茶氨酸经下丘脑腹外侧核抑制雌性小鼠摄食与胃排空作用研究

虞希冲; 杨伟; 吴波拉

doi:10.13305/j.cnki.jts.2013.05.008

PDF(322 KB)

茶叶科学 ›› 2013, Vol. 33 ›› Issue (5) : 429-435. DOI: 10.13305/j.cnki.jts.2013.05.008

茶氨酸经下丘脑腹外侧核抑制雌性小鼠摄食与胃排空作用研究

虞希冲^1,2, 杨伟³, 吴波拉²

作者信息 +

Theanine Depressed the Food Intake and Gastric Emptying in Female Mice via Lateral Hypothalamic AMPA and NMDA Receptor

YU Xi-chong^1,2, YANG Wei³, WU Bo-la²

Author information +

文章历史 +

摘要

采用比色法观察脑室、核团内微注射和腹腔注射茶氨酸对外周胃排空的影响。结果表明,腹腔给予茶氨酸3~30βmg/kg后显著抑制摄食量和胃排空;脑室给药3~100βng后,对胃排空的影响表现出V型曲线,3~30βng茶氨酸剂量依赖性抑制胃排空,50、100βng茶氨酸使胃排空恢复到正常水平。然而,腹腔注射同样量的茶氨酸并无抑制作用。在下丘脑外侧核内注射同量茶氨酸,出现与脑室内类似的抑制胃排空作用,在弓状核、下丘脑腹内侧核内注射却无明显的改变。在下丘脑外侧核内注射NMDA和AMPA后均能诱导摄食和胃排空的增加,而茶氨酸10、30、100βng能抑制两者诱导的胃排空及NMDA诱导的摄食,茶氨酸3~100βng能抑制AMPA诱导的摄食。上述结果表明茶氨酸抑制摄食和胃排空作用可能与抑制下丘脑外侧核的NMDA受体和AMPA受体有关。

Abstract

In the present study, the food intake and gastric emptying of female mice were evaluated after theanine microinjection in cerebral ventrile, lateral hypothalamus, arcurate nuleius and ventromedial hypothalamic nucleus. Results showed that theanine 3~30βmg/kg intraperitoneal injection decreased food intake and gastric emptying; theanine 3~100βng microinjection into cerebral ventrile induced “V” style effects on gastric, theanine 3~30βng decreased gastric emptying dose-dependently while theanine 50 and 100βng recovered gastric emptying. Theanine microinjection in lateral hypothalamus displayed similar effects on gastic emptying as theanine i.c.v while microinjection in arcurate nucleus and ventromedial hypothalamic nucleus did not alert gastric emptying. Theanine decreased food intake and gastric emptying induced by NMDA and AMPA microinjection in lateral hypothalamus. It

导出引用

虞希冲, 杨伟, 吴波拉. 茶氨酸经下丘脑腹外侧核抑制雌性小鼠摄食与胃排空作用研究[J]. 茶叶科学. 2013, 33(5): 429-435 https://doi.org/10.13305/j.cnki.jts.2013.05.008

YU Xi-chong, YANG Wei, WU Bo-la. Theanine Depressed the Food Intake and Gastric Emptying in Female Mice via Lateral Hypothalamic AMPA and NMDA Receptor[J]. Journal of Tea Science. 2013, 33(5): 429-435 https://doi.org/10.13305/j.cnki.jts.2013.05.008

