固相萃取结合高效液相制备茶树没食子酸衍生物

卢忠尉; 蒋晓岚; 刘亚军; 刘莉; 高丽萍; 夏涛

doi:10.13305/j.cnki.jts.2012.06.001

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茶叶科学 ›› 2012, Vol. 32 ›› Issue (6) : 494-499. DOI: 10.13305/j.cnki.jts.2012.06.001

固相萃取结合高效液相制备茶树没食子酸衍生物

卢忠尉¹, 蒋晓岚², 刘亚军¹, 刘莉², 高丽萍^1*, 夏涛^2*

作者信息 +

Preparation of Gallic Acid Derivatives by Solid-phase Extraction Cartridge Coupled with Preparative High Performance Liquid Chromatography from Tea Plant

LU Zhong-wei¹, JIANG Xiao-lan², LIU Ya-jun¹, LIU Li², GAO Li-ping^1*, XIA Tao^2*

Author information +

文章历史 +

摘要

没食子酸衍生物是茶树酚酸中的主要成分,是茶汤中重要的鲜味增强剂,具有抗过敏、抗抑郁和增强认知能力等作用。利用固相萃取（solid-phase extraction,SPE）结合制备高效液相色谱（preparative high performance liquid chromatography,PHPLC）,从茶树鲜叶中分离制备出高纯度的3-O-没食子酰奎尼酸和β-D-没食子酰葡萄糖,并利用LC/TOF-ESI-MS、¹H-NMR和¹³C-NMR对它们的结构进行了鉴定。该方法操作简单、纯化效果好。

Abstract

Gallic acid derivatives are the chief compounds of phenolic acid in tea. They are important umami-enhancing compounds in tea beverage, and possess the effects of inhibiting IgE producton, enhancing cognition-anting depressant. A reversed-phase PHPLC coupled with SPE was developed to purify and to prepare high purity 3-O-galloyquinic acid and β-D-glucogalline from fresh tea leaves. The obtained fractions were successfully identified by LC/TOF-ESI-MS, ¹H-NMR and ¹³C-NMR. This method is simple, effective for the preparation of gallic acid derivatives.

导出引用

卢忠尉, 蒋晓岚, 刘亚军, 刘莉, 高丽萍, 夏涛. 固相萃取结合高效液相制备茶树没食子酸衍生物[J]. 茶叶科学. 2012, 32(6): 494-499 https://doi.org/10.13305/j.cnki.jts.2012.06.001

LU Zhong-wei, JIANG Xiao-lan, LIU Ya-jun, LIU Li, GAO Li-ping, XIA Tao. Preparation of Gallic Acid Derivatives by Solid-phase Extraction Cartridge Coupled with Preparative High Performance Liquid Chromatography from Tea Plant[J]. Journal of Tea Science. 2012, 32(6): 494-499 https://doi.org/10.13305/j.cnki.jts.2012.06.001

中图分类号： TS272 Q946.8

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