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茶叶科学 ›› 2012, Vol. 32 ›› Issue (5): 426-431.doi: 10.13305/j.cnki.jts.2012.05.012

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高EGCG茶树品系杂交技术研究

涂良剑, 林用松, 黄学敏, 王栋, 郑金贵*   

  1. 福建农林大学农产品品质研究所,福建 福州 350002
  • 收稿日期:2012-04-05 修回日期:2012-07-30 出版日期:2012-10-15 发布日期:2019-09-05
  • 通讯作者: fpqri_advisor@163.com
  • 作者简介:涂良剑(1983— ),男,福建大田人,博士研究生,主要从事茶树遗传育种方面的研究。
  • 基金资助:
    农业部948计划项目“台湾优质农产品技术的引进创新与推广”(2011-Z59)、“台湾优质农产品技术的引进创新与推广”(2012-Z30)

Hybridization Technique for Tea Plant Lines with High EGCG Content

TU Liang-jian, LIN Yong-song, HUANG Xue-min, WANG Dong, ZHENG Jin-gui*   

  1. Agricultural Product Quality Institute,Fujian Agriculture and Forestry University,Fuzhou 350002, China
  • Received:2012-04-05 Revised:2012-07-30 Online:2012-10-15 Published:2019-09-05

摘要: 以高EGCG茶树新品系为杂交亲本,利用“剪花瓣显花丝、剪花丝去雄蕊”的人工杂交授粉法,获得了1619个茶树新种质。研究结果表明,采用的杂交授粉技术,操作简便,座果率较高,平均为28.28%±16.26%,最高达46.34%,能够顺利实现茶树杂交育种目标,克服了杂交座果率低的难题;杂交亲本组合对座果率影响最大,其次是杂交苗炭疽病感染率和成苗率,而对种子饱满率和果实种子数影响相对较小。

关键词: 高EGCG茶树品系, 杂交授粉法, 杂交亲和性, 座果率

Abstract: New tea plant germplasm with high EGCG content was used as hybrid parents. The artificial hybridization pollination technique of “cutting petals and thrum shown, cutting thrum and emasculation” was used, and the 1619 tea plants new germplasm are obtained. Meanwhile, the results showed: the new hybridization pollination technique showed a high seed setting rate (an average of 28.28%±16.26%, up to 46.34%) and easy operation, cross-breeding goals can be successfully achieved, and the problem of low hybrid seedling rate could be overcomed. Hybrid parental combination had the greatest effect on seed setting rate, followed by anthracnose infection rate and seedling rate of hybrid seedlings, while relatively small effect on plump seed rate and fruit seed number.

Key words: high EGCG content tea lines, hybridization pollination technique, hybridization compatibility, seedling rate

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