EGCG与锌离子互作对PC-3细胞能量代谢的影响

doi:10.13305/j.cnki.jts.2009.4.001

茶叶科学 ›› 2009, Vol. 29 ›› Issue (4): 251-256.doi: 10.13305/j.cnki.jts.2009.4.001

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EGCG与锌离子互作对PC-3细胞能量代谢的影响

孙世利¹, 凌彩金¹, 于海宁², 沈生荣³, 苗爱清¹, 潘顺顺¹, 庞式¹, 卓敏¹, 赵超艺^1,*

1. 广东省农业科学院茶叶研究所,广东广州 510640;
2. 浙江工业大学药学院,浙江杭州 310032;
3. 浙江大学生物系统工程与食品科学学院,浙江杭州 310029

修回日期:2008-10-20 接受日期:2009-01-07 发布日期:2019-09-09
通讯作者: * zhaochaoyi@sogou.com
作者简介:孙世利（1979— ）,男,山东烟台人,博士,从事茶叶功能成分生物活性研究。

Effect of Interactions Between EGCG and Zn²⁺on Energy Metabolism of PC-3 Cells

SUN Shi-li¹, LING Cai-jin¹, YU Hai-ning², SHEN Sheng-rong³, MIAO Ai-qing¹, PAN Shun-shun¹, PANG Shi¹, ZHUO Min¹, ZHAO Chao-yi^1,*

1. Tea Research Institute Guangdong Academy of Agricultural Sciences, Guangzhou 510640, China;
2. College of Pharmaceutical Sciences, Zhejiang University of Technology, Hangzhou 310032, China;
3. Department of Food Science and Nutrition, Zhejiang University, Hangzhou 310029, China

Revised:2008-10-20 Accepted:2009-01-07 Published:2019-09-09

摘要/Abstract

摘要： 采用Hoechst 33258荧光染色法观察EGCG、Zn²⁺和EGCG+Zn²⁺对前列腺癌PC-3细胞凋亡的诱导作用;采用HPLC法检测了EGCG、Zn²⁺和EGCG+Zn²⁺对PC-3细胞内腺苷酸（ATP、ADP、AMP）含量的影响,并分析了能量负荷（EC）和ATP/ADP比值。结果表明,Hoechst 33258染色后正常细胞发出均匀微弱的蓝色荧光,细胞核未见异常,EGCG、Zn²⁺和EGCG+Zn²⁺处理后凋亡的PC-3细胞都发出较强的蓝色荧光,细胞核DNA断裂及染色体高度浓缩;EGCG、Zn²⁺及二者混合物处理后细胞内总腺苷酸含量、EC及ATP/ADP比值都下降,表明EGCG与Zn²⁺对PC-3细胞能量代谢都存在明显的抑制作用。

关键词: EGCG, 锌离子, PC-3细胞, 细胞凋亡, 腺苷酸, 能量代谢

Abstract: In the present work, the effect of treatments with EGCG, Zn²⁺ and EGCG+Zn²⁺ on inducing apoptosis and content of adenylate nucleotide (ATP, ADP and AMP) in PC-3 cells was investigated by chromatin staining with Hoechst 33258 and HPLC. Hoechst 33258 staining revealed the appearance of condensed chromatin and fragmented apoptotic nuclei under fluorescence microscopy at a concentration of 80 µmol/L EGCG, 80 µmol/L Zn²⁺ and 80 µmol/L EGCG+80 µmol/L Zn²⁺ in PC-3 cells. In the determination of adenylate nucleotide, after treated with EGCG, Zn²⁺ and EGCG+Zn²⁺, content of total adenylate nucleotide, EC and ATP/ADP value were significantly decreased in PC-3 cells, which demonstrated that EGCG and Zn²⁺ have a markedly inhibitory effect on energy metabolism of PC-3 cells.

Key words: EGCG, Zn²⁺, PC-3 cells, apoptosis, adenosine triphosphate, energy metabolism

中图分类号:

孙世利, 凌彩金, 于海宁, 沈生荣, 苗爱清, 潘顺顺, 庞式, 卓敏, 赵超艺. EGCG与锌离子互作对PC-3细胞能量代谢的影响[J]. 茶叶科学, 2009, 29(4): 251-256. doi: 10.13305/j.cnki.jts.2009.4.001.

SUN Shi-li, LING Cai-jin, YU Hai-ning, SHEN Sheng-rong, MIAO Ai-qing, PAN Shun-shun, PANG Shi, ZHUO Min, ZHAO Chao-yi. Effect of Interactions Between EGCG and Zn²⁺on Energy Metabolism of PC-3 Cells[J]. Journal of Tea Science, 2009, 29(4): 251-256. doi: 10.13305/j.cnki.jts.2009.4.001.

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EGCG与锌离子互作对PC-3细胞能量代谢的影响

Effect of Interactions Between EGCG and Zn²⁺on Energy Metabolism of PC-3 Cells

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EGCG与锌离子互作对PC-3细胞能量代谢的影响

Effect of Interactions Between EGCG and Zn2+ on Energy Metabolism of PC-3 Cells

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Effect of Interactions Between EGCG and Zn²⁺on Energy Metabolism of PC-3 Cells