中图分类号： Q517

参考文献

[1] Yokogoshi H, Kobayashi M.Hypotensive effect of gamma-glutamylmethylamide in spontaneously hypertensive rats[J]. Life Sci, 1998, 62(12): 1065-1068.
[2] Sugiyama T, Sadzuka Y.Theanine and glutamate transporter inhibitors enhance the antitumor efficacy of chemotherapeutic agents[J]. Biochim Biophys Acta, 2003, 1653(2): 47-59.
[3] Unno K, Fujitani K, Takamori N, et al. Theanine intake improves the shortened lifespan, cognitive dysfunction and behavioural depression that are induced by chronic psychosocial stress in mice[J]. Free Radic Res, 2011, 45(8): 966-974.
[4] Yin C, Gou L, Liu Y, et al. Antidepressant-like effects of L-theanine in the forced swim and tail suspension tests in mice[J]. Phytother Res, 2011, 25(11): 1636-1639.
[5] Egashira N, Ishigami N, Pu F, et al. Theanine prevents memory impairment induced by repeated cerebral ischemia in rats[J]. Phytother Res, 2008, 22(1): 65-68.
[6] Kakuda T.Neuroprotective effects of theanine and its preventive effects on cognitive dysfunction[J]. Pharmacol Res, 2011, 64(2): 162-168.
[7] Takeda A, Sakamoto K, Tamano H, et al. Facilitated Neurogenesis in the Developing Hippocampus After Intake of Theanine, an Amino Acid in Tea Leaves, and Object Recognition Memory[J]. Cell Mol Neurobiol, 2011, 31(7): 1079-1088.
[8] Ritsner MS, Miodownik C, Ratner Y, et al. L-theanine relieves positive, activation, and anxiety symptoms in patients with schizophrenia and schizoaffective disorder: an 8-week, randomized, double-blind, placebo-controlled, 2-center study[J]. J Clin Psychiatry, 2011, 72(1): 34-42.
[9] Cross DR, Kellermann G, McKenzie LB, et al. A randomized targeted amino acid therapy with behaviourally at-risk adopted children[J]. Child: care, health and development, 2011, 37(5): 671-678.
[10] Yamada T, Nishimura Y, Sakurai T, et al. Administration of theanine, a unique amino acid in tea leaves, changed feeding-relating components in serum and feeding behavior in rats[J]. Biosci Biotechnol Biochem, 2008, 72(5): 1352-1355.
[11] Zheng G, Sayama K, Okubo T, et al. Anti-obesity effects of three major components of green tea, catechins, caffeine and theanine, in mice[J]. In Vivo, 2004, 18(1): 55-62.
[12] Borzelleca JF, Peters D, Hall W.A 13-week dietary toxicity and toxicokinetic study with l-theanine in rats[J]. Food Chem Toxicol, 2006, 44(7): 1158-1166.
[13] Yamada T, Terashima T, Wada K, et al. Theanine, r-glutamylethylamide, increases neurotransmission concentrations and neurotrophin mRNA levels in the brain during lactation[J]. Life Sci, 2007, 81(16): 1247-1255.
[14] Yamada T, Terashima T, Honma H, et al. Effects of theanine, a unique amino acid in tea leaves, on memory in a rat behavioral test[J]. Biosci Biotechnol Biochem, 2008, 72(5): 1356-1359.
[15] Nishida K, Yasuda E, Nagasawa K, et al. Altered levels of oxidation and phospholipase C isozyme expression in the brains of theanine-administered rats[J]. Biol Pharm Bull, 2008, 31(5): 857-860.
[16] Horner KM, Byrne NM, Cleghorn GJ, et al. The effects of weight loss strategies on gastric emptying and appetite control[J]. Obes Rev, 2011, 12(11): 935-951.
[17] Jiang C, Fogel R, Zhang X.Lateral hypothalamus modulates gut-sensitive neurons in the dorsal vagal complex[J]. Brain Res, 2003, 980(1): 31-47.
[18] Grabauskas G, Moises HC.Gastrointestinal-projecting neurones in the dorsal motor nucleus of the vagus exhibit direct and viscerotopically organized sensitivity to orexin[J]. J Physiol, 2003, 549(Pt 1): 37-56.
[19] Kakuda T, Nozawa A, Sugimoto A, et al. Inhibition by theanine of binding of [3H]AMPA, [3H]kainate, and [3H]MDL 105,519 to glutamate receptors[J]. Biosci Biotechnol Biochem, 2002, 66(12): 2683-2686.
[20] Hettes SR, Gonzaga WJ, Heyming TW, et al. Stimulation of lateral hypothalamic AMPA receptors may induce feeding in rats[J]. Brain Res, 2010, 1346: 112-120.
[21] Fetissov SO, Meguid MM, Sato T, et al. Expression of dopaminergic receptors in the hypothalamus of lean and obese Zucker rats and food intake[J]. Am J Physiol Regul Integr Comp Physiol, 2002, 283(4): R905-R910.
[22] Imre G, Fokkema DS, Den Boer JA, et al. Dose-response characteristics of ketamine effect on locomotion, cognitive function and central neuronal activity[J]. Brain Res Bull, 2006, 69(3): 338-345.
[23] Jentsch JD, Roth RH.The neuropsychopharmacology of phencyclidine: from NMDA receptor hypofunction to the dopamine hypothesis of schizophrenia[J]. Neuropsychopharmacology, 1999, 20(3): 201-225.