固相萃取结合高效液相制备茶树没食子酸衍生物

doi:10.13305/j.cnki.jts.2012.06.001

茶叶科学 ›› 2012, Vol. 32 ›› Issue (6): 494-499.doi: 10.13305/j.cnki.jts.2012.06.001

固相萃取结合高效液相制备茶树没食子酸衍生物

卢忠尉¹, 蒋晓岚², 刘亚军¹, 刘莉², 高丽萍^1*, 夏涛^2*

1. 安徽农业大学生命科学学院,安徽合肥 230036;
2. 安徽农业大学教育部茶叶生物化学与生物技术重点实验室,安徽合肥 230036

收稿日期:2012-01-06 修回日期:2012-04-28 出版日期:2012-12-15 发布日期:2019-09-05
作者简介:卢忠尉（1986— ）,女,江苏盐城人,硕士研究生,主要从事茶树酚类物质次生代谢研究。
基金资助:
国家自然科学基金（No.30972401、No.31170647和No.31170282）、安徽省自然科学基金（11040606M73）

Preparation of Gallic Acid Derivatives by Solid-phase Extraction Cartridge Coupled with Preparative High Performance Liquid Chromatography from Tea Plant

LU Zhong-wei¹, JIANG Xiao-lan², LIU Ya-jun¹, LIU Li², GAO Li-ping^1*, XIA Tao^2*

1. School of Biology Science, Anhui Agricultural University, Hefei 230036, China;
2. Key Lab of Tea Biochemistry and Biotechnology, Ministry of Education, Anhui Agricultural University, Hefei 230036, China

Received:2012-01-06 Revised:2012-04-28 Online:2012-12-15 Published:2019-09-05

摘要/Abstract

摘要： 没食子酸衍生物是茶树酚酸中的主要成分,是茶汤中重要的鲜味增强剂,具有抗过敏、抗抑郁和增强认知能力等作用。利用固相萃取（solid-phase extraction,SPE）结合制备高效液相色谱（preparative high performance liquid chromatography,PHPLC）,从茶树鲜叶中分离制备出高纯度的3-O-没食子酰奎尼酸和β-D-没食子酰葡萄糖,并利用LC/TOF-ESI-MS、¹H-NMR和¹³C-NMR对它们的结构进行了鉴定。该方法操作简单、纯化效果好。

关键词: 没食子酸衍生物, 固相萃取, 制备高效液相色谱, β-D-没食子酰葡萄糖, 3-O-没食子酰奎尼酸

Abstract: Gallic acid derivatives are the chief compounds of phenolic acid in tea. They are important umami-enhancing compounds in tea beverage, and possess the effects of inhibiting IgE producton, enhancing cognition-anting depressant. A reversed-phase PHPLC coupled with SPE was developed to purify and to prepare high purity 3-O-galloyquinic acid and β-D-glucogalline from fresh tea leaves. The obtained fractions were successfully identified by LC/TOF-ESI-MS, ¹H-NMR and ¹³C-NMR. This method is simple, effective for the preparation of gallic acid derivatives.

Key words: gallic acid derivatives, SPE, PHPLC, β-D-glucogalline, 3-O-galloyquinic acid

中图分类号:

TS272
Q946.8

卢忠尉, 蒋晓岚, 刘亚军, 刘莉, 高丽萍, 夏涛. 固相萃取结合高效液相制备茶树没食子酸衍生物[J]. 茶叶科学, 2012, 32(6): 494-499. doi: 10.13305/j.cnki.jts.2012.06.001.

LU Zhong-wei, JIANG Xiao-lan, LIU Ya-jun, LIU Li, GAO Li-ping, XIA Tao. Preparation of Gallic Acid Derivatives by Solid-phase Extraction Cartridge Coupled with Preparative High Performance Liquid Chromatography from Tea Plant[J]. Journal of Tea Science, 2012, 32(6): 494-499. doi: 10.13305/j.cnki.jts.2012.06.001.

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固相萃取结合高效液相制备茶树没食子酸衍生物

Preparation of Gallic Acid Derivatives by Solid-phase Extraction Cartridge Coupled with Preparative High Performance Liquid Chromatography from Tea Plant

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