2, 周红杰3, *, 董兆君4, 张以芳5   

  1. 1. 云南农业大学食品科技学院, 云南 昆明 650201;
    2. 西南农业大学食品科学学院,重庆 400716;
    3. 云南农业大学茶学系,云南 昆明 650201;
    4. 第三军医大学军事毒理学教研室, 重庆 400038;
    5. 云南农业大学动物科学技术学院,云南 昆明 650201
  • 收稿日期:2007-03-04 修回日期:2007-06-03 出版日期:2007-09-25 发布日期:2019-09-11
  • 作者简介:龚加顺(1971— )男,云南富源人,博士,博士后,副教授,主要从事普洱茶和食品生物技术研究。
  • 基金资助:
    云南省自然科学基金重点项目资助(编号:2003C0007Z)

Evaluation on the Function and Toxicity of Extraction of Characteristic Components in Yunnan pu-erh Tea

GONG Jia-shun1, CHEN Wen-ping2, ZHOU Hong-jie3, *, DONG Zhao-jun4, ZHANG Yi-fang5   

  1. 1. Faculty of Food Science and Technology, Yunnan Agricultural University, Kunming 650201, China;
    2. College of Food Science, Southwest University, Chongqing 400716, China;
    3. Department of Tea Science, Yunnan Agricultural University, Kunming 650201, China;
    4. Department of Military Toxicology , Third Military Medical University, Chongqing 400038, China;
    5. College of Animal Science and Technology, Yunnan Agricultural University, Kunming 650201, China
  • Received:2007-03-04 Revised:2007-06-03 Online:2007-09-25 Published:2019-09-11

摘要: 研究了普洱茶特征成分茶褐素、茶多糖与蛋白质等的复合体对昆明种小白鼠的抗疲劳、降胆固醇以及毒理学效应。实验结果显示:普洱茶特征成分提取物能显著提高小白鼠的抗疲劳作用和降低小鼠血液中的胆固醇含量,且优于普洱茶水提物。普洱茶特征成分提取物经口LD50>10βg/kg,属实际无毒级物质。Ames实验中加S9混合液和不加S9混合液的各剂量组回变菌落数与阴性对照差别无统计学意义,且无剂量反应关系。小鼠骨髓嗜多染红细胞微核试验与阴性对照组比较差别无统计学意义。普洱茶特征成分提取物在500~5β000βμg/ml浓度范围内未见诱发CHL细胞染色体畸变率增高。这说明,在实验条件下,普洱茶特征成分提取物属实际无毒级,也未见有致突变作用。

关键词: 普洱茶, 特征成分, 抗疲劳, 降胆固醇, 急性毒性, 致突变性

Abstract: :The anti-fatigue, reduced cholesterol function, acute toxicity and the mutagenicity of extraction of characteristic components in Yunnan pu-erh tea were studied using mouse as experimental animals. It had significant function of anti-fatigue and reduced cholesterol for mouse, its effects was better than that of water extracts of pu-erh tea. The oral acute toxicity in mouse,Ames test,micronucleus test in bone narrow and chromosome abnormality test of characteristic components extraction in Yunnan pu-erh tea were also carried out.The oral LD50 value of this extraction in mouse was more than 10.0βg/kg.The number of relevant strain colonies had no remarkable increase in the Ames test with and without S9 mixture, and the relationship of dose-response was also not found. The micronucleus rates and chromosome abnormality rates at all range of doses had no significant difference compared with the control group. At the dose of 500~5β000βμg/ml, the characteristic components extraction could not cause the increase of mutagenic rate of CHL (Chinese Hamster Lung Fibroblast, CHL) cells. It suggested that the extraction of characteristic components in Yunnan pu-erh tea has no mutagenicity for mouse.

Key words: pu-erh tea, characteristic components, anti-fatigue, reduced cholesterol, acute toxicity, mutagenicity

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