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茶叶科学 ›› 2007, Vol. 27 ›› Issue (2): 153-158.doi: 10.13305/j.cnki.jts.2007.02.010

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茶炭疽病菌毒素的致病活性及理化性质初探

刘守安, 韩宝瑜*, 付建玉, 崔林, 李刚*   

  1. 农业部茶叶化学工程重点开放实验室,中国农业科学院茶叶研究所, 浙江 杭州 310008
  • 收稿日期:2006-10-26 修回日期:2007-03-08 出版日期:2007-06-25 发布日期:2019-09-23
  • 通讯作者: *韩宝瑜,han-insect@263.net,**温州医学院生命科学院实习生。
  • 作者简介:刘守安(1980— ),男,山东泰安人,硕士,主要从事分子植物病理学与微生物的研究。
  • 基金资助:
    中国农业科学院首批二级岗位杰出人才科研基金课题(2002-382)和浙江省151人才工程基金资助课题

Preliminary Studies on Pathogenic Activity and Physical and Chemical Characteristics of Toxins from Gloeosporium theae-sinensis

LIU Shou-an, HAN Bao-yu*, FU Jian-yu, CUI Lin, LI Gang**   

  1. Key Laboratory of Tea Chemical Engineering, Ministry of Agriculture; Tea Research Institute , Chinese Academy of Agricultural Sciences, Hangzhou 310008, China
  • Received:2006-10-26 Revised:2007-03-08 Online:2007-06-25 Published:2019-09-23

摘要: 研究发现茶炭疽病菌Gloeosporium theae-sinensis Miyake产生有致病力的外毒素,引起茶树叶片坏死,形成枯斑,类似于病原菌侵染形成的症状。对该菌的培养毒素滤液进行了生物测定,以探讨最佳产毒条件和理化性质。得最佳产毒条件:pH=5.0、25℃且于Czapek-Dox培养液中连续振荡培养16βd。将茶梢浸入毒素滤液而感毒,而后用于茶炭疽病菌毒素生物测定的方法比较合适。

关键词: 茶炭疽病菌, 生物测定, 培养条件, 毒素

Abstract: Tea anthracnose fungi, Gloeosporium theae-sinensis, can produce pathogenic exo-toxin, which induced the lesions of tea leaves and the symptoms being similar to those resulted from G. theae-sinensis in the fields. The cultural filtrate of G. theae-sinensis was used to toxin bioassay, so as to make a study of the optimum conditions for toxin-producing and characteristics of toxins. The optimum toxin-producing conditions were as follows: pH 5.0, and 25℃ cultivating for 16 days in the manner of vibration with Czapek-Dox liquid medium. The method of immerging the tea shoots into toxin solution was appropriate for the bioassay of toxins.

Key words: gloeosporium theae-sinensis, bioassay, cultural conditions, toxin

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