普洱茶素Ⅴ~Ⅶ对4种乳腺癌细胞作用研究

doi:10.13305/j.cnki.jts.2024.03.004

茶叶科学 ›› 2024, Vol. 44 ›› Issue (3): 501-514.doi: 10.13305/j.cnki.jts.2024.03.004

普洱茶素Ⅴ~Ⅶ对4种乳腺癌细胞作用研究

杨锐^1,2,6, 赵兴平^1,2,6, 何明婕^1,2,6, 刘敏^1,2,6, 罗蓉^1,2,6, 陈川龙^5,6, 潘淑康^1,2,6,*, 丁章贵^1,2,3,4,6,*

1.云南大益微生物技术有限公司,云南昆明 650217;
2.云南省普洱茶发酵工程研究中心,云南昆明 650217;
3.云南大学生命科学学院云南省微生物研究所,云南昆明 650091;
4.云南大学西南微生物多样性教育部重点实验室,云南昆明 650091;
5.大益集团勐海茶业有限责任公司,云南勐海 666200;
6.农业农村部普洱茶加工技术重点实验室,云南昆明 650217

收稿日期:2024-01-03 修回日期:2024-03-22 出版日期:2024-06-15 发布日期:2024-07-08
通讯作者: *psk.happy@163.com;dzg@ynu.edu.cn
作者简介:杨锐,女,工程师,主要从事茶叶化学成分生物活性方面的研究。
基金资助:
云南省重大科技专项（202002AE32001）、兴滇英才支持计划（YNWR-QNBJ-2019-003）

Study on the Effects of Puerins Ⅴ-Ⅶ on Four Kinds of Breast Cancer Cells

YANG Rui^1,2,6, ZHAO Xingping^1,2,6, HE Mingjie^1,2,6, LIU Min^1,2,6, LUO Rong^1,2,6, CHEN Chuanlong^5,6, PAN Shukang^1,2,6,*, DING Zhanggui^1,2,3,4,6,*

1. Yunnan TAETEA Microbial Technology Co., Ltd., Kunming 650217, China;
2. Fermentation Engineering Research Center for Yunnan Pu-erh Tea, Kunming 650217, China;
3. Yunnan Institute of Microbiology, School of Life Sciences, Yunnan University, Kunming 650091, China;
4. Key Laboratory for Southwest Microbial Diversity of the Ministry of Education, Yunnan University, Kunming 650091, China;
5. Menghai Tea Industry Co., Ltd., TAETEA Group, Menghai 666200, China;
6. Key Laboratory of Pu-erh Tea Processing Technology, Ministry of Agriculture and Rural Affairs, Kunming 650217, China

Received:2024-01-03 Revised:2024-03-22 Online:2024-06-15 Published:2024-07-08

摘要/Abstract

摘要： 普洱茶素（Puerins）是从普洱熟茶中分离得到的儿茶素衍生物,是一类结构独特的含氮多酚化合物。开展普洱茶素Ⅴ~Ⅶ抗乳腺癌细胞作用的研究,以4种不同受体表型的乳腺癌细胞株为模型,进行噻唑蓝（MTT）比色试验、蛋白质组学检测及细胞凋亡检测等试验。结果表明,普洱茶素Ⅴ、Ⅵ和Ⅶ等3个单体化合物对4种乳腺癌细胞都有显著的抑制作用;蛋白质组学分析发现,普洱茶素干预后乳腺癌细胞表达产生差异的蛋白主要集中于代谢通路,其次是癌症通路和黏附斑信号通路;细胞凋亡检测发现3个普洱茶素化合物均可诱导MDA-MB-231细胞的凋亡。本研究报道了普洱茶素Ⅴ~Ⅶ的抗乳腺癌细胞活性及初步作用机制,为研究普洱熟茶抗癌活性物质提供基础信息。

关键词: 普洱熟茶, 普洱茶素, 乳腺癌, 蛋白质组学, 细胞凋亡

Abstract: Puerins, a kind of nitrogen-containing polyphenol compound with unique structure, is a kind of catechin derivative first isolated from ripened Pu-erh tea. In this paper, the effects of puerins Ⅴ-Ⅶ on breast cancer cells were studied. Four breast cancer cell lines with different receptor phenotypes were used as models, and MTT assay, proteomics detection and apoptosis detection were carried out. The results show that all puerins Ⅴ, Ⅵ and Ⅶ had obvious inhibitory effects on the four kinds of breast cancer cells. Proteomics analysis indicates that the differentially expressed proteins were mainly concentrated in the metabolic pathway, followed by cancer pathway and adhesion spot signal pathway under the intervention of puerins Ⅴ-Ⅶ. Apoptosis detection demonstrates that all three puerins compounds could induce the apoptosis of MDA-MB-231 cells. This study first reported the anti-breast cancer activity and preliminary mechanism of puerins Ⅴ-Ⅶ, which provided basic information for studying the anti-cancer active substances of ripened Pu-erh tea.

Key words: ripened Pu-erh tea, puerins, breast cancer, proteomics, apoptosis

中图分类号:

杨锐, 赵兴平, 何明婕, 刘敏, 罗蓉, 陈川龙, 潘淑康, 丁章贵. 普洱茶素Ⅴ~Ⅶ对4种乳腺癌细胞作用研究[J]. 茶叶科学, 2024, 44(3): 501-514. doi: 10.13305/j.cnki.jts.2024.03.004.

YANG Rui, ZHAO Xingping, HE Mingjie, LIU Min, LUO Rong, CHEN Chuanlong, PAN Shukang, DING Zhanggui. Study on the Effects of Puerins Ⅴ-Ⅶ on Four Kinds of Breast Cancer Cells[J]. Journal of Tea Science, 2024, 44(3): 501-514. doi: 10.13305/j.cnki.jts.2024.03.004.

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普洱茶素Ⅴ~Ⅶ对4种乳腺癌细胞作用研究

Study on the Effects of Puerins Ⅴ-Ⅶ on Four Kinds of Breast Cancer Cells

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