茶氨酸对小鼠戊巴比妥钠镇静催眠的双向作用

doi:10.13305/j.cnki.jts.2009.4.002

茶叶科学 ›› 2009, Vol. 29 ›› Issue (4): 257-262.doi: 10.13305/j.cnki.jts.2009.4.002

茶氨酸对小鼠戊巴比妥钠镇静催眠的双向作用

虞希冲¹, 吴波拉¹, 朱桐君¹, 杨伟^2,*

1. 温州医学院药学院药理学教研室,浙江温州 325000;
2. 温州医学院药学院药剂学教研室,浙江温州 325000

修回日期:2008-10-20 接受日期:2009-01-07 发布日期:2019-09-09
通讯作者: *
作者简介:虞希冲（1975— ）,男,硕士,浙江温州人,从事神经药理学和毒理学研究。

Theanine Effects on Sedative and Hypnosis Induced by Pentobarbital Sodium In ICR Mice

YU Xi-chong¹, WU Bo-la¹, ZHU Tong-jun¹, YANG Wei^2,*

1. Department of pharmacology, Wenzhou medical college, Wenzhou 325035, China;
2. Department of pharmacy, Wenzhou medical college, Wenzhou 325035, China

Revised:2008-10-20 Accepted:2009-01-07 Published:2019-09-09

摘要/Abstract

摘要： 采用自发活动仪和观察翻正反射消失与否研究茶氨酸对戊巴巴比妥钠诱导的镇静催眠作用。结果显示：单用茶氨酸能剂量依赖性地抑制正常小鼠的自发活动,其半数抑制量为0.8518 g/kg;较小剂量(≤1.0 g/kg)茶氨酸能加强戊巴比妥钠(5 mg/kg)的抑制自发活动效应,而在2.0 g/kg时则引起自发活动反跳性的增加,呈现”U”型改变;单用茶氨酸5.0 g/kg并无催眠效应,但小于等于5.0 g/kg时茶氨酸加强戊巴比妥钠(25 mg/kg)的催眠作用,并呈明显的剂量依赖性。上述结果表明茶氨酸对戊巴比妥钠诱导的小鼠自发活动影响为双向改变(“U”改变);茶氨酸对戊巴比妥钠(25 mg/kg)的催眠效应呈剂量依赖性地加强作用,并未出现双向改变。

关键词: 茶氨酸, 戊巴比妥钠, 镇静, 催眠

Abstract: In the present study, the locomotor activities and disappearance of righting reflex on ICR mice were employed to evaluate effects of theanine on the sedative and hypnosis induced by pentobarbital sodium. Results showed that theanine alone decreased locomotor activity in dose-dependent manner and had no stimulant effect, 50% inhibition dose of locomotor activity was 0.8518 g/kg, low doses of theanine(≤1.0 g/kg) enhanced inhibition effect of locomotor activity induced by pentobarbital sodium(5 mg/kg), however, high dose (2.0 g/kg) of theanine reversed the inhibition, meanwhile, theanine enhanced hypnosis induced by pentobarbital sodium(25 mg/kg) in dose-dependent manner. It is concluded that theanine changed locomotor activity of mice induced by low dose of pentobarbital sodium(5 mg/kg) in dimensional change (“U” shape style), but not higher dose of pentobarbital sodium(25 mg/kg).

Key words: theanine, pentobarbital, sedation, hypnosis

中图分类号:

TS272
R96

虞希冲, 吴波拉, 朱桐君, 杨伟. 茶氨酸对小鼠戊巴比妥钠镇静催眠的双向作用[J]. 茶叶科学, 2009, 29(4): 257-262. doi: 10.13305/j.cnki.jts.2009.4.002.

YU Xi-chong, WU Bo-la, ZHU Tong-jun, YANG Wei. Theanine Effects on Sedative and Hypnosis Induced by Pentobarbital Sodium In ICR Mice[J]. Journal of Tea Science, 2009, 29(4): 257-262. doi: 10.13305/j.cnki.jts.2009.4.002.

参考文献

[1] 吕毅, 郭雯飞, 倪捷儿, 等. 茶氨酸的生理作用及合成[J]. 茶叶科学, 2003, 23(1): 1~5.
[2] Egashira N, Hayakawa K, Mishima K, et al. Neuroprotective effect of gamma-glutamylethylamide (theanine) on cerebral infarction in mice[J]. Neurosci Lett, 2004, 363(1): 58~61.
[3] Kakuda T, Yanase H, Utsunomiya K, et al. Protective effect of gamma-glutamylethylamide (theanine) on ischemic delayed neuronal death in gerbils[J]. Neurosci Lett, 2000, 289(3): 189~192.
[4] Nagasawa K, Aoki H, Yasuda E, et al. Possible involvement of group I mGluRs in neuroprotective effect of theanine[J]. Biochem Biophys Res Commun, 2004, 320(1): 116~122.
[5] Kakuda T, Nozawa A, Unno T, et al. Inhibiting effects of theanine on caffeine stimulation evaluated by EEG in the rats[J]. Biosci Biotech. Biochem, 2000, 64(2): 287~293.
[6] 王小雪, 邱隽, 宋宇, 等. 茶氨酸的抗疲劳作用研究[J]. 中国公共卫生, 2002, 18(3): 315~317.
[7] Taverna FA, Cameron BR, Wang LY, et al. Sensitivity of AMPA receptors to pentobarbital[J]. Eur J Pharmacol, 1994, 267(3): R3~5.
[8] Yamakura T, Sakimura K, Mishina M, et al. The sensitivity of AMPA-selective glutamate receptor channels to pentobarbital is determined by a single amino acid residue of the alpha 2 subunit[J]. FEBS-Lett, 1995, 374(3): 412~414.
[9] Kakuda T, Nozawa A, Sugimoto A, et al. Inhibition by theanine of binding of [3H]-AMPA, [3H]-kainite, and [3H]-MDL105519 to glutamate receptors[J]. Biosci Biotechnol Biochem, 2002, 66(12): 2683~2686.
[10] Menniti FS, Buchan AM, Chenard BL, et al. CP-465,022, a selective noncompetitive AMPA receptor antagonist, blocks AMPA receptors but is not neuroprotective in vivo[J]. Stroke, 2003, 34: 171~176.
[11] Imre G, Fokkema DS, Den Boer JA, et al. Dose-response characteristics of ketamine effect on locomotion, cognitive function and central neuronal Activity[J]. Brain Research Bull, 2006, 69: 338~345.
[12] Jentsch JD, Roth RH.The neuropsychopharmacology of phencyclidine: from NMDA receptor hypofunction to the dopamine hypothesis of schizophrenia[J]. Neuropsychopharmacology, 1999, 20(3): 201~225.
[13] yokogoshi H, kobayashi M, et al. Effect of theanine, r-glutamylethylamide, on brain monoamines and striatal dopamine release in conscious rats[J]. Neurochem Res, 1998, 23(5): 667~673.

