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茶叶科学 ›› 2023, Vol. 43 ›› Issue (6): 784-794.

• 研究报告 • 上一篇    下一篇

EGCG对非肥胖型糖尿病大鼠肾损伤的改善作用及调节机制研究

彭丽媛, 曾鸿哲, 万丽玮, 文帅, 刘昌伟, 安勤, 鲍肃都, 黄建安*, 刘仲华*   

  1. 湖南农业大学茶学教育部重点实验室,国家植物功能成分利用工程技术研究中心,植物功能成分利用省部共建协同创新中心,农业农村部园艺作物基因资源评价利用重点实验室,湖南 长沙 410128
  • 收稿日期:2023-09-11 修回日期:2023-11-05 出版日期:2023-12-15 发布日期:2024-01-08
  • 通讯作者: *Jian7513@hunau.edu.cn;larkin-liu@163.com
  • 作者简介:彭丽媛,女,硕士研究生,研究方向为茶叶功能成分利用与深加工。
  • 基金资助:
    国家茶叶产业技术体系(CARS19)、茶叶功能成分优异资源高效利用技术研究(湘财农指[2021]0015号)

The Investigation of the Ameliorate Effect and Mechanism of EGCG on Non-obese GK Rat with Diabetic Kidney Damage

PENG Liyuan, ZENG Hongzhe, WAN Liwei, WEN Shuai, LIU Changwei, AN Qin, BAO Sudu, HUANG Jian'an*, LIU Zhonghua*   

  1. Key Lab of Education Ministry of Hunan Agricultural University for Tea Science, National Research Center of Engineering and Technology for Utilization of Botanical Functional Ingredients, Co-Innovation Center of Education Ministry for Utilization of Botanical Functional Ingredients, Key Laboratory for Evaluation and Utilization of Gene Resources of Horticultural Crops, Ministry of Agriculture and Rural Affairs of China, Changsha 410128, China
  • Received:2023-09-11 Revised:2023-11-05 Online:2023-12-15 Published:2024-01-08

摘要: EGCG是一种抗氧化、抗炎症的天然活性成分,目前关于EGCG的抗氧化和抗炎症作用在糖尿病肾损伤中的作用及调节机制研究较少。采用不同剂量的EGCG干预非肥胖型糖尿病Goto-Kakizaki(GK)大鼠以探究EGCG对糖尿病大鼠肾损伤的作用和机制。试验周期为4周,监测试验期内大鼠的平均体质量和日平均摄食量,并在试验结束后取血清和肾脏组织,检测大鼠肾功能、肾脏病理指标、氧化应激和炎症指标以及Nrf2-Keap1/MAPK信号通路相关基因表达水平。试验表明,EGCG能够有效改善GK大鼠肾脏形态损伤,显著降低血肌酐、尿素氮和肾脏丙二醛含量,提高肾脏超氧化物歧化酶、过氧化氢酶和谷胱甘肽过氧化物酶等抗氧化酶活性,抑制促炎因子单核细胞趋化蛋白-1和白介素-1β的释放水平,调节与抗氧化应激相关的Nrf2-Keap1/MAPK信号通路的Nrf2Keap1JNKNF-κBP38基因表达水平,且在试验范围内,高剂量的改善效果优于低剂量。以上结果表明,EGCG对大鼠糖尿病导致的肾损伤有一定的改善作用,其作用机理可能与Nrf2-Keap1/MAPK信号通路介导的抗氧化应激有关。

关键词: EGCG, 糖尿病肾损伤, 氧化应激, 炎症, 糖尿病, GK大鼠

Abstract: Epigallocatechin gallate (EGCG) is an antioxidant and anti-inflammatory natural active ingredient, and fewer studies have been conducted on the antioxidant and anti-inflammatory effects of EGCG in DKD and the regulatory mechanisms. This study investigated the effect and mechanism of EGCG on diabetic kidney damage in non-obese GK rats with idiopathic T2DM. Two different doses of EGCG (10 mg·kg-1 and 120 mg·kg-1) were administered to GK rats for 4 weeks. The body weight and daily food intake of rats were monitored during the experiment. At the end of the experiment, the serum and kidney tissues were collected to detect some kidney biochemical and pathological indicators and Nrf2-Keap1/MAPK signaling pathway related gene expression levels. The results show that EGCG could improve the kidney morphology and significantly increase the activities of antioxidant enzymes (such as SOD, CAT and GSH-Px), and inhibit the release of proinflammatory cytokines (MCP-1, IL-1β). In addition, EGCG could restrain oxidant stress by up-regulate the expression level of Nrf2 and inhibit inflammation by down-regulating the expression levels of JNK, NF-κB and P38 genes in kidney. The improvement effect of high dose was better than that of low dose in the experimental range. In conclusion, these results indicate that EGCG could ameliorate kidney injury caused by diabetes, and its mechanism might be related to anti-oxidative stress mediated by Nrf2-Keap1/MAPK signaling pathways.

Key words: EGCG, diabetic kidney damage, oxidant stress, inflammation, diabetes, GK rats

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