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茶叶科学 ›› 2010, Vol. 30 ›› Issue (6): 429-434.doi: 10.13305/j.cnki.jts.2010.06.004

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广东陈香茶后发酵过程中主要微生物种群和酶类活性变化的研究

陈栋1, 李晶晶2, 方祥2, 黄国资1, 乔小燕1, 吴华玲1, 赖兆祥1   

  1. 1. 广东省农业科学院茶叶研究所,广东 广州 510640;
    2. 华南农业大学,广东 广州 510640
  • 收稿日期:2010-06-08 修回日期:2010-10-25 发布日期:2019-09-11
  • 作者简介:陈栋(1961— ),男,广东茂名市人,博士,研究员,主要从事茶叶生化研究。E-mail:chendong1113@sohu.com
  • 基金资助:
    国家茶叶产业技术体系专项,广东省农业科学院院长基金项目(编号20090107)

Study on the Change of Main Microbial Population and Enzyme Activity in Post-fermented Process of Guangdong Crusted Tea

CHEN Dong1, LI Jing-jing2, FANG Xiang2, HUANG Guo-zi1, QIAO Xiao-yan1, WU Hua-ling1, LAI Zhao-xiang1   

  1. 1. Tea Research Institute, Guangdong Academy of Agriculture Sciences, Guangzhou 510640, China;
    2. South China Agricultural University, Guangzhou 510640, China
  • Received:2010-06-08 Revised:2010-10-25 Published:2019-09-11

摘要: 以岭头单丛茶树品种晒青毛茶为材料进行后发酵加工陈香茶,重点跟踪研究后发酵过程不同阶段的微生物种群、主要生化成分及主导酶类的变化并进行鉴定和分析。结果表明,在陈香茶后发酵过程中,微生物以产黄青霉和黑曲霉为主。茶多酚、茶黄素、茶红素、游离氨基酸等茶叶品质成分含量均呈下降趋势,而茶褐素逐步增加;果胶酶、纤维素酶等促进茶叶品质形成的酶类在后发酵一翻(4 d)时活力到达最高峰,多酚氧化酶在整个过程中活性较低。

关键词: 广东陈香茶, 岭头单丛, 微生物, 酶活性, 化学成分

Abstract: Treated Lingtou Dancong as materials to make crusted tea. The change of microbial population, biochemical composition and dominant enzymes were researched at the different stages in the post-fermented process. The results showed that the Penicillium chrysogenum and Aspergillus were the main microbial population. The contend of biochemical composition related to tea quality were decreased, such as tea polyphenols, theaflavins, thearubigins and free amino acids , while caffeine and theabrown were increased. The bioactivity of Pectinase and cellulase reached to the peak at the third stage. Polyphenol oxidase activity was low in the whole process.

Key words: Guangdong crusted tea, Lingtou Dancong, microbial, enzymes activity, chemical composition

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