[1]	黄秋萍, 谢晨阳, 李新宇, 金斌, 曾榛, 钱波, 戴玲, 宋家乐. L-茶氨酸改善来曲唑诱导的多囊卵巢综合征大鼠临床症状[J]. 茶叶科学, 2021, 41(6): 831-842.
[2]	陈美艳, 刘芬, 林勇, 左高隆, 左颖鹏, 刘仲华. L-茶氨酸对CUMS抑郁大鼠海马和肠道损伤的干预作用研究[J]. 茶叶科学, 2021, 41(4): 511-524.
[3]	毛鹏, 王丽鸳, 白培贤, 韦康, 阮丽, 张亚真, 成浩. 类茶氨酸合成酶基因家族在番茄中的鉴定及表达研究[J]. 茶叶科学, 2021, 41(2): 173-183.
[4]	金可, 黄建安, 熊立瑰, 刘硕谦, 覃小洪, 彭靖, 李银花, 李娟. 黄化变异茶树石门黄叶理化分析及茶氨酸相关基因表达研究[J]. 茶叶科学, 2021, 41(1): 40-47.
[5]	曾立, 肖文军. L-茶氨酸防治脑疾病研究进展[J]. 茶叶科学, 2019, 39(2): 193-202.
[6]	彭影琦, 肖文军, 张盛, 袁冬寅, 林玲, 周阳, 龚志华. L-茶氨酸对小鼠肠道形态结构及游离氨基酸的影响[J]. 茶叶科学, 2019, 39(1): 43-54.
[7]	朱城波, 吴小妹, 冯丽霞, 黄象鹏, 庞林江, 杨虎清, 方正伟, 王颖, 赖姝毓, 张宜明. 直读显色快速检测绿茶中的茶氨酸[J]. 茶叶科学, 2018, 38(3): 244-252.
[8]	李小杰, 马建强, 姚明哲, 陈亮. 茶氨酸合成酶基因的SNP挖掘和遗传定位[J]. 茶叶科学, 2017, 37(3): 251-257.
[9]	郭晨, 刘林峰, 向奕, 罗莉, 林玲, 肖文军. L-茶氨酸改善热应激引起的小鼠组织损伤和氧化逆境[J]. 茶叶科学, 2017, 37(1): 17-24.
[10]	刘遵莹, 刘秋玲, 邓燕莉, 陈凌, 肖文军. L-茶氨酸对产肠毒素大肠杆菌引起的免疫应激小鼠肠道的保护作用研究[J]. 茶叶科学, 2016, 36(5): 469-476.
[11]	刘秋玲, 龚志华, 陈凌, 刘遵莹, 邓燕莉, 陈栋, 肖文军. L-茶氨酸对产肠毒性大肠杆菌引起的免疫应激小鼠肝脏的保护作用研究[J]. 茶叶科学, 2016, 36(5): 484-490.
[12]	彭彬, 刘仲华, 林勇, 林海燕, 黄建安. L-茶氨酸改善慢性应激大鼠抑郁行为作用研究[J]. 茶叶科学, 2014, 34(4): 355-363.
[13]	刘晓慧, 揭国良, 林康, 吴媛媛, 王岳飞, 屠幼英. EGCG和茶氨酸对细胞氧化损伤的协同保护和修复作用研究[J]. 茶叶科学, 2014, 34(3): 239-247.
[14]	虞希冲, 杨伟, 吴波拉. 茶氨酸经下丘脑腹外侧核抑制雌性小鼠摄食与胃排空作用研究[J]. 茶叶科学, 2013, 33(5): 429-435.
[15]	谭俊峰, 林智, 彭群华, 郭丽, 吕海鹏. 茶氨酸复合制剂缓解体力疲劳功能评价[J]. 茶叶科学, 2012, 32(6): 530-534.

茶氨酸对小鼠戊巴比妥钠镇静催眠的双向作用

Theanine Effects on Sedative and Hypnosis Induced by Pentobarbital Sodium In ICR Mice

PDF (PC)

可视化

被引次数

摘要/Abstract

引用本文

使用本文

参考文献

相关文章 15

Metrics

本文评价

推荐阅读 10