关键词: 粉虱拟青霉, 形态, 培养特征, 生物学特性, 大量培养, 寄主范围, 黑刺粉虱

Abstract: The morphology and the cultural characteristics on several culture media of Paecilomyces aleurocanthus were described in detail. The growth of hyphae, sporulation, conditions of mass production and host range of this fungus were studied. The results showed that the growth of hyphae on PDA and Bran-glucose Agar media was faster than that on Czapek, SDA and SMAY media. The diameters of the colony on the two types of medium were 40-42 mm, 29-35 mm respectively after 19 days’ cultivation. The sporulation of this fugus was slow, the yield of the sporulation was evidently promoted when adding nitrogen source in medium or increasing illumination time. The medium (10% wheat bran and 2% sucrose) was most suitable for liquid fermentation among 5 media; the yield of the sporulation in solid fermentation using rice shell added wheat bran was 1.6-6×108 spores per gram, yet the yield of the sporulation using static culture in shallow pan could get to 6.4-30×108 spores per gram. Artificial inoculation and field investigation showed that the Paecilomyces aleurocanthus fungi could parasitize those insect pests including Aleurocanthus spiniferus, Aspidiotus destructor and Trialeurodes vaporariorum, but couldn’t parasitize those insect pests including Ectropis obliqua 、Toxoptera aurantii 、Acaphylla theae and Empoasca vitis.

Key words: Paecilomyces aleurocanthus, Morphology, Cultural characteristics, Biological character, Mass production, Host range, Aleurocanthus spiniferus