酶法拆分DL-茶氨酸及其分离纯化

doi:10.13305/j.cnki.jts.2006.01.005

茶叶科学 ›› 2006, Vol. 26 ›› Issue (1): 31-36.doi: 10.13305/j.cnki.jts.2006.01.005

酶法拆分DL-茶氨酸及其分离纯化

郭丽芸,刘毅,贾晓娟,李兆兰,焦庆才

南京大学医药生物技术国家重点实验室, 江苏南京, 210093

收稿日期:2005-07-01 修回日期:2005-10-10 出版日期:2006-03-25 发布日期:2019-09-10
作者简介:郭丽芸（1981— ）,女,硕士研究生,从事手性药物的合成及拆分。E-mail：guoliyun03@163.com
基金资助:
国家技术创新基金（02CJ-13-01-16）

Resolution of DL-theanine by Aminoacylase and the Separation of Productions

GUO Li-yun, LIU Yi, JIA Xiao-juan, LI Zhao-lan, JIAO Qing-cai

State Key Laboratory of Pharmaceutical Biotechnology, Nanjing University, Nanjing 210093, China

Received:2005-07-01 Revised:2005-10-10 Online:2006-03-25 Published:2019-09-10

摘要/Abstract

摘要： 将DL-茶氨酸乙酰化为N-乙酰-DL-茶氨酸,利用本实验室筛选的有较高氨基酰化酶活性的真菌刺孢小克银汉霉9980（Cunnighamella echinulata 9980）对DL-茶氨酸进行拆分并对拆分条件进行摸索。结果表明：反应最适温度50℃,最适pH7.0,底物浓度0.5mol/L,湿菌体量4g/100βml,拆分时间30h,拆分率可达92％。产物经JK008阳离子交换树脂氨性柱分离,L-茶氨酸收率84.3％,[α] =8.1(c=2,H₂O), 符合JP2000药典。

关键词: L-茶氨酸, N-乙酰-DL-茶氨酸, 刺孢小克银汉霉9980, 氨基酰化酶, 光学拆分

Abstract: An enzymatic method for the optical resolution of DL-theanine by aminoacylases from Cunnighamella echinulata 9980 was developed. The optimum temperature, pH and substrate concentration were: 50℃, pH7.0, and 0.5mol/L N-Ac-DL-theanine. The conversion rate against N-Ac-L-theanine was 92%. Productions were purified on JK008 ion exchange resin. The yield of L-theanine was 84.3%, [α] =8.1(c=2,H₂O), complied with JP2000。

Key words: L-theanine, N-Ac-DL- theanine, Cunnighamella echinulata 9980, aminoacylase, optical resolution

中图分类号:

郭丽芸, 刘毅, 贾晓娟, 李兆兰, 焦庆才. 酶法拆分DL-茶氨酸及其分离纯化[J]. 茶叶科学, 2006, 26(1): 31-36. doi: 10.13305/j.cnki.jts.2006.01.005.

GUO Li-yun, LIU Yi, JIA Xiao-juan, LI Zhao-lan, JIAO Qing-cai. Resolution of DL-theanine by Aminoacylase and the Separation of Productions[J]. Journal of Tea Science, 2006, 26(1): 31-36. doi: 10.13305/j.cnki.jts.2006.01.005.

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酶法拆分DL-茶氨酸及其分离纯化

Resolution of DL-theanine by Aminoacylase and the Separation of Productions